Quantification of Human Chorionic Gonadotropin by Bovine Serum Albumin Nanoparticles

Author(s):

Ali Jebali * , Bahram Kazemi , Seyedhossein Hekmatimoghaddam

Abstract:
Background And Aims
Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs).
Materials And Methods
At first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. Then the concentration of β-subunit of human chorionic gonadotropin (βHCG) in the clinical samples was quantified by traditional enzyme-linked immunosorbent assay (ELISA), and then checked by the nano-immunoassay.
Results
The Pearson’s correlation coefficient between ELISA and nano-immunoassay was high, i.e., 0.80. Relative sensitivity and specificity of this nano-immunoassay were reported 97.4% and 96.6%, respectively.
Conclusions
BSA NPs can be applied in nano-immunoassays as a new structure, and as an example, βHCG can be detected by this novel assay.
Keywords:

Bovine serum albumin , Human chorionic gonadotropin , Nano , immunoassay , Nanoparticles

Language:
English
Published:
International Journal of Medical Laboratory, Volume:3 Issue: 1, Feb 2016
Pages:
13 to 19
https://magiran.com/p1513399  
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