amin jalili

MicroRNAs, which are micro-coordinators of gene expression, have been recently investigated as a potential treatment for cancer. The study used computational techniques to identify microRNAs that could target a set of genes simultaneously. Due to their multi-target-directed nature, microRNAs have the potential to impact multiple key pathways and their pathogenic cross-talk. 

We identified microRNAs that target a prostate cancer-associated gene set using integrated bioinformatics analyses and experimental validation. The candidate gene set included genes targeted by clinically approved prostate cancer medications. We used STRING, GO, and KEGG web tools to confirm gene-gene interactions and their clinical significance. Then, we employed integrated predicted and validated bioinformatics approaches to retrieve hsa-miR-124-3p, 16-5p, and 27a-3p as the top three relevant microRNAs. KEGG and DIANA-miRPath showed the related pathways for the candidate genes and microRNAs

The Real-time PCR results showed that miR-16-5p simultaneously down-regulated all genes significantly except for PIK3CA/CB in LNCaP; miR-27a-3p simultaneously down-regulated all genes significantly, excluding MET in LNCaP and PIK3CA in PC-3; and miR-124-3p could not down-regulate significantly PIK3CB, MET, and FGFR4 in LNCaP and FGFR4 in PC-3. Finally, we used a cell cycle assay to show significant G0/G1 arrest by transfecting miR-124-3p in LNCaP and miR-16-5p in both cell lines. 

Our findings suggest that this novel approach may have therapeutic benefits and these predicted microRNAs could effectively target the candidate genes.

The most common bacterium linked to the hospital and pneumonia-related infections is Pseudomonas aeroginosa, a known opportunistic pathogen. High mortality rates are brought on by this bacterium in hospitalized patients, immunocompromised individuals, and cystic fibrosis patients. The formation of biofilm structures has also made it more challenging to treat patients. Due to the severe side effects of the antibiotics, the lack of efficacy due to resistance, and the well-documented antibacterial effects of traditional plants, this study investigated the antibacterial properties of the combination of thyme, rosemary, and green tea. Therefore, the main purpose of the current study was to examine the antibacterial and anti-biofilm effects of the extracts of these three plants.

Pseudomonas arginosa strains were obtained from patients at Ghaem Hospital, Mashhad, Iran. The efficacy of various antibiotics on different strains was studied, and bacterial strains were classified into sensitive, semi-sensitive, and resistant. The minimum inhibitory concentration (MIC) of the combination of the three extracts, as well as the antibiotic of choice, was determined. The effect of the extract on the biofilm formation was also investigated.

The results indicated that the ternary composition has significant antibacterial and anti-biofilm effects; also, the combination of these three extracts has shown significant synergistic properties with each other. Further, the extracts inhibited the formation and growth of the biofilm.

Results of this study indicated that the optimum combination of the three extracts possessed a remarkable antibacterial and anti-biofilm activity. Further, we have shown the synergistic antibacterial activity of the three extracts improved in the presence of the antibiotic of choice, resulting in a decrease in the MIC value of the antibiotic.

Bacterial infection remains the most frequent complication of burn injury, which can lead to sepsis, evenif antibiotics are used topically and systemically. Pseudomonas aeruginosa (P. aeruginosa) is the main causative agent inmany cases. The emergence of antibiotic-resistant strains in recent years has increased the need to find novel alternativetherapies, such as probiotics. Therefore, this study aimed to examine the antimicrobial properties of probiotic cell-freesupernatant (CFS), along with the potential use of a chitosan scaffold both as an antimicrobial agent and as a carrier forthe delivery of these complexes

Evaluation of the antimicrobial properties of cell-free soluble factors of probiotic bacteria both alone and incombination with chitosan scaffolds

Nine isolates of P. aeruginosa previously identified by standard diagnostic tests were investigated.The antimicrobial effects of probiotics in the form of Pedilact® oral drop which contained three probiotic strains,Kidilact® sachet, which contained seven probiotic strains, and strains of Lactobacillus casei (L. casei) and Lactobacillusacidophilus (L. acidophilus) isolated from yogurt were studied by an agar well diffusion assay and by using CFS harvestedat various growth stages, without pH neutralization. Chitosan with different concentrations of glutaraldehyde (GA) as acrosslinking agent was fabricated to produce a suitable scaffold for loading cell-free supernatants of probiotic strains. The scaffolds were then characterized using scanning electron microscopy. The antimicrobial properties of the CFS, chitosan, and chitosan scaffolds loaded with CFS were analyzed against MDR P. aeruginosa.

In the agar well diffusion assay, CFS obtained from probiotic strains effectively inhibited the growth of a clinicalstrain of P. aeruginosa. This effect was observed when CFS was assessed without pH neutralization. Kidilact® was the most promising synbiotic formulation based on its inhibitory activity. The chitosan scaffold was successfully fabricated, as shown by SEM, and its structure was not affected by acidic CFS. The fabricated scaffolds were able to deliver CFS and, interestingly, antibacterial activity against P. aeruginosa when CFS was loaded on the chitosan scaffold was enhanced significantly.

The results of this study showed chitosan scaffold loaded with cell-free probiotics metabolites can beconsidered to be a promising antimicrobial dressing in wound healing applications

True repentance is rebirth. The great change in the soul of the repentant person causes him to wisely and unhesitatingly accept responsibility for his mistake and seek to correct the past and prepare for the future. The action of the criminal legislature to limit the influence of the institution of repentance in the partial fall of the government's punitive punishments and the inability of the judiciary to issue a moratorium on prosecution is contrary to the rationality and spirituality hidden in Islamic criminal policy and the judiciary being a judge. The organization of the judicial cycle of the Islamic Republic of Iran on the criminal policy of Islamic punishment requires the reliance of the penitentiary on the criminal response to the act of punishment. Therefore, it is appropriate for the divine institution of repentance in criminal law to be the muscat of punishment for all punitive crimes of the government by all judicial authorities. Avoiding criminalism and paying attention to moral values ​​and social interests in the legislative stage and preventing procrastination and increasing the volume of cases in the executive dimension, are among the goals of the author to take this position.

Human dignity, as the supreme divine gift, demands more and more respect and observance of the rights of individuals in the living world today. Reflection on the latest law in the field of criminal procedure in comparison with the previous drafting laws shows the tendency of the criminal legislator to observe the defense rights of the suspects and the accused as much as possible and in the stages under preliminary investigation.The Code of Criminal Procedure adopted in 1392, enumerating the rights of the accused, the right to inform the accused, the right to declare the rights of the accused, the right to have a lawyer, the right to study and access the case, the right to inform the family, the right to medical examination and the right to remain silent. It has taken a big step towards justice. In the meantime, the lack of guarantees for proper performances, such as the invalidity of the preliminary investigation on the assumption of non-compliance with the defense rights of the accused by law enforcement and judicial authorities, has made it impossible to consider Iranian protectionist laws as defendant of the accused. The author, by first enumerating the types of defense rights contained in the Code of Criminal Procedure adopted in 1392 and comparing these rights with the protection laws of countries such as the United States and Britain, seeks to reveal the protection laws intended for the accused in the preliminary investigation stage and Finally, the policy on the extremely important issue of guaranteeing the implementation of the defense rights of the master in the law is examined.

In order to sustain the world’s growing population, the production of food must be more than 70% of what it is produced today. A remarkable and stable solution for the eradication of hunger is using genetically modified organisms (GMOs). Genetic modifications have contributed to larger yields in agriculture and livestock as well as the development of new biopharmaceuticals, vaccines and proteins. Despite the large benefits thatgenetic engineering and GMOs can bring for us, they may produce some hazards and concerns in certain areas. Moreover, considering the products to be new and also vast changes that they can make in economical, social, health, and safety matters, ethical issues plays an important role in the application of these technologies.

This is a review study. Research data were collected by searching databases of Web of science, PubMed, Scopus, google scholar, Magiran (Bank of Iran's Magazines Information), SID (Jahad-e Daneshgahi Scientific Information Center) with keywords of Ethic, Genetic engineering, genetically modified organisms and transgenic.EthicalConsiderations:The findings of these articles are stated in all stages of the research without bias and with respect to ethical aspects.

In this study, first some of the beneficial effects of such products on humanity and their contribution to the conservation of available resources are pointed outand thenpossible concerns aboutmass production and consumption of transgenic products as well as safety issues are discussed. Finally, the need to observe ethical considerations, the importance of respecting the rights of customers and product labeling are mentioned.

Adopting rational and prospective policies by observing ethical aspects, enacting integrated laws to avoid consumer's confusion, observing ethical aspects and providing correct and unbiased informing can prevent the possible dangers of this technology and peoplewill profit its benefits.

Genital infection caused by Herpes simplex virus (HSV) is one of the most common health problems, worldwide. Several methods such as cell culture, serological and molecular methods have been used to detect this virus. Currently, Real-Time Polymerase Chain Reaction (Real time-PCR) technique is widely used due to its high sensitivity and specificity. Besides, Real time-PCR can be employed in the follow-up of therapeutic effects in HSV-infected person who is being treated with antiretroviral drugs. We conducted a review on traditional and current diagnostic methods with a focus on their limitations in the diagnosis of HSV infection.

Polyethylenimine (PEI) is one of the most widely used polymers in gene delivery. The aim of this study was to modify PEI by replacing some of its primary amines with Brevinin 2R (BR-2R) peptide in order to increase the efficiency of gene delivery. Polyethylenimine was modified by BR-2R peptide by two different approaches; A) conjugation methods including (І) using succinimidyl 3-(2-pyridyldithio) propionate (SPDP), (П) EDC/NHS protocol and (ПІ) EDC/NHS+6-bromohexanoic acid protocol, and B) physical interaction method. The modified polymers were characterized for their ability of plasmid condensation, number of primary amines, size and zeta potential. The transfection efficiency and cytotoxicity were evaluated on HEK293, L929, WEHI164 and Neuro2A cell lines by green fluorescent protein (GFP)-based plasmid (pGFP) reporter gene and viability assays, respectively. Apoptosis induction ability was also evaluated via PI/Annexin V assay. Polyplex had size and zeta potential between 200-270 nm and +21.5- +28.4 mV, respectively. All vectors were able to condense plasmid DNA in C/P=4 (carrier-plasmid ratio). Transfection results on the Neuro2A cell line showed that the vector containing the BR-2R peptide, which was synthesized using EDC-NHS protocol had the best transfection efficiency. Our results showed that conjugation of Brevinin 2R as cell penetrating peptide to polyethyleneimine could enhance the transfection ability of the polymer.

Aims of study: Worldwide, hepatitis C virus (HCV) infection is a serious public health disease unlike hepatitis A and B, there is currently no vaccine against HCV available. Thus, extensive studies are under way to design new and effective treatments against HCV. Core protein is a component of HCV particle which is the first antigen recognized by the immune system.beside protective properties of core protein, anti –core antibodies can be used to monitor the disease progress. The purpose of the present study was to isolate and clone the core (C) gene from HCV genotype 1a in an attempt to construct a recombinant vector and subsequently evaluate its expression in a cell culture system.
MethodsRNA genome of HCV genotype 1a was extracted from the blood of an infected patient. Complementary DNA (cDNA) was synthesized. HCV 1a core gene was amplified by PCR using specific primers and it was cloned into a eukaryotic expression vector. Huh7.5 cells were transfected by the designed recombinant vector and the cellular expression of the core gene was confirmed by RT-PCR.
Recombinant pcDNA3.1 () vector containing the HCV core gene with approximate size of 576bp was successfully designed. RT-PCR was used to confirm the expression of core antigen in an Huh7.5 cell line.
The results showed that the core gene was successfully isolated from HCV genotype 1a and was cloned into the eukaryotic expression vector. This recombinant vector effectively replicated in Huh7.5 cell line. and its protective and therapeutic effects can be examined in further investigations.