فهرست مطالب daryoush shahbazi gahrouei

The use of radiopharmaceuticals in nuclear medicine departments and their harmful biological effects on patients and their relatives are necessary to assess the dose in the Nuclear Medicine Department, Chamran Hospital, Isfahan, and compare with permitted values.

In this experimental cross-sectional study, the cumulative dose of 15 personnel and 30 patients referred to Chamran Hospital in Isfahan with thyroid cancer who were candidates for receiving 200 mCi of iodine 131 and overnight quarantine was investigated. For this purpose, thermoluminescence dosimeters (TLDs) were used. TLDs were placed at distances of 0.5, 1, and 1.5 meters from the patients' heads, and then they were collected and read, and the values were compared with permitted doses.

The cumulative dose of gonads, thyroid, two thumbs, and index finger of personnel was 0.113 ± 0.11, 0.134 ± 0.09, 0.501 ± 0.12 mSv, and found that the annual dose is 1.356, 1.608, and 6.012 mSv, respectively. The average cumulative dose of the patients at distances of 0.5, 1, and 1.5 meters from the patients was 49.12 ± 5.74, 12.73 ± 4.43 and 3.53 ± 4.37 mSv, respectively.

The dose of the fingers of personnel was within the boundary of permitted doses, so it is necessary to recommend using lead gloves in possible cases. The minimum distance required to minimize the risks caused by iodine 131 is 150 cm, and it seems crucial to observe this distance in the first five days of discharge of patients.

Magnetic Resonance imaging (MRI) is a valuable diagnostic tool by its non-invasive/non-ionizing nature.

This study aims to determine justification of MRI in hospitalized patients at a tertiary provincial referent medical center in a one-year period.

In the present retrospective and descriptive cross-sectional study, 438 admitted patients referred for MRI during 2017 were selected using systematic random sampling. The age, gender, investigated organ, the specialty of requesting physician, MRI with and without contrast, MRI diagnostic outcome were collected using checklists. Descriptive statistics and chi-square test were used for data analysis.

The mean age of the patients was 42±26 years-old and female represented 53% of enrolled patients. The most and less prevalent investigated organs were the cerebrum and the orbit. After excluding cancer diagnosis, cancer staging, and therapeutic follow-up exams, MRI request was oriented in 64.3% and 77.2% of positive results was concordant with aforementioned diagnostic orientation (P<0.001). Oriented diagnostic MRI requesting is influenced by age, medical specialists and, investigated organ (P<0.001). The positive MRI is influenced significantly by oriented MRI request, gender, medical specialists and investigated organ (P<0.001). The diagnosis concordance of MRI is influenced significantly by oriented MRI request, medical specialists and investigated organ (P<0.001). 

Appropriate implementation of medical imaging requires boosting employed rationality by the concerned physicians. The current suboptimal results to requesting MRI rationality should mandate supplementary educational programs as to incite the medical corpus more closely implementing the published medical practice guidelines.

Laryngeal damages after chemoradiation therapy (RT) in nonlaryngeal head‑and‑neck cancers (HNCs) can cause voice disorders and finally reduce the patient’s quality of life (QOL). The aim of this study was to evaluate voice and predict laryngeal damages using statistical binary logistic regression (BLR) models in patients with nonlaryngeal HNCs.

This cross‑section experimental study was performed on seventy patients (46 males, 24 females) with an average age of 50.43 ± 16.54 years, with nonlaryngeal HNCs and eighty individuals with assumed normal voices. Subjective and objective voice assessment was carried out in three stages including before, at the end, and 6 months after treatment. Eventually, the Enter method of the BLR was used to measure the odds ratio of independent variables.

In objective evaluation, the acoustic parameters except for F0 increased significantly (P < 0.001) at the end treatment stage and decreased 6 months after treatment. The same trend can be seen in the subjective evaluations, whereas none of the values returned to pretreatment levels. Statistical models of BLR showed that chemotherapy (P < 0.05), mean laryngeal dose (P < 0.05), V50 Gy (P = 0.002), and gender (P = 0.008) had the greatest effect on incidence laryngeal damages. The model based on acoustic analysis had the highest percentage accuracy of 84.3%, sensitivity of 87.2%, and the area under the curve of 0.927.

Voice evaluation and the use of BLR models to determine important factors were the optimum methods to reduce laryngeal damages and maintain the patient’s QOL.

Due to the limitations of CT imaging, it is necessary to use magnetic resonance imaging (MRI) as a complementary method in the treatment planning process. This study aimed to compare contouring and treatment planning based on MRI/CT images with contouring and treatment planning based on CT images in the treatment of rectal cancer using helical tomotherapy.

In this prospective study, 12 patients with rectal cancer underwent MRI diagnostic imaging and CT imaging was also performed at an interval of one day. The contouring process was performed for each patient based on both methods. After that, a dose of 45 Gy was delivered to the planning target volume (PTV). Finally, the size of the treatment volumes and the parameters Dmean, V45, HI, CI, and D98% were extracted and compared from the treatment planning system.

CT-based contouring method compared to MRI/CT-based method showed higher averages for treatment volumes. In addition, in CT-based plans compared to MRI/CT-based plans, the average CI, V45, and D98% were significantly lower and the average HI was significantly higher.

The results of this study show that contouring based on MRI/CT images can estimate the size of treatment volumes smaller than contouring based on CT. Also, treatment plans based on MRI/CT images can provide more appropriate dose coverage for PTV.

Nasopharyngeal cancer is one of the common head and neck cancers and the main treatment for this type of cancer is radiotherapy. The purpose of this study is to investigate and compare the dose of organs at risk in two adaptive three-dimensional methods (3D-CRT) and helical tomotherapy (HT) in patients with head and neck cancers.

This study is a cross-sectional experimental retrospective study and to conduct this study, CT scan images (Computed Tomography) of 16 patients with early stages of NPC with an average age range of 16-81 years (42.7 ± 16.3) those were randomly selected. The organs at risk and their target volume were contoured and the treatment plan of the patients was done for both HT and 3D-CRT methods. In the end, using a Volume-Dose histogram (DVH), the dosimetry variables of organs at risk and target volume were extracted and compared with each other.

Compared to 3D-CRT, the HT method improved the homogeneity index and concordance index. It also significantly reduced the dose of the studied OARs compared to 3D-CRT, although the dose of some of these organs exceeded the prescribed dose limit.

HT method is a better technique for treating nasopharyngeal cancer patients who are candidates for radiotherapy due to better coverage of the target volume and also, better performance in preserving OARs compared to the 3D-CRT method.

Head and Neck cancers remain a significant problem due to their high morbidity and mortality and in most cases, require regional lymph nodes radiotherapy treatment. This work aims to evaluate and compare two methods of Three-Dimensional Conformal Radiation Therapy (3D-CRT) and Helical Tomotherapy (HT) based on treatment planning and selection of the most appropriate method to reduce side effects.

In this study, 20 patients with head and neck cancers were considered for treatment in the radiotherapy department of Seyed Alshohada Hospital, Isfahan, Iran. Two methods of HT and 3D-CRT were done using a 6 MeV photon energy apparatus and specific treatment planning algorithms. The dose-volume distribution curve (DVH), the dosimetric variables of the target organ, the radiation delivery efficiency, and the organs at risk were determined and results were compared with each other.

In HT the dose received by %95 planning target volume (PTV) and value doses of %2 and %98 showed significant improvement over 3D-CRT. For all studied organs at risk (OARs), the decrease in Dmax of HT was significant compared to 3-DCRT.

Helical tomotherapy enhances the target volume coverage and offers a good dose distribution. HT compared to 3D-CRT is a desirable method in treating head and neck cancer patients with the involvement of regional lymph nodes.

Glioblastoma multiforme (GBM) is the most common and malignant brain tumor. The current standard of care is surgery followed by radiation therapy (RT). Radiotherapy treatment plan evaluation relies on radiobiological models for accurate estimation of tumor control probability (TCP). This study aimed to assess the impact of obtained magnetic resonance imaging (MRI) data before and 12 weeks after RT to achieve the optimum TCP model to improve dose prescriptions in radiation therapy of GBM.

In this quasi‑experimental study, MR images and its relevant data from 30 patients consisting of 9 females and 21 males (mean age of 46.3 ± 15.8 years) diagnosed with GBM, whose referred for radiotherapy were selected. The data of age, gender, tumor size, volume, and signal intensity using analysis of MRI data pre‑ and postradiotherapy were used for calculating TCP. TCP was calculated from three common radiobiological models including Poisson, linear quadratic, and equivalent uniform dose. The impact of some radiobiological parameters on final TCP in all patients planned with three‑dimensional conformal radiation therapy was obtained.

A statistically significant difference was found among TCP in Poisson model compared to the other two models (P < 0.001). Changes in tumor volume and size after treatment were statistically significant (P < 0.05). Different combinations of radiobiological parameters (α/β and SF2 in all models) observed were meaningful (P < 0.05).

The results showed that among TCP radiobiological models, the optimum is the Poisson. The results also identified the importance of TCP radiobiological models in order to improve radiotherapy dose prescriptions.

Application of nanoparticles have in the core of researchers attention for both imaging and therapy of cancers. This review article aimed to prepare an outline on recent applications of iodine nanoparticles (INPs) as theranostic agents in both diagnosis and therapies. Among various strategies are used in treatment of cancers, radiotherapy with radiopharmaceutical agents especially radioisotope of iodine displays satisfactory results for numerous types of cancers. In recent years, new investigations were done in order to develop the novel structure of INPs. These nanoprobes could act as efficient theranostic purposes. Iodine nanoparticles may be applied in nuclear medicine imaging and may be effective with mega voltage (MV) photons in cancer therapy, but this remains to be tested with different cancer cells. By using INPs, effective steps can be taken in the future in both diagnosis and treatment of cancers. This review emphasized the recent research findings on the application of INPs in medical imaging and therapeutic of cancers. The current challenges and the perspectives for their future applications were also represented and discussed.

In this study, superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with anti-epidermal growth factor receptor antibody (anti-EGFR-SPIONs) were used as a targeted contrast agent in magnetic resonance imaging (MRI) in mice.

This was an experimental intervention, performed on 25 C57BL/6 healthy and cancerous mice on LL/2 (LLC1) cell line. Atomis absorption spectrophotometry (AAS) was used to determine of iron content in tumor and other studied tissues and MRI scanner was applied for MR imaging signal intensity under in-vivo conditions.

The results of AAS showed a good iron uptake using specific antibody in the tumor compared to other organs. Moreover, image signal intensity findings revealed its targeted diagnosis for lung cancer LLC1 cells.

Designed nanoprobes may be used as a diagnostic agent in detection of target cells and as a contrast agent for targeted magnetic resonance imaging of these cells.

Radioactive release materials in nuclear facilities causes environmental pollution, and absorption of radiations by respiration by people and staff in the area of the accident is a vital problem. The aim of this study was to stimulate the event using Radiological assessment system for consequence analysis (RASCAL) software and compare the results with those of Gaussian experimental model.

In this study, the accident included Uanium hexafluoride (UF6) gas leakage in the environment from the storage areas of the Sooreh Company, Isfahan, Iran. The geographical distribution of radioactive material and the received amount of staff and people around the site were estimated at a radius of ten kilometers.

Uranium hexafluoride gas leakage event had the highest amount of radioactivity release that caused absorbed dose to people and staff in the company fence line.

Findings showed that Uranium hexafluoride would not threaten any deterministic effects on staff and people. Also, it is recommended to use software codes to calculate event and radiation risk.

None of the molecular imaging modalities can produce imaging with both anatomical and functional information. In recent years, to overcome these limitations multimodality molecular imaging or combination of two imaging modalities can provide anatomical and pathological information.

Magnetic iron oxide nanoparticles were prepared by co‑precipitation method and then were coated with silica according to Stober method. Consequently, silica‑coated nanoparticles were amino‑functionalized. Finally, gold nanoparticles assembled onto the surfaces of the previous product. Cytotoxicity effects of prepared Fe3O4@Au nanoparticles were evaluated by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay on human hepatocellular carcinoma cells. Their ability as a dual‑mode contrast agent was investigated by magnetic resonance (MR) and computed tomography (CT) imaging.

Fe3O4@Au nanoparticles were spherical undersize of 75 nm. X‑ray diffraction analysis confirmed the formation of Fe3O4@Au nanoparticles. The magnetometry result confirmed the superparamagnetism property of prepared nanoparticles, and the saturation magnetization (Ms) was found to be 33 emu/g. Fe3O4@Au nanoparticles showed good cytocompatibility up to 60 μg/mL. The results showed that the Fe3O4@Au nanoparticles have good r2relaxivity (135.26 mM−1s−1) and good X‑ray attenuation property.

These findings represent that prepared Fe3O4@Au nanoparticles in an easy and relatively low‑cost manner have promising potential as a novel contrast agent for dual‑modality of MR/CT imaging

The aim of the study was to evaluate the potential of manganese‑zinc ferrite nanoparticles (MZF NPs) as a novel negative magnetic resonance imaging (MRI) contrast agents for 4T1 (mouse mammary carcinoma) and L929 (murine fibroblast) cell lines.

MZF NPs and its suitable coating, polyethylene glycol (PEG) via covalent bonding, were investigated under in vitro condition. The cytotoxicity of MZF NPs was tested by 3‑(4,5‑dimethyl thiazolyl‑2)‑2,5‑diphenyltetrazolium bromide assay after 12 and 24 h of incubation. To evaluate the potential of MZF NPs as T2 MRI nanocontrast agent, images were obtained from phantom containing different Fe concentrations and T2 relaxivity (r2) was measured. The viability of both 4T1 breast cancer and L929 murine fibroblast cell lines incubated with different Fe concentrations.

In vitro T2‑weighted MRI showed that signal intensity of 4T1 cells was lower than that of L929 as control cells. T2‑weighted MRI showed that signal intensity of MZF NPs enhanced with increasing concentration of NPs. The values of 1/T2 relaxivity (r2) for coated MZF NPs with PEG found to be 85.5 mM−1 s−1 which is higher than that of commercially clinical used (Sinerem) MRI contrast agent.

The results showed that MZF NPs have potential to detect breast cancer cells (4T1) and also have high contrast resolution between normal (L929) and cancerous cells (4T1) which is a suitable nanoprobe for T2‑weighted MR imaging contrast agents.

Thyroid exposure to radiation in brain computed tomography (CT) scan is of great value since it is considered as a vital organ. This study aimed to investigate the absorbed dose of thyroid by various protocols of head CT in patients referring to 64-slice CT scan center and to compare the values with the calculated dose by imaging performance and assessment of CT (ImPACT) method. Also, the values of CT scan dose index (CTDI) were calculated with semiconductor detector. In this cross-sectional study, 120 outpatients including three groups of forty individuals over 40 years old referring to the hospital radiology centers in Tehran for head CT were chosen and 3 thermo-luminescence dosimeter (TLD-GR200) were applied on thyroid gland of each patient. For brain CT, Absorbed and effective doses of thyroid gland were calculated by ImPACT software. In addition, semiconductor detector in head CTDI phantom calculated CTDI for the applied protocols. Mean effective dose of thyroid gland in brain scan group was calculated by TLD and ImPACT software which showed no significant difference (P < 0.001). Mean effective dose of thyroid gland in unidirectional and bi-directional sinus scan by TLD and ImPACT software were different significantly (P < 0.001). Also, the differences between CTDI values shown by brain and sinus scan protocol with semiconductor detector and those CTDI were significant (P < 0.001). The calculated values of absorbed dose and effective doses of thyroid by TLD and ImPACT software were not significantly different. Mean effective dose calculated for thyroid gland in head scans by TLD and ImPACT was less than the annual permissive level for thyroid gland suggested by International Committee on Radiological Protection. In this study, calculated values of thyroid effective dose in brain scan with 64-slice scanner were less than the calculated values in a similar study.

Medical imaging modalities are used for different types of cancer detection and diagnosis. Recently, there have been a lot of studies on developing novel nanoparticles as new medical imaging contrast agents for the early detection of cancer. The aim of this review article is to categorize the medical imaging modalities accompanying with using nanoparticles to improve potential imaging for cancer detection and hence valuable therapy in the future. Nowadays, nanoparticles are becoming potentially transformative tools for cancer detection for a wide range of imaging modalities, including computed tomography (CT), magnetic resonance imaging, single photon emission CT, positron emission tomography, ultrasound, and optical imaging. The study results seen in the recent literature provided and discussed the diagnostic performance of imaging modalities for cancer detections and their future directions. With knowledge of the correlation between the application of nanoparticles and medical imaging modalities and with the development of targeted contrast agents or nanoprobes, they may provide better cancer diagnosis in the future.

The central role of molecular imaging modalities in cancer management is an undeniable fact that could help to diagnose cancer tumors in early stages. The main aim of this study is to prepare a novel targeted molecular imaging nanoprobe of CD24-PEGylated Au NPs to improve the ability of Computed tomography scanning (CT scan) outputs for both in vitro and in vivo detection of breast cancer (4T1) cells.
Gold nanoparticles (Au NPs) were synthesized and coated with polyethylene glycol (PEG) chains in order to improve the stability of the Au NPs and to provide bio modification points for antibody immobilization. The synthesized nanoprobe was used for both in vitro and in vivo targeted CT imaging breast cancer cells (4T1) and the xenograft tumor model.
Findings showed that the attenuation coefficient of 4T1 cells that were targeted by CD24-PEGylated Au NPs is calculated to be over two times higher than the untargeted 4T1 cells (15 HU vs 39 HU, respectively). Indeed, the results clearly reveal that the developed CD24-PEGylated Au NPs showed the tumor CT enhancement was higher than that of Omnipaqe which used as control.
Also, the CT number values of the tumor area at different time points gradually increased after 60 min post injection and was significantly higher than before injection.
Results showed the introduced CT imaging nanoprobe (Au NPs-PEGylated) could be useful for CT imaging of breast tumors under in vivo condition. Overall, it is concluded that Au NPs-PEGylated contrast media is able to detect breast cancer (4T1) cells and is more effective compared with other casual methods.

MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] is a sensitive and accurate method to determine survival fraction of irradiated cancer cells. The aim of present study was to evaluate the radiosensitivity of cancer cells X-ray Irradiation in comparison to normal cells using the MTT assay.
Four cancer cell lines were used, the mouse breast 4T1 cells, the mouse fibroblast L929 cells, the human gastric AGS cells, and the human dermal fibroblasts (HDF) cells. For each cell line, MTT assay was carried out after irradiation to 0, 2, 4, and 6 Gy. For MTT assay, the relationship between absorbed dose and cell number, optimal seeding of cell number, and optimal timing of assay were determined. Then, MTT assay was performed when the irradiated cells had regained exponential growth, or when the non-irradiated cells had undergone doubling times.
Findings: With increasing radiation dose, the mortality of the cells increased, and radiation blocked cell growth.
The response of different cells to irradiation was different. MTT assay may successfully be used, and also may distinguish cell responses to different photon energies. MTT assay was undertaken with optimal assay conditions, and showed the sensitivity of cells to irradiation with regard to the plating efficiency of each cell line, and doubling time at least.

Dosimetric accuracy in intensity‑modulated radiation therapy (IMRT) is the main part of quality assurance program. Improper beam modeling of small felds by treatment planning system (TPS) can lead to inaccuracy in treatment delivery. This study aimed to evaluate of the dose delivery accuracy at small segments of IMRT technique using two‑dimensional (2D) array as well as evaluate the capability of two TPSs algorithm in modeling of small felds.
Irradiation were performed using 6 MV photon beam of Siemens Artiste linear accelerator. Dosimetric behaviors of two dose calculation algorithms, namely, collapsed cone convolution/superposition (CCCS) and full scatter convolution (FSC) in small segments of IMRT plans were analyzed using a 2D diode array and gamma evaluation.
Comparisons of measurements against TPSs calculations showed that percentage difference of output factors of small felds were 2% and 15% for CCCS and FSC algorithm, respectively. Gamma analysis of calculated dose distributions by TPSs against those measured by 2D array showed that in passing criteria of 3 mm/3%, the mean pass rate for all segment sizes is higher than 95% except for segment sizes below 3 cm × 3 cm optimized by TiGRT TPS.
High pass rate of gamma index (95%) achieved in planned small segments by Prowess relative to results obtained with TiGRT. This study showed that the accuracy of small feld modeling differs between two dose calculation algorithms.

Context: Prior research over the past few decades has shown that the hypofractionated external beam radiation therapy could be a valuable method with an outstanding potential to treat the prostate cancer. The alpha-beta ratio (α/β) is an indication of the fractionation sensitivity of a particular cell type. Values of α/β ≥ 10 Gy are responsible for early-responding normal tissues and most tumors, while values of α/β Evidence Acquisition: This review article aimed to investigation the details of radiobiological and clinical trials which are reported to highlight the characteristics of hypofractionation for the prostate cancer treatment based on the investigation of clinical results from hypofractionated treatments.
With the sufficient radiobiological and physiological research studies, it may be claimed that if α/β value for prostate cancer is less than the adjacent surrounding organs at risk and by applying fewer and larger fractions; a better therapeutic ratio can be achieved for the patient.
Radiobiological modeling can play a major role in the design of new protocols. Also, one should be aware that in modeling the hypofractionated treatment, normal tissue iso-effects are significantly important and some assumptions needed to be made regarding α/β values for the critical normal tissue complication endpoint.

Performing audits play an important role in quality assurance program in radiation oncology. Among different algorithms, TiGRT is one of the common application software for dose calculation. This study aimed to clinical implications of TiGRT algorithm to measure dose and compared to calculated dose delivered to the patients for a variety of cases, with and without the presence of inhomogeneities and beam modifiers.

Nonhomogeneous phantom as quality dose verification phantom, Farmer ionization chambers, and PC‑electrometer (Sun Nuclear, USA) as a reference class electrometer was employed throughout the audit in linear accelerators 6 and 18 MV energies (Siemens ONCOR Impression Plus, Germany). Seven test cases were performed using semi CIRS phantom.

In homogeneous regions and simple plans for both energies, there was a good agreement between measured and treatment planning system calculated dose. Their relative error was found to be between 0.8% and 3% which is acceptable for audit, but in nonhomogeneous organs, such as lung, a few errors were observed. In complex treatment plans, when wedge or shield in the way of energy is used, the error was in the accepted criteria. In complex beam plans, the difference between measured and calculated dose was found to be 2%–3%. All differences were obtained between 0.4% and 1%.

A good consistency was observed for the same type of energy in the homogeneous and nonhomogeneous phantom for the three‑dimensional conformal field with a wedge, shield, asymmetric using the TiGRT treatment planning software in studied center. The results revealed that the national status of TPS calculations and dose delivery for 3D conformal radiotherapy was globally within acceptable standards with no major causes for concern.

The head scatter factor (Sc) is important to measurements radiation beam and beam modeling of treatment planning systems used for advanced radiation therapy techniques. This study aimed to investigate the design of a miniphantom to measurement variations in collimator Sc in the presence of shielding blocks for shaping the beam using different field sizes. Copper, Brass, and Perspex buildup caps were designed and fabricated locally as material with three different thicknesses for buildup caps (miniphantoms). Measurements were performed on an Elekta Compact medical linear accelerator (6 MV) in Shafa Kerman Hospital, Iran. The Farmer-type ion chamber FG65-P (Scanditronix, Wellhofer) was used for all measurements. To measure the Sc, miniphantom was positioned in a stand vertical to the beam central axis. The data indicate that the Sc measurements using different buildup cap materials and thicknesses in 5 × 10, 7.5 × 7.5, and two 10 × 10 cm Cerrobend shield blocks ranged 0.98 to 1.00, 1.04 to 1.05, and 1.04 to 1.06, respectively. Also, it was observed that by increasing the block shield area from 50 cm2 to both 56.25 and 100 cm2, the Sc increased in all situations. Results showed that using Brass compared to Perspex and Copper has less uncertainty due to its simple preparation and cutting which is useful to measurement of variations in collimator Sc and shaping the photon beam.

All natural anticancer agents are cytotoxic basically and act mainly by the inhibition cell proliferation; but they have different mechanisms. Two assays, thiazolyl blue [3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyl-terazoliumbromide or MTT] and sulforhodamine B (SRB), are used to assess cell growth. This study aimed to compare measurements between MTT and SRB on the cancer cell lines.
Different concentrations of the bromelain were added to cultured cells including mouse breast cancer (4T1), human gastric carcinoma (AGS), and human prostate carcinoma (PC3) cell lines and incubated at 24 and 48 hours. The growth and proliferation rates of the studied cells were investigated using both MTT and SRB assays after treatment with bromelain. The differences between cells were determined using Kruskal-Wallis and Dunns tests.
Findings: Bromelain significantly decreased growth and proliferation rate of 4T1, AGS and PC3 cancer cells, in a concentration-dependent manner at different times, in both MTT and SRB assays.
Findings showed that both MTT and SRB assays gained similar data regardless of the cell types.

Magnetic resonance imaging (MRI) plays an essential role in molecular imaging by delivering the contrast agent into targeted cancer cells. The aim of this study was to evaluate the C595 monoclonal antibody-conjugated superparamagnetic iron oxide nanoparticles (SPIONs-C595) for the detection of breast cancer cell (MCF-7).
The conjugation of monoclonal antibody and nanoparticles was confirmed using X-ray diffraction, transmission electron microscopy, and photon correlation spectroscopy. The selectivity of the nanoprobe for breast cancer cells (MCF-7) was obtained by Prussian blue, atomic emission spectroscopy, and MRI relaxometry.
The in vitro MRI showed that T2 relaxation time will be reduced 76% when using T2-weighed magnetic resonance images compared to the control group (untreated cells) at the dose of 200 μg Fe/ml, as the optimum dose. In addition, the results showed the high uptake of nanoprobe into MCF-7 cancer cells.
The SPIONs-C595 nanoprobe has potential for the detection of specific breast cancer.

Electron therapy has advantages of dose uniformity in target volume and dose reduction in deeper tissues for breast cancer therapy. The aim of this study was to determine observed dose in target volume and normal tissues such as lung in breast cancer therapy after mastectomy.
The phantom was designed based on the chest wall computed tomography (CT) images after mastectomy and treatment planning software. Two linear accelerator (Neptune 10 and Saturn 20) machines were used to deliver electron beams at a dose of 200 cGy (10 and 13 MeV). Dose measurements were done using thermoluminescence dosimeter lithium fluoride chips (TLD-100: LiF: Mg; Tl).
Findings: The percentage of measured dose in internal mammary nodes, axillary nodes, and chest wall using 13 MeV was found to be 97.5, 96, and 98, respectively. In treatment with 10 MeV electron, this values changed to 99.7, 90.5, and 77 percent, respectively. The anterior lung percentage dose was 78 with 13 MeV electrons while the posterior part of lung received 47 percent with 10 MeV electron beam. Dose values for anterior and posterior part of lung changed to 83 and 45 percent, respectively.
Using 13 MeV, internal mammary and axillary lymph nodes as well as the chest wall received adequate doses but lung received excessive dose. Using 10 MeV, internal mammary nodes and chest wall were well exposed to radiation, but axillary lymph nodes did not receive enough dose. Therefore, this dose shortage should be compensated by additional posterior fields using radiation therapy with photon energies.

Accurate and fast radiation dose calculation are essential for successful radiation radiotherapy. The aim of this study was to implement a new graphic processing unit(GPU) based radiation therapy treatment planning for accurate and fast dose calculation in radiotherapy centers. A program was written for parallel running based on GPU. The code validation was performed by EGSnrc/DOSXYZnrc. Moreover, a semi-automatic rotary asymmetric phantom was designed and produced using bone, lung and soft tissue equivalent materials. All measurements was performed using a Mapcheck dosimeter. The accuracy of code was validated using the experimental data which obtained from the anthropomorphic phantom as the gold standard. Findings showed that, compared with those ofDOSXYZnrc in the virtual phantom and for most of the voxels (>95%) less than 3% dose-difference or 3 mm distance-to-agreement was found.Moreover, considering the anthropomorphic phantom, compared to the Mapcheck dose measurements less than 5% dose-difference or 5 mm distance-to-agreement was observed. Fast calculation speed and high accuracy of GPU-based Monte Carlo method in dose calculation may be useful in routine radiation therapy centers as core and the main component of a treatment planning verification system.

Computed tomography (CT) is one of the most used imaging modalities and is one of the main sources of population exposure. Recently, patient doses are estimated through CT dose index (CTDI) phantoms as a reference to calculate CTDI volume (CTDIvol) values followed by calculating equilibrium dose (Deq). This study aimed to improve measurement methods for equilibrium dose and the effective dose using the American Association of Physicists in Medicine-Task Group Report No. 111 (AAPM-TG111).
Using standard phantom of polymethylmethacrylate (PMMA) and pencil ionization chamber, the values of CTDI100 and ε(CTDI100), and DL(0) were calculated followed by calculating equilibrium dose, hence obtaining the total effective dose.
Findings: The average equilibrium dose in according to the CTDI100 and CTDIvol was found to be 26.11 and 58.99, respectively; which was based on the value of 19.4 and 42.4 mGy recorded by CT console. Differences between CTDIvol and Deq were 34.58% and 39.12% based on the experiments setting up by the CT-weighted and volume dose index, respectively.
Differences between values of CTDIvol and Deq were consistent with the literature results. Overall, weighted dose index of CT systems is not sufficient to determine patient’s dose and using new recommended indices are required.