ebrahim hoseein najdegerami

Damage due to thermal stress in the testicles is one of the causes of infertility in men and most of the mammals. Bioactive peptides have potent antioxidant activity against free radicals and reactive oxygen species. In this study, animals were randomly categorized into 6 groups; group 1 served as a control(22 ◦ C), group 2 was a bioactive peptide(10 mg kg-1 day, oral gavage, respectively) , group 3 was a heat-stressed (43 ◦ C), groups 4,5 and 6 were heat-stressed(43 ◦ C) along with bioactive peptides (5, 10 and 20 mg kg-1 day, oral gavage, respectively). After treatment (45 days), the testes were separated for histological and biochemical studies. Histopathological analysis revealed severe testicular damage following heat stress induction. Heat stress increased nitric oxide significantly(P 0/05). In the heat-stressed rats treated with 5 and 10 mg / kg bioactive peptide, no improvement was observed. Treatment of animals with 20 mg / kg showed positive effects on spermatogenesis and seminiferous tubules structure. The results of nitric oxide analysis showed a decrease in this biochemical factor following peptide treatment at a dose of 20 mg / kg(P 0/05). The results of this study showed the antioxidant effects of sardine-derived bioactive peptide on tissue and biochemical markers of testicular tissue.

Artemia is the only Crustacean that can withstand in very high salinity. Due to the climate crisis and increasing salinity of inland waters it is necessary to understand the molecular characteristics of Artemia. It can be a good way to choose the appropriate population to grow at high salinities. They are suspected of being different gene structure of Na+/ K ATPase which defer among bisexual and parthenogenetic Artemia. As a result of this molecular adaptation it seems to be seen some degrees of phenotypic adaptation which could provide difference growth abilities. In this study, two different populations of Artemia franciscana and Parthenogenetic Artemia were reared at salinities 60, 120 and 180 mg/l. At the end of the experiment the mRNA of Na+/ K ATPase was provided and converted to cDNA. Subsequent DGGE and sequencing analyses revealed that the products of these genes in A. franciscana is homozygous while in Parthenogenetic Artemia is heterozygous which is produced by two alleles. Also it was found that in the parthenogenetic Artemia protein translation and the production of two different alleles is obtained. Interestingly, no difference was observed in the samples studied. Apparently the only difference can be related to genomic variation among these populations.