فهرست مطالب f. tafvizi

Today, the use of medicinal plants in the treatment of cancer is more important due to its fewer side effects. Vascular endothelial growth factor (VEGF) is one of the most important factors in stimulating angiogenesis and promoting cancer. The aim of this study was to investigate the inhibitory effect of Valeriana Sysimberifolia root extract on proliferation of lung cancer cells (A549) and VEGF gene expression in treated cells.

The hydro alcoholic extract of Valeriana Sysimberifolia root was prepared by percolation. After preparation of cell line A549 from the Iranian Biological Resource Center, cancer cells were treated in culture medium with different concentrations of plant root extract (50, 25, 12.5, 6.25, 3.12, 1.56, 0.78 µg/ml) at 24, 48 and 72 hours and viability was performed by MTT assay at all three time. Real Time-PCR method was used to evaluate the expression of VEGF gene in cancer cells treated with IC50 concentration of plant extract.

With increasing the concentration of the extract and the treatment time, a decrease in viability of cancer cells was observed. The highest inhibition of cell proliferation was observed at a concentration of 50µg/ml after 72 hours (94% growth inhibition) which was significant compared to the control group (p= 0.0001). Also, with increasing treatment time, decreased VEGF gene expression was seen compared to the control group. Thus, a two times decrease in VEGF gene expression was seen in cells after 72 hours (p= 0.0017).

Valeriana Sysimberifolia root extract has anti-proliferative and anti-angiogenesis activity. It seems that further studies can be used as a biological product in the treatment of cancer.

The use of nanotechnology in drug delivery Not only increase the efficacy and ease of drug penetration, but also they decrease their adverse effects. In this study, TiO2 nanoparticle was used as Hydroxyl Urea carrier to increase the contact surface of the drug and cells, and with pegylation of nanoparticle surface decrease immunogenicity, hence to increase drug solubility and penetration to cells. The goal of this study was to investigate cytotoxicity of synthesized TiO2-Poly Ethylene Glycol-Hydroxy Urea (TiO2-PEG-HU) nanocomposite on Hela cell-line and apoptosis induction of treated cells compared to the control group to determine the effective dose of nanodrug. In this laboratory study, the effect of TiO2-PEG-HU nano-drug was evaluated on cells bioactivity by MTT method at concentrations of 200, 400, 800, and 1800 µg/ml in 48 and 120 hours. Annexin-V/PI flowcytometry method was used to analyze apoptosis induction. Data were analyzed using uni-directional variance and independent T-test. Higher concentrations of TiO2-PEG-HU nanocomposite decreased cells bioactivity dependent on dosage and time. As the concentration of 1600 µg/ml of nanocomposite reduced amount of bioavailability by 1.52 times over a 120-hour period compared to the 48-hour time-effect. For both times, this reduced cell survival was `significantly different from that of the control group at the level of p <0.0001. In addition, nano-drug significantly increased apoptosis induction 2.5 times in treated Hela cells (p=0.0114). Nano-composite TiO2-PEG-HU on the Hela cell line is cytotoxic and induces apoptosis and can be a promising drug for cancer treatment.