gholamreza rafiei

Anti-tumor effects of N-myc Downstream Regulated Gene2 (NDRG2) have been demonstrated in many tumors. In the present study, NDRG2 was specifically overexpressed in lung cancer cell line using Survivin Promoter (Sur-P). Then, the effects of NDRG2 overexpression on viability, apoptosis, migration, and invasion of A549 cells were evaluated.

Recombinant pAdenoVator-Sur-P-NDRG2-IRES-GFP plasmid harboring NDRG2 gene under transcriptional control of Sur-P and mock plasmid were constructed. A549 lung tumor cells and LX-2 cells (non-tumor cell line) were transfected with pAdenoVator-Sur-P-NDRG2-IRES-GFP, pAdenoVator-CMV-NDRG2-IRES-GFP, or mock plasmids. Tumor specificity of Sur-P was evaluated using fluorescent microscopy for GFP expression. The effects of NDRG2 overexpression on cell viability, apoptosis, and migration of A549 cells were measured using MTT, annexinV/7-AAD flow cytometry, and transwell migration assay, respectively. NDRG2 and matrix metalloproteinase- 2 (MMP-2) expression were measured using real time- PCR.

pAdenoVator-Sur-P-NDRG2-IRES-GFP transfection resulted in a huge GFP expression in A549 cells, but not in LX-2 cells. The results of real time-PCR analysis also showed that pAdenoVator-Sur-P-NDRG2-IRES-GFP transfection led to an abundant NDRG2 expression in A549 cells. NDRG2 overexpression decreased A549 cell viability through increasing cell apoptosis. In addition, migration, invasion, and MMP-2 expression decreased following NDRG2 overexpression in A549 cells.

The findings indicate that the targeted overexpression of NDRG2 using Sur-P can reduce the viability and invasiveness of A549 cells, suggesting possible benefits of this approach in lung cancer therapy.

The present study was conducted to evaluate the effects of nanosilver coating on the reduction of fouling fish cages of rainbow trout in the Ayush dam of Khorramabad from winter 2016 to autumn 2016. The nets were applied for antibacterial properties according to the exhaustion method The samples were collected from net panels (0.0625 m2) that were attached to the PVC frame, located at a depth of one meter from the side of the fish culture cages, and wet weight was measured, then it was identified with valid authentication keys. In this study, three genera of green algae and diatom were identified. The analyses showed that Do, temperature, and pH were positively and significantly correlated with Tetrasporaceae, wet weight and algae cover percentage. The Seasonal nanosilver panels treatment was free of fouling. Nanosilver coating can provide at least three months of effective protection against biofouling conditions. Nanosilver occupies the interstices of the polyamide polymer chain and interacts strongly with single pair electrons of nitrogen hence a coordinated complex is formed.

The aim of the present study is to investigate the effect of different levels of inorganic supplements in hydroponic system with the re-use of water on some parameters related to Nile tilapia health status. Materials &

To address that, 180 fish weighing 30±3.8 g was divided into 9 experimental units (3 for each treatment). Three different treatments were applied, including 0, 25 and 75% of nutrient requirement for hydroponic system and these levels were added to the diet of fish. Following 70 days of dietary administration, blood samples were withdrawn for caudal vein of 5 fish. Intestine sample was also taken in order to determine the bacterial microflora and histological changes.

Obtained results showed significant changed in hematological and immunological parameters in dietary treatment as compared with control (P<0.05). Total number of bacteria in intestine was significantly (P<0.05) elevated in treatment 3 as compared with other treatments. The applied diets had significant impacts on histological indices, including villi height and diameter, total number of leucocyte and mucus-secreting cells (P<0.05).

Generally, epithelium development of intestine besides the improvement of hematological parameters and stimulation of immunity system could perhaps indicate the better health status of Nile tilapia following dietary manipulation by inorganic supplementation.

Brucellosis or Malta fever is one of the most common zoonotic diseases in the world. In addition to causing human suffering and dire economic impact on animals, due to the high prevalence of Brucellosis in the western regions of Isfahan province, this study aimed to analyze effective factors in the time of Brucellosis diagnosis using parametric and semi-parametric models and to evaluate the goodness of fit of these models.

This historical cohort study, 412 patients with Brucellosis in Fereydunshahr, Iran who had referred to hospital, rural & urban health centers and physicians' private clinics in Fereydunshahr between 2006 and 2016 were recruited through census sampling. The failure (or event) in this study, was diagnosis of Brucellosis based on positive immunologic tests (2-ME test ≥1:40 and Wright serology ≥1:80). In order to eliminate  confounding variables, effective factors of the time of Brucellosis diagnosis were determined using univariate (P≤0.20) and multivariable (P<0.05) analysis according to Cox semi-parametric model and five parametric models (weibull, exponential, log-logic, log-normal and gompertz) and the best fitted model was identified. Data were analyzed using R software version 3.2.3.

According to the results of this study, occupation (farmer and livestock breeder), place of residence (urban), having a history of direct contact with livestock, simultaneous infection in other family members, and the newness of the disease (vs. recurrence) were identified as predictors of early detection of the disease. 

Despite the researchers' tendency to use Cox method in survival analysis, in this study, according to AIC, “Gopmpertz” parametric model was recognized as the best fitted regression model in the analysis of the effective factors in the definitive time of Brucellosis diagnosis.

In recent years,cases of animal bites have increased in Iran. In addition,stray animals killing had upward trend,accordingly. The incidence rate of animal bites in Fereydunshahr is higher than the national average (499 vs. 188 per 100,000),it has great importance in this regard. Therefore killing of stray animals has constantly been the agenda of the relevant organs with a high cost,but does this program reduces the rates of animal bite? This study evaluated effectiveness of stray animals' Killing on animal bites incidence in Fereydunshahr (Isfahan,IRAN) in the last 18 years (2000-17).Materials, In this routine data base study,we followed 2339 cases of animal bites (using CENSUS method) that had been diagnosed,received Anti-Rabies vaccine during the studied period,as well as 2642 cases of animal waste. The data used in this study were collected from the township health center reports. Statistical computing was performed using SPSS software (ver. 19) at an error level of 5%. Pearson's Skewness coefficient showed that both the number of animal bites,also,killed animals have normal distribution. Given the existence of parametric conditions,normal distribution,variables independence,and the interval scale of both variables,Pearson Correlation coefficient test is used to investigate the relationship. Pearson correlation analysis did not show a significant correlation between stray animals' killing with animal bites incidence (P,0.74,ɤ,-0.023). Due to 84% of animal bite cases occurred by domestic dogs in the last 18 years,killing of stray animals not only had effect on reducing the incidence of animal bite cases,but also it increases the costs,immoral methods of animal killings. The plans such as training of dog-owners (to keep in fetters of the dogs),training of the population,especially schoolchildren,can be effective in domestic dog bites reduction.

Inorganic arsenicals are worldwide environmental contaminants that affect molecular characteristics in biological systems and lead to genomic and epigenomic instability as well as epithelial mesenchymal transition (EMT). In this study, we aimed to investigate whether low levels of sodium arsenite (iAsIII) can influence EMT and genomic instability through microsatellite analysis. We have also determined epigenomic instability by investigating the methylation status of SEPT9 tumor marker in colorectal cancer (CRC) cell lines, Caco2 and HCT116, which were treated with iAsIII to assess IC50s. Short-term and long-term exposure to low concentrations (1 µM and 0.1 µM) of iAsIII in two separate experiments was implemented to analyze EMT, microsatellite status and the methylation pattern of SEPT9 promoter. As expected, after 20 days of exposure to iAsIII, the expression of CDH1 was significantly decreased while the expression of CDH2, FIB1 and VIM was increased in Caco2 and HCT116, a finding that confirmed EMT induction. However, there was no detectable alteration in the size of microsatellites. As for the methylation pattern, SEPT9 promoter was hypomethylated as a result of long-term exposure to 0.1 µM iAsIII in Caco2. Long-term exposure of HCT116 to both concentrations could induce hypomethylation of SEPT9 promoter. Our findings indicate no linkage between EMT induction and microsatellite status in iAsIII-treated CRC cell lines. For the first time, the current study has shown that the induction of EMT by iAsIII is linked with SEPT9 promoter hypomethylation in Caco2 and HCT116 in a concentration- and time-dependent pattern.

Emphasizes the Quran to know stories of the prophets, because the life of the early years of Islam, according to the subject dealt with in the books of commentary and history and stories of the prophets Written later Independent books in describing the life of the prophets Among the many stories of the prophets, manuscript Qesas Al-anbiya by"Mohammad Ibn Hassan Dayduzami" is notable. The identity of the author is unknown.All Bibliographers who have learned of his life and his work, his book titled "Nafayes al-arayes and Qesas Al-anbiya" and those considered Persian translation of Qesas Al-anbiya by Mohammd Ibn Abodolla Kisaei.From the Arabic text of Kisaei two Persian translation of a manuscript written in the seventh century AD. By comparing the Arabic text of Kisaei and Dayduzami,s book and Persian translations of his text It can be seen there is no correlation between them. This study attempts to provide reasons and evidence, stating that Qesas al-anbiy by Dayduzami is not translation of Kisaei,s book But rewriting is the book "Taj Al-Qesas" of Abunasr Ahmad Ibn Mohammad Bokhari(fourth century AD), respectively.

Qasas Ul Anbiya by Kisa''i is a works in Arabic by Muhammad b. Abdullah al-Kisa''i. The realidentityof itsauthoris not well known. This work is common in Islamiccommunities، especially among the public withnames such as“Bad’aldonya VA Qisas ul-Anbiya’’، “Qasas Ul-Anbiya va almobtada’’ and Bad al-khalq va Qasas Ul Anbiya’’. Coherence in structure of the stories has madethis book isas afictionalliterarytext. The authorhas used from theQuranic storiesandhistorical narratives in writinghisstoriesandhehas filled his book with imagination. The storiesofthisbookareconsideredintwocategoriesincluding the storiesin thecreation of the universe and (The primitive affairs، such as Divine table، pen، Throne، the empyrean، Heaven، Hell، etc.) and Storiesof the Prophets. The mostimportantpart of bookis devotedtothe story ofthe prophets. In this research، it is brieflyconsidered types of stories and itsstructuralanalysis with story elements ad mention of motifs in Qasas Ul-Anbiya by Kisa''i.

Nowadays, not only TV cables, satellites’ programs and the internet outside Iran broadcast Iranian cinematographic works without any authorization and restriction, the sale of such works is increasing in foreign countries. The breach of the creators’ rights in cinematographic works outside Iran incurs substantial damages to the cinema industry in Iran. However, these creators are not able to sue the violators and ask for compensation in the state where the rights have been breached. Currently, Iran is not a member to any copyright or related rights treaties. This Article examines and analyses the impact of the Iran’s accession to these treaties and argues that this accession plays a key role in the increase of the motivations of the creators of literary and artistic works since their rights would be protected and the scope of such protection would be expanded. This would result in the increase of the number of creative works.