javid mohseni

 The rise in bacterial resistance to antibiotics and their negative side effects has led to a growing interest in the use of medicinal plants and plant extracts. This study aimed to investigate the antibacterial effects of hydroalcoholic extract of Yellow Nasturtium on some gram-positive and gram-negative bacteria.

 In this research, after the preparation of Rosa Foetida extract, its antibacterial properties were evaluated using antibiogram, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods. Ciprofloxacin, gentamicin, and penicillin were used to determine the sensitivity of bacteria to the extract studied.

The results showed that the hydroalcoholic extract of Rosa Foetida effectively inhibited bacterial growth and viability, with a more pronounced antibacterial effect on gram-positive bacteria than on gram-negative bacteria. Using the MIC method, the extract was able to inhibit the growth of bacteria with an average of 12.5-100 mg/ml. The results of the study compared to CLSI 2018 showed that only Escherichia coli and Klebsiella pneumoniae bacteria were sensitive to ciprofloxacin and all bacteria were resistant to gentamicin and penicillin antibiotics. The results of the one-way analysis of variance revealed that there was a significant difference between the effect of extract or antibiotics against bacteria (P<0.01).

Collectively, hydroalcoholic extract of Yellow Nasturtium could be a valuable source of antibacterial activity against certain gram-positive and gram-negative bacteria. Natural antibacterial compounds are efficient for utilization in in vitro phytochemical and biological investigations.