maryam mahal dashtian

There is a fast growing tendency in the consumption of herbal remedies in the developing countries. One of the traditional medicines used for male infertility is Date palm (Phoenix dactylifera) pollen (DPP). The goal of the present study was to investigate the effect of aqueous extract of DPP on In vitro viability and proliferation rate of neonate mouse spermatogonial stem cells (SSCs). cell suspension includes sertoli cells and SSCs were isolated from neonatal 6 day-old mice testes by 2 steps enzymatic digestion. The cell suspension was cultured in DMEM and FCS 4% in the absence or presence of 0.06, 0.25 and 0.62 mg/ml of aqueous extract of DPP for 2 weeks. In order to evaluate the rate of SSCs expansion at the end of culture, the mean number of whole cells and living cells were considered as proliferation and survival rates respectively. Data analysis was done with ANOVA test. The significancy of the data was analyzed using ANOVA and Tukey post test. The results showed that there were no significant differences between the mean percent of viability and proliferation rate between control and 0.06, 0.25 and 0.62 mg/ml of DPP-treated groups (P> 0.05). Our study showed that treatment of neonatal mouse testicular cell suspension with DPP had no toxic effects on viability percent and proliferation rate of these cells. Thus, we can use DPP for evaluate the in vitro pattern of SSCs colonization in the future studies.

Spermatogenesis is a highly complex and regulated process in which germ stem cells differentiate into spermatozoa. These stem cells، called spermatogonial stem cells (SSCs)، are in the base of seminiferous tubules and have the ability of self-renewal and differentiation into functional germ cells. Due to this ability، SSCs can restore spermatogenesis after testicular damage caused by cytotoxic materials or following transplantation into an infertile recipient. Therefore، self-renewal of these cells is critical for the preservation of SSC populations and restoration of fertility. While previous studies have shown that the SSCs of mice and other species can survive and proliferate for long periods of time، little information is available about suitable culture media for the growth of human SSCs. Identification of SSC markers allows for the isolation of these populations of cells. The isolated cell can be expanded in culture and transplanted into infertile recipients. Consequently، the recognition of markers and the establishment of long-term culture systems for human SSCs will be essential for using the potential of these cells in a clinical setting. In this article، we focus on the markers that have been identified for human SSCs and in vitro culture techniques used for human SSCs proliferation.