فهرست مطالب sadegh feizollah zadeh

Celiac disease is an autoimmune disorder involving the small intestine caused by gluten consumption. In addition to the complications caused by the involvement of the digestive system, many autoimmune complications, especially autoimmune thyroid diseases, are also common in these patients. The aim of this study was to investigate the prevalence of autoimmune thyroid diseases in celiac children of Urmia for the optimal management of their treatment.

In this cross-sectional study, venous blood samples were taken from children living in Urmia whose celiac disease was previously confirmed. Anti-transglutaminase test (anti-tTG-) to confirm the diagnosis, Antithyroid Peroxidase antibody (anti-TPO) and Antithyroglobulin Antibody (anti-TG) tests to screen for autoimmune thyroid disease, and Thyroid stimulating hormone (TSH) test was performed to check thyroid function, all by ELISA method. Data were analyzed using descriptive statistics and chi-square test.

Seventy-seven patients with an average age of 11.84 participated in the study; 30 of them were male and 47 were female. Anti-TPO test was positive in 3.9% and Anti-TG test was positive in 11.7% of the patients, all of whom were female. The difference in both genders regarding Anti-TG was statistically significant (p=0.023). In most of the patients who had positive Anti-TPO, the Anti-TG test was also positive, and a significant positive correlation was seen between these two tests (R=0.82). In 26% of the patients, hypothyroidism and in 1.3% of them, hyperthyroidism was seen. Hypothyroidism was seen in celiac patients in whom Anti-TPO was positive, 66.7% and in those who Anti-TG was positive, hypothyroidism was seen in 45.5%, which were not statistically significant (p=0.12).

The results of this study showed that thyroid autoimmune diseases are high in celiac children, especially in females. Therefore, regular examination of thyroid function and monitoring autoantibodies in celiac patients seems necessary for optimal management of both conditions.

Occupational exposure of bakers to flour dust beyond the standard level leads to lung problems and allergies. The present study aimed to determine and compare the flour dust concentration in various bakeries in Urmia, Iran, in 2021.

In this study, three types of bakeries (Lavash, Barbari, and Sangak) with two baking methods (traditional and machine) were selected. The breathing air of 160 bakery workers was collected according to the MDHS 4/14 method by an IOM sampler at a flow rate of 2 liters per minute, and the flour dust was determined by the gravimetric method. Descriptive statistics and one-way analysis of variance were used to present and analyze data using SPSS statistical software (version 22).

The mean (±SD) for age and working experience of the bakers were 40.39 ± 10.17 and 15.13 ± 9.49, respectively. The concentration of flour dust in the air of all types of bakeries was higher than the occupational exposure limit (0.5 mg/m3). Traditional Lavash and machine Sangak bakeries had the highest (8.61 mg/m3) and the lowest (1.35 mg/m3) flour dust concentrations in the air, respectively. The traditional baking method produces more air pollution than the machine. The average concentration of flour dust in all types of bakeries showed a statistically significant difference (P<0.05).

The workers of different bakeries in Urmia are exposed to flour dust higher than the occupational exposure limit. Due to less contamination, baking bread with a machine is preferable to the traditional method. Lavash, Berberi, and Sangak bakeries are respectively the priority of occupational health control interventions. Educating workers regarding the effects of flour dust on health and proper working practices, application of general and local ventilation and health surveillance are suggested for all types of bakeries with the mentioned priority.

Respiratory complications arised from exposure to flour dust are among most common work-related diseases. This study was conducted to investigate flour dust exposure and its respiratory complications in bakery workers in Urmia in 2021.

This is a descriptive cross-sectional study conducted in 2021. The statistical population of the study consists of 166 bakers in Urmia. An standard questionnaire was used to assess the prevalence of respiratory symptoms in workers. Data analysis was performed at two levels of descriptive and analytical statistics by SPSS 22 software using t-test, chi-square and one-way ANOVA at the significant level of P <0.05.

The mean and standard deviation of age obtained 40.39 and 10.17 years and mean and standard deviation of work experience obtained 15.13 and 9.49 years. The most common complication in the studied people were chest sputum (22.3%) and cough (16.9%). According to the results, there were significant relationships between respiratory complications with the type of bakery (P= 0.0001), the type of contact with dust (P= 0.0001), and also with the type of job (P= 0.000).

The results of this study showed the existence of respiratory complications in the bakers of Urmia. Therefore, in order to reduce respiratory complications, it is necessary to do actions such as providing appropriate personal protective equipment, adequate ventilation, and reducing the duration of exposure.

 Ramadan fasting has numerous health benefits. Nevertheless, its beneficial role on liver function was evaluated by limited studies and different findings were recorded. So, this study aimed to define the effect of Ramadan Fasting on liver enzyme levels in Iranian healthy individuals.

Seventy healthy adult men without history of liver diseases and current usage of any medication affecting the liver were involved in the present survey. Anthropometric parameters and liver function tests were measured before and after Ramadan. The biochemical parameters including liver enzymes Aspartate aminotransferase (ALT), Alanine Aminotransferase (ALT) and Alkaline Phosphatase (ALP) were evaluated before and after Ramadan by the standard enzymatic method.

A decrease was observed in mean level of all liver enzymes. Indeed, the significant mean decrease in the ALT values in the post-Ramadan analyses was observed in the pre-Ramadan (p < 0.01). The values of ALP and AST decreased at the end of the study in the post-Ramadan group versus pre-Ramadan, but mean of the changes did not reach a significant level.

The changes in the concentrations of liver enzymes showed remarkable differences between groups. These data confirmed that Ramadan Fasting improved liver function in healthy adults and might be valuable in the management of liver function.

Escherichia coli is still the common host for ing and heterologous protein expression. Various strategies have been employed to increase protein expression in E. coli, but, it seems that external factors such as selection marker concentration can drastically affect the yield of protein and plasmid.
Alterations of protein expression and plasmid yields of E. coli in different concentrations of ampicillin, as selection marker, will be determined. In order to improve heterologous expression, the system will be redesigned and optimized.
The expression cassette of codon optimized EGFP for E. coli was synthesized in pUC57. The pUC57-GFP was transformed into E. coli Top10F’. The expression of GFP was verified by SDS-PAGE and flow cytometry after induction by IPTG (0.5 mM) and incubation with 0, 100, 200 and 300 µg.mL-1 ampicillin. Plasmid copy numbers of samples were determined by Real-Time PCR on AMP gene using regression line of diluted standard curve.
GFP expressing clones formed fair green colonies on LB agar supplemented with 0.5 mM IPTG and showed fluorescence in FL1 filter of flow cytometry and an extra protein band on SDS-PAGE gel. The fluorescent intensity of GFP in 0, 100, 200 and 300 µg.mL-1 ampicillin in medium were 549.83, 549.78, 1443.52, 684.87, and plasmid copy numbers were 6.07×109, 3.21×109, 2.32×1010 , 8.11×108, respectively. The plasmid yields were 55 ng.µL-1, 69 ng.µL-1, 164 ng.µL-1 and 41 ng.µL-1, respectively.
Protein and plasmid yields of E. coli are variable in different concentrations of ampicillin and need to be optimized in newly designed expression systems. Protein and plasmid yield in the optimized concentration (200 µg.mL-1) was significantly (p

Intermittent fasting during Ramadan entails major changes in metabolism and energy expenditure. This study sought to determine effect of the Ramadan fasting on serum levels of adiponectin and tumor necrosis factor-α (TNF-α) as two inter-related peptides involved in cells sensitivity to insulin and glucose metabolism. Total of seventy healthy men, with age range equal or greater than 30, with at least three type2 diabetes mellitus (DM) risk factors were selected. Serum lipid profile, anthropometric indices and plasma glucose levels were determined using conventional methods. Also, serum adiponectin and TNF- α concentra­tions were assessed using Enzyme-linked Immunosorbent Assay. Data were analyzed by paired t-test. Ramadan fasting resulted in a significant increase of serum adiponectin (P< 0.000), fasting glucose (P< 0.000) and triglycride (P< 0.001). Body mass index was lowered during the fasting (P< 0.000). Finally, no remarkable decrease was found in serum TNF-α levels (P= 0.100). Ramadan fasting resulted in augmented adipo­nectin levels which may help in improving metabolic stress induced by insulin resistance in men with predisposing factors of type2 DM.