فهرست مطالب sahar dashchi

Safflower (Carthamus tinctorius), as one of the most important economic products in the world, has one of the highest quality edible oils. Safflower cultivars are hardy plants compared to other plants due to different methods of tissue culture and regeneration. Therefore, it is necessary to provide a high efficiency method for the regeneration of safflower commercial cultivars. In this study, the effect of different concentrations of BAP, Kin and TDZ on callus induction and shoot regeneration of hypocotyl and cotyledon explants for two safflower cultivars (Soffeh and Golmehr) in different concentrations of BAP, Kin, TDZ and NAA (0, 0.2, 0.5 and 1 mg/l) were evaluated. The results showed that the highest frequency of shoot regeneration also was obtained on MS medium supplemented with 0 mg/l NAA and 1 mg/l TDZ (33%) from cotyledon explant of Golmehr cultivar. There was a significant difference between cultivar’s shooting, Golmehr cultivar showed a better shooting percentage. Finally, shoots were transferred to eight different rooting media. The best medium for rooting was MS medium supplemented with IBA (2 mg/l) + sucrose (2%) + agar (7%). The results showed that Golmehr cultivar was better than Soffeh cultivar in terms of regeneration and response to optimization.

In oilseed crops, a number of genes involved in the production of triacylglycerol have been identified that changes in their expression have increase the seed oil content. WRI1 and LPAAT are key genes in this synthetic pathway that their overexpression can increase the oil content. In this study, the expression vectors carrying WRI1 and LPAAT genes were designed and constructed for genetic transformation of tobacco (Nicotiana tabaccum) plants. The synthetic WRI1 and LPAAT genes were isolated from the PGH.WRI1 and PGH.LPAAT cloning vector using specific restriction enzymes and then cloned in the PGH.O3.2.2 intermediate vector under the control of SBP and Napin promoters and E9 terminator. Finally, the genetic cassettes were transferred to the plant transformation pBin19 binary vector. The resulting constructs were transferred to Agrobacterium tumefacience strain EHA105 and were used for genetic transformation of tobacco plants. Molecular analysis of transgenic plants confirmed the presence and activity of WRI1 and LPAAT genes. Seeds from transgenic plants were selected on the medium containing kanamycin and developed strong and healthy seedlings.