فهرست مطالب sayed kamal kazemitabar

In the present study, hairy root induction from leaf and stem explants in two Hyssopus species (H. officinalis and H. angustifilius) was assessed using four Agrobacterium rhizogenes strains including A4, ATCC15834, LB9404 and 2656 on three culture media (MS, ½ MS, B5) as a factorial experimental based on a completely randomized design with three replicates. Almost all leaf and stem explants produced hairy roots. A fragment of transferred rol B was amplified through PCR from the genomic DNA extracted from transformed roots. Different A. rhizogenes strains and culture media and Hyssopus species had significant effects on hairy root percentage and root length. The highest rooting percentage (%71) and root length (4.7 cm) were resulted from stem explants of H. angustifolius co-cultivated with the strain ATCC15834 on MS culture medium. While, the leaf explantsof H. officinalis co-cultivated with the strain A4 on MS culture medium showed the rooting percentage of 55% and the root length of 1.6 cm at most. The MS medium was the best culture medium for both species. These results can be useful in genetic manipulation of hyssop and its hairy root culture to produce high-value secondary metabolites.

Recently, transient gene expression has been developed to provide a more rapid means of assessing plant tissues as a protein production platform without the labor-intensive and time-consuming process of generating stably transformed transgenic plants. This study reports the expression of HDA19 gene in two species of tobacco plants (Nicotiana tabacum and Nicotiana bentamiana) by means of transient transformation. Specific primers were designed and used for PCR amplification and cloning of HDA19 gene in the plant expression vector pB2GW7. The recombinant construct was transferred into Agrobacterium tumefaciens strain GV3101, and was used for Agrobacterium mediated transformation of tobacco plants. The presence of the desired gene in transgenic lines was confirmed through colony PCR. The expression of the protein in transgenic lines was confirmed by immune-dot blot assay and ELISA. Although the transformation of the two species was confirmed by immune-dot blot assay and SDS-PAGE, recombinant protein production in Nicotiana tabacum plants was confirmed by ELISA and it was estimated 400 µg per gram wet weight of tobacco leaves. According to the results, this species is the appropriate host for the production of recombinant HDA19, one of the histone deacetylases, rather than Nicotiana bentamiana.

In recent years، certain strains of Florescent Pseudomonas resident in rhizosphere have considerably attracted lots of attention due to their protection role against various types of plant pathogens. The ability of antibacterial metabolites production is one of the important characteristics of these bacteria that plays a key role in plant production against pathogens. Fluorescent pseudomonades are able to produce antibiotics playing a role in the biological control of plant pathogens. 2،4 – diacetylflourogolocinol (DAPG)، is one of the most significant antibiotics controlling soil borne plant diseases which is produced by strains of Pseudomonas fluorescent. The Production of the antibiotic is controlled by six genes in three transcription units. In this study، four genes: phlA، phlC، phlB، and phlD that have direct role in the synthesis of the antibiotic and have been transcribed in one unit and organized in one operon were isolated from Pseudomonas fluorescens genome using PCR. Eventually، Carrying out the validation of the isolation using the Nested PCR technique، the operon was cloned in the pGEMT-easy vector system II for the future studies.