vahid roshan

Persian sage (Salvia mirzayanii L.) is a medicinal plant belonging to the mint family, which has been of interest since ancient times due to its many medicinal properties. In order to investigate the effect of different concentrations of amino acids (0, 0.5, 1 and 1.5 gr/L) and storage duration (0, 2, 4 and 6 months) on the amount and changes of Iranian sage essential oil, a factorial experiment was conducted in a randomized complete block design. Amino acid foliar application had a positive effect on the measured vegetative and reproductive traits of Iranian sage plant as well as the percentage of compounds after harvest. In this study, the most important compounds found in Iranian sage essential oil after harvesting were 5-neo-sedranol (20.11%), Lnalyl acetate (16.52%) and alphaterpinyl acetate (14.28%). The content of total phenol increased by increasing the level of amino acid foliar application up to the level of 1 mg/L (82.6 mg/L) and after that it decreased, and by continuing storage for 6 months after harvesting, it increased by 23.15% compared to four months. The highest antioxidant activity was obtained from foliar spraying of amino acid with a concentration of 1 mg/L, and storage for 6 months after harvest reduced it by 29% compared to the time of harvest. Based on the results of this study, it is recommended to use amino acid up to a concentration of 0.5 gr/L in order to achieve the maximum fresh and dry weight of the plant.

The use of humic acid fertilizer had a positive effect on the measured characteristics of Iranian sage and also the percentage of essential oil compounds. Dry weight of leaves (0.5 g) and number of inflorescences (35) and inflorescence weight (1.8 g) due to the use of 15 g of humic application was obtained. The amount and type of essential oil constituents were determined by GC and MS/GC devices and a total of 62 compounds were isolated from Iranian sage essential oil under the influence of experimental treatments, of which 18 similar compounds were present in all treatments. The most important compounds in Iranian sage essential oil was reported 5-neo-Cedranol (20.11%), Linalyl acetate (16.52), Terpinyl acetate (14.28%), Linalool (5.22) and Bicyclogermacrene (4.68%). Compounds Percentage increased with increasing humic acid density. As a result of using a concentration of 15 g / l of humic acid, the 5-neo-Cedranol, α-Terpinyl acetate, Bicyclogermacrene and Linalyl acetate compounds increased by 37.5, 22.5, 32.7 and 20.5 percent compared to control. The total phenolic compounds in the plant extract due to the use of 15 g / l humic acid increased significantly and by 72.8% compared to the control without fertilizer (516.43 mg / l). The highest phenolic composition in Iranian sage extract in this study was Rosmarinic acid at the rate of 421.64 mg / l at the level of 15 g / l humic acid. The use of humic acid caused a significant increase in the amount of antioxidants in the plant extract.

Recently, there has been growing interest in research concerning the possible use of plant extracts as alternatives to synthetic insecticides. Essential oils are among the best-known substances tested against insects. These compounds may act as fumigants, contact insecticides, repllants, antifeedants and may affect growth and development of the insect. In an attempt to find a natural and inexpensive method for the control of stored-product pests, contact toxicity of essential oils of Mentha longifolia L., Tagetes minuta L. and Salvia macrosiphon Boiss. was investigated on adult insects of Sitophilus oryzae L. (Coleoptera, Curculionidae) on laboratory condition. The oils were extracted by using Clevenger apparatus. The experiment was conducted using a completely randomized design of factorial experiment with five concentrations and three replications at R.H in darkness. The results showed that the percentage of mortality was increased with increase in concentration and exposure of time. The highest contact toxicity was related to M. longifolia and the lowest toxicity was recorded for S. macrosiphon against insects. At the highest concentration of essential oils (35 µl/ml) by M. longifolia,T. minuta and S. macrosiphon the mortality were recorded as 93.3%,83.3% and 70% against S. oryzae after 48h, respectively. The LC50 values were evaluated by M. longifolia, T. minuta and S. macrosiphon 18.892, 21.003 and 24.482 for S. oryzae after 48h. According to the results, the essential oils of M. longifolia,T. minuta and S. macrosiphon are sources of biologically active vapor that can be used in protection of stored grains against rice weevil.