Effect of humic acid application on growth characteristics and changes in the active ingredients of medicinal plant

The use of humic acid fertilizer had a positive effect on the measured characteristics of Iranian sage and also the percentage of essential oil compounds. Dry weight of leaves (0.5 g) and number of inflorescences (35) and inflorescence weight (1.8 g) due to the use of 15 g of humic application was obtained. The amount and type of essential oil constituents were determined by GC and MS/GC devices and a total of 62 compounds were isolated from Iranian sage essential oil under the influence of experimental treatments, of which 18 similar compounds were present in all treatments. The most important compounds in Iranian sage essential oil was reported 5-neo-Cedranol (20.11%), Linalyl acetate (16.52), Terpinyl acetate (14.28%), Linalool (5.22) and Bicyclogermacrene (4.68%). Compounds Percentage increased with increasing humic acid density. As a result of using a concentration of 15 g / l of humic acid, the 5-neo-Cedranol, α-Terpinyl acetate, Bicyclogermacrene and Linalyl acetate compounds increased by 37.5, 22.5, 32.7 and 20.5 percent compared to control. The total phenolic compounds in the plant extract due to the use of 15 g / l humic acid increased significantly and by 72.8% compared to the control without fertilizer (516.43 mg / l). The highest phenolic composition in Iranian sage extract in this study was Rosmarinic acid at the rate of 421.64 mg / l at the level of 15 g / l humic acid. The use of humic acid caused a significant increase in the amount of antioxidants in the plant extract.