z valiei

Timely diagnosis of brucellosis is the starting point for effective programs to control brucellosis in humans and animals.

This study aimed to detect Brucella infection in milk samples from livestock in Famenin, an endemic region of western Iran, using a milk ring test and molecular techniques.

In this cross-sectional study, 738 raw milk samples were randomly collected from cattle, sheep, and goats. Milk samples were screened using the milk ring test (MRT). In addition, polymerase chain reaction (PCR) method was applied to detect Brucella spp. in all MRT-positive samples. DNA from the milk samples was extracted and used for PCR using the BCSP31 and IS711 loci.

Of the samples tested using MRT, 46 (6.23%, 95% CI: 2.83-9.63%) yielded positive results. Of the 46 seropositive samples, 42 (91.30%) were from sheep and 4 (8.70%) were from goats, while no bovine samples had positive MRT results. PCR analysis confirmed that 78.26% (36/46) of MRT-positive samples belonged to the genus Brucella. Furthermore, 83.33% (30/36) of the confirmed samples were identified as B. melitensis, while 16.66% (6/36) were identified as B. abortus.

The results obtained from MRT evaluation of milk samples did not align entirely with the findings of the molecular examinations. The PCR method has minimal biological contamination and high sensitivity and accuracy, especially for determining Brucella species. Raw milk should be routinely assessed for Brucella contamination. This work is necessary to identify hidden infections and break the chain of transmission of brucellosis.

This study aimed to investigate the relationship between family function and cognitive regulation of emotion with psychological well-being in high school students in Marand City. The population of the study were all high school female students (N=480) from whom 215 students were randomly selected and participated in the study. The questionnaires used to collect the data included family function questionnaire (Epstein, Baldwin, and Bishop), cognitive emotion regulation questionnaire (Garnefsky, Craij, and Spinhophen) and psychological well-being (Reif). The present study was a correlational study and the data analysis was performed using simple correlation and regression. The correlation results showed that there was a positive and significant relationship between the family function variable and psychological well-being and a positive and significant relationship between the variable of cognitive emotion regulation and psychological well-being (p ˂ .05). The regression coefficient results also indicated that the family function variable and the cognitive adjustment variable can provide psychological well-being in female students with positive and meaningful prediction. Therefore, it can be said that with increasing family function and also with increasing cognitive emotion regulation in female students, their psychological well-being also increases.