zahra zafari

The aim of this study was the evaluation of effectiveness of education based on problem solving approach on exam anxiety of medical students of Islamic Azad University, Tehran Medical Sciences Branch.

This research is quasi-experimental research in terms of applied purpose. The statistical population of this study was all 5928 medical students of Islamic Azad University, Tehran Branch of Medical Sciences, of which 60 were divided into experimental and control groups. The intervention group received training based on a problem-solving approach for 10 sessions. Data were collected using Sarason's exam anxiety (1957) Questionnaire and analyzed using SPSS version 26 by ANCOVA statistical test.

In the pre-test stage, there was no significant difference in the mean test anxiety between the two groups. A significant difference was observed in the mean anxiety score and its dimensions of intervention group after the intervention (p=0.05). It means that the teaching model based on the problem-solving approach has a significant effect on the exam anxiety among medical students of the Islamic Azad University of Tehran Medical Sciences Branch.

Education based on the problem-solving approach is one of the important factors that plays a role in reducing students' exam anxiety.

The B-cell-specific Moloney murine leukemia virus integration site1 (BMI-1) is one of the famous members of the Polycomb ring finger group, which plays a crucial role in the gene transcription regulation through histone changes. Hence, it is believed to be necessary to further clarify the effects of the BMI-1 clinical.

This cross-sectional study was conducted on 70 acute myeloid leukemia (AML), 70 chronic myeloid leukemia (CML), and 20 healthy individuals, as the control group. We used real-time quantitative polymerase chain reaction in order to assess the BMI-1 level expression and its effect on prognosis in AML patients in the Molecular Pathology Research Center.

The results of the present work indicated that the BMI-1 overexpression was significantly higher in the AML and CML patients compared with that in the healthy controls (P < 0.001). Furthermore, a significant relationship was observed between the BMI-1 overexpression and poor prognosis in the AML patients (Hazard ratio=1.749, P < 0.001, 95% confidence interval = 1.31-2.32). Additionally, BMI- 1high was found in chronic and blastic phase in the CML patients (P < 0.001).

We concluded that investigation of BMI-1 gene expression pattern will be conducive to the prognosis and treatment of myeloid leukemia.

The purpose of this study was to evaluate the effectiveness of education model based on problem solving approach on educational engagement of medical students of Islamic Azad University, Tehran Medical Sciences Branch.  

This research is quasi-experimental research in terms of applied purpose. The statistical population of this study was all 5928 medical students of Islamic Azad University, Tehran Branch of Medical Sciences, of which 60 were divided into experimental and control groups. The intervention group received training based on a problem-solving approach for 8 sessions. Data were collected using Zerang Academic Involvement Questionnaire (2012) and analyzed using SPSS version 25 by MANCOVA statistical test.

In the pre-test stage, the mean of academic involvement was not significantly different between the two groups. Comparison of academic involvement in the intervention group showed that a significant difference was observed in the mean of this variable and its dimensions after the intervention (p= 0.05). It shows that the model of education based on the problem-solving approach has a significant effect on the academic engagement of medical students of Islamic Azad University, Tehran Branch of Medical Sciences.

Education based on problem solving approach is one of the important factors that plays a role in students' academic engagement.

Long QT syndrome (LQTS) is characterized by the prolongation of QT interval, which results in syncope and sudden cardiac death in young people. KCNQ1 is the most common gene responsible for this syndrome. Molecular investigation was performed by DNA Sanger sequencing in Iranian families with a history of syncope. In silico examinations were performed for predicting the pathogenicity of the novel variant. A novel homozygous KCNQ1 frameshift mutation, c.1426_1429delATGC (M476Pfs*4), was identified, and then the current literatures of five patients were reviewed regarding the LQTS. The novel frameshift mutation has been reported for the first time among the Iranian population. Our finding along with the case series study of LQTS patients illustrates the importance of genetic and case series in precise detection of the frequency of LQTS carriers.

Erythroblast transformation specific Related Gene (ERG) is a member of ETS transcription factors family, which act as a regulator of primary hematopoietic cell differentiation. Previous researches suggested proto-oncogene for response to mitogenic signal of different cell with MAP kinase and help to tumorgenesis, apoptotic and prognostic role for ERG gene in acute myeloid leukemia (AML) patients. The aim of his study was to evaluate ERG gene expression level in AML cases compare to control group.

For analysis of gene expression, total RNA was isolated from bone marrow (BM) and peripheral blood of AML `s patient. This study was conducted on 54 AML patients and 54 healthy individuals as control group. ERG gene expression and reference gene glucose-6-phosphate isomerase (GPI) was assessed by using real-time polymerase chain reaction (RT-PCR) method. Clinical and laboratory findings studied cases were extracted from medical documents.

This study results indicated that the level of ERG gene expression in AML patients had no significant differences compare to control group (1.81±2.41) (P <0.05), Also ERG gene expression was correlated to patients hematocrit (P <0.05).

This research recommends that there were no significant relation between ERG expression and control groups. It seems that ERG gene assessment is not a good index for AML patient’s evaluation.

Considering the interactions between depression and anxiety on the increase in blood glucose in diabetic patients and the need for intervention to improve these variables, the aim of this study was to investigate the effectiveness of Lazarus multimodal therapy on reducing the symptoms of depression, anxiety and glycemic control in women with type 2 diabetes.
In this study, which was performed on women with type 2 diabetes with high score in depression and anxiety and glycosylated hemoglobin (HbA1C) level more than 7%, 24 subjects who entered the study were selected by convenient method and they were randomly assigned into two groups of experimental and control (each group included 12 subjects). Research instrument include Beck Depression Inventory-II, Beck Anxiety Inventory and glycosylated hemoglobin. The experimental group was trained at Lazarus multimodal psychotherapy group workshop for three months (12 weekly ninety-minute sessions) in the spring and summer of 2016 at Samen Health Center- Mashhad. The control group was in the waiting list. Also, multivariate analysis of covariance analysis was used to analyze the data through SPSS software.
The results of this study showed that Lazarus multidimodal therapy significantly (P=0.001) reduced the symptoms of depression, anxiety and decreased blood glucose levels in women with type 2 diabetes compared to control group.
Totally based on the findings, Lazarus multidimodal therapy can be effective to reduce the symptoms of anxiety, depression and physical symptoms among diabetic patients.

Jervell and Lange–Nielsen syndrome is an autosomal recessive disorder caused by mutations in KCNQ1 or KCNE1 genes. The disease is characterized by sensorineural hearing loss and long QT syndrome.
Here we present a 3.5-year-old female patient, an offspring of consanguineous marriage, who had a history of recurrent syncope and congenital sensorineural deafness. The patient and the family members were screened for mutations in KCNQ1 gene by linkage analysis and DNA sequencing.
DNA sequencing showed a c.1532_1534delG (p. A512Pfs*81) mutation in the KCNQ1 gene in homozygous form. The results of short tandem repeat (STR) markers showed that the disease in the family is linked to the KCNQ1 gene. The mutation was confirmed in the parents in heterozygous form.
This is the first report of this variant in KCNQ1 gene in an Iranian family. The data of this study could be used for early diagnosis of the condition in the family and genetic counseling.

Graphene modified glassy carbon electrodes have been developed for the determination of calcium dobesilate in pharmaceutical formulations. The prepared graphene and CNTs were used as a conductive substrate for electrochemical study of calcium dobesilate. The direct electrochemistry of calcium dobesilate showed a reversible cyclic voltammogram with a formal potential of 133 mV (vs. Ag/AgCl) for graphene modified electrodes in 0.1 M phosphate buffer. The linear concentration range of the sensor is 30-120 ngr for graphene modified electrode, respectively. The lifetime of biosensor is more than 2 weeks. The proposed modified electrode provides a new promising and alternative way to detect calcium dobesilate.

Infrastructure of increased productivity in universities requires emphasis on improving the quality of its faculty members and don’t updating of peoples in their profession, according to the quality of the course of advanced teaching methods. The present paper was performed aiming at content analysis of in service training curriculum in faculties of Islamic Azad University, Tehran Medical Branch, and comparing it with the approved topics.

In the descriptive survey research, faculty members and specialists of educational sciences group were studied. Sample size was calculated as 86 subjects by Morgan table and they were chosen by systematic random sampling. Data collection was done by author's questionnaire for which the stability was verified by Cronbach alpha of 0.839. One sample t test was used to analyze the data.

Correspondence of in service training of advanced teaching method was desirable according to approved topics of Islamic Azad University, Tehran Medical branch. According to participants’ view, each component of curriculum, including objectives, method, contents, and evaluation was significant and suitable. Also, the compliance of the elements of objectives, contents, method and evaluation in the curriculum of advanced teaching method were suitable and significant.

In order to increase quality and empowerment of faculty members, the content of courses in service training should be continually adapted by planners with the approved topics.

DNA Profiling has become one of the most robust and reliable methods at forensic identification; However, an insufficient DNA quantity (less than 100 Pg or 33 copies) found often in forensic evidence samples, is a major hindrance. Amplification of such low copy number DNA samples is attainable with the most efficient whole genome amplification (WGA) method, named improved primer extension preamplification (I-PEP) PCR.

By initial assessment of existing PEP and I-PEP methods on serially diluted DNA, it was attempted to reach an improved method leading to reliable profiling with the lowest amount of template. This method employs degenerate 15-mer PCR primers followed by specific amplification of DNA with specific primers.

Subsequent to the amplification with the new modified and improved I-PEP, which we term KI-PEP PCR, complete DNA profile was obtained from only 2.5 pg of input DNA. Using this method, a fragment size of 1106 bp was effortlessly amplified with the specific primers.

The Utility of KI-PEP PCR not only increases the low quantity of DNA, but also provides the optimum length appropriate to DNA Typing techniques.