فهرست مطالب zhaleh mohsenifar

Nowadays occurrence of resistant to clarithromycin which is a key component of eradication therapy for Helicobacter pylori strains is a worldwide health concern. Point mutations in 23srRNA gene is one of the main causes of clarithromycin resistance. This study aimed to examine rate of clarithromycin resistance and its link with 23srRNA gene point mutation.

This study was conducted on 100 patients suffering from gastric complications had referred to tehran valiasr clinic during january of 2022. Two separate biopsy samples were collected from each patient and used for pathological and microbiological examinations. Antimicrobial susceptibility tests were performed by agar dilution and PCR.

53% (53/100) of the study patients were diagnosed as H. pylori+. Pathological findings indicated that 54.7% (29/53) of the H.pylori+ patients suffered from Chronic Gastritis, 37.7% (20/53) from Sever Active Gastritis and 7.5% (4/53) with Intestinal Metaplasia. Clarithromycin resistant were found among 13.2% (7/53) of patients. The MIC values of 0.125 mg/L and 2 mg/L were determined as the MIC50 and MIC90 values, respectively. Based on PCR results, all clarithromycin resistant strains had point mutations A2142G in 23SrRNA gene.

The findings revealed the existence of point mutations in 23srRNA gene which may be related with resistance to clarithromycin in H. pylori strainsKeywords: Helicobacter pylori, Clarithromycin Resistance, Histopathological Changes, Minimum Inhibitory Concentration

Long bone segmental deficiencies are challenging complications to treat. Hereby, the effects of the scaffold derived from the human demineralized bone matrix (hDBMS) plus human adipose stem cells (hADSs) plus photobiomodulation (PBM) (in vitro and or in vivo) on the catabolic step of femoral bone repair in rats with critical size femoral defects (CDFDs) were evaluated with stereology and high stress load (HSL) assessment methods.

hADSs were exposed to PBM in vitro; then, the mixed influences of hDBMS + hADS + PBM on CSFDs were evaluated. CSFDs were made on both femurs; then hDBMSs were engrafted into both CSFDs of all rats. There were 6 groups (G)s: G1 was the control; in G2 (hADS), hADSs only were engrafted into hDBMS of CSFD; in G3 (PBM) only PBM therapy for CSFD was provided; in G4 (hADS + PBM in vivo), seeded hADSs on hDBMS of CSFDs were radiated with a laser in vivo; in G5 (hADSs + PBM under in vitro condition), hADSs in a culture system were radiated with a laser, then transferred on hDBMS of CSFDs; and in G6 (hADS + PBM in conditions of in vivo and in vitro), laser-exposed hADSs were transplanted on hDBMS of CSFDs, and then CSFDs were exposed to a laser in vivo.

Groups 4, 5, and 6 meaningfully improved HSLs of CSFD in comparison with groups 3, 1, and 2 (all, P = 0.001). HSL of G5 was significantly more than G4 and G6 (both, P = 0.000). Gs 6 and 4 significantly increased new bone volumes of CSFD compared to Gs 2 (all, P = 0.000) and 1 (P = 0.001 & P = 0.003 respectively). HSL of G 1 was significantly lower than G5 (P = 0.026).

HSLs of CSFD in rats that received treatments of hDBMS plus hADS plus PBM were significantly higher than treatments with hADS and PBM alone and control groups.

Lead, as the most important toxic heavy element, has several devastating effects on human health and influences most biochemical and physiological functions. It is widely accepted that lead can adversely affect the cardiovascular system since it can be quickly absorbed and recycled in the blood strain.

This survey scrutinized the effects of N-acetylcysteine (NAC) on the oxidative damage, inflammation, and expression of protein kinase C-alpha (PKC-α) and ankyrin repeat domain 1 (ANKRD1) genes in the heart tissue of rats exposed to lead (Pb).

The rats were incidentally divided into five groups, including four study groups for the investigation of the effects of the single and continuous doses of lead were examined with and without NAC and a control group (G1). The levels of malondialdehyde (MDA), total antioxidant capacity (TAC), interleukin (IL)-10, and tumor necrosis factor alpha (TNF-α) were analyzed. A reverse transcription polymerasechain reaction was applied to investigate the expression of PKC-α and ANKRD1 genes.

Continuous exposure to Pb significantly decreased serum levels of TAC and IL-10; however, it increased MDA and TNF-α contents (P<0.001). The continuous dose of Pb also dramatically increased the expression of PKC-α and ANKRD1 genes in the cardiac tissue by 4.27-fold and 3.07-fold, respectively (P<0.001). N-acetylcysteine treatments not only improved morphological changes, oxidative stress, and inflammatory biomarkers but also compensated antioxidant capacity and the expression of PKC-α and ANKRD1 genes in cardiac tissues.

Lead exposure is remarkably related to cardiotoxicity mainly by inducing oxidative stress, inflammation, and antioxidant discharge. N-acetylcysteine ameliorates Pb-induced cardiotoxicity by improving the antioxidants capacity, mitigating oxidative stress, and down expressing PKC-α and ANKRD1 genes.

Frozen section is mainly performed to determine malignity, so it is of fundamental importance to evaluate the diagnostic accuracy of frozen sections performed for each group of tissues in order toreduce false diagnoses, unnecessary surgeries and followingunwanted complications. This study is conducted to evaluate the diagnosticaccuracy of frozen section performed on ovary tissue compared to permanent sections.

In this retrospective study, we evaluate frozen sections performed at Taleghani hospital pathology department in five yearsandtheir results were compared with permanent sections’ results to calculate sensitivity, specificity, negative predictive value and positive predictive value. In cases which results were discordant,we check for probable reasons.

83species were evaluatedfrom patients with a mean age of 42.9 years; the smallest was 19 andthe oldest was 86 years old(Std. dev: 12.84). 75cases of frozen sections were benign(90.4%) and8 cases were malignant. 73cases of permanent sectionswere benign(88%) and10 cases were malignant(12%. Sensitivity, specificity, positive predictive value and negative predictive value rates were 66%, 100%, 100% and 96.55% respectively.

This study shows the importanceof cooperation between pathologist and surgeons that they can prevent unnecessary surgeries. Inour study the only cases of discordancy were borderline tumorsandthe diagnostic accuracy for all other species were perfect.

Autologous cartilage graft in rhinoplasty is associated with common challenges, such as resorption, inflammation, and bone metaplasia. This study aimed to assess platelet-rich fibrin's long-term effect, as a superphysiological source of biomolecules, on cartilage regeneration in an animal model.

Cartilage and fascia were excised autologously from the ear and leg of thirty-three Wistar rats. They then transplanted with cartilage alone (control group), cartilage wrapped with fascia (fascia group), and cartilage wrapped with PRF (fibrin group) autologously and subcutaneously.

The histological results demonstrated no significant difference in chondrocyte viability, fibrosis, and resorption between three groups after six months. However, other parameters, including calcification, bone metaplasia, infection, and basophilia, were lower in the fibrin group.

This study concluded that PRF could be an appropriate autologous cell source for cartilage regeneration and nasal augmentation in the long term.

Odontogenic lesions range from simple cysts to benign tumors and carcinomas. Unicystic ameloblastoma is a monocystic lesion and a less aggressive tumor compared to multicystic ameloblastoma. Ameloblastic carcinoma is the malignant variant of the multicystic ameloblastoma and may arise de novo or from malignant transformation of a long-standing multicystic ameloblastoma.
We collected 54 tissue samples obtained from patients from 2000-2017 that were stored in the archives section of the Pathology Department of Taleghani Educational Hospital, Tehran, Iran. The specimens were processed for immunohistochemistry analysis. Immunostaining of the markers was assessed via quantitative methods. Statistical analysis was performed using one-way ANOVA and the chisquare test.
One-way ANOVA analysis and the chi-square test did not reveal any statistically significant differences between the expression levels of endocan, ET-1, and ETAR and lesion type. A positive correlation existed between ET-1 and ETAR expression levels in unicystic ameloblastoma and multicystic ameloblastoma (Pearson’s r = 0.506, P<0.002), and between ET-1 and ETAR expression levels in unicystic ameloblastoma and ameloblastic carcinoma (Pearson’s r = 0.376, P<0.024).
This study revealed a positive correlation between the histological degree of lesion and endocan, ET-1, and ETAR expression levels. Hence, it might suggest that multicystic ameloblastoma develops from unicystic ameloblastoma. Over time, multicystic ameloblastoma may undergo a malignant transformation to ameloblastic carcinoma. Possibly, a simple cystic neoplasm can progress to a cystic lesion with invasion to the connective tissue wall and a gradual development into a true neoplasm (multicystic ameloblastoma), with potential for malignant transformation (ameloblastic carcinoma). Endocan, ET-1, and ETAR can be used as prognostic biomarkers for different variants of multicystic ameloblastoma and possible new targets for cancer therapy.

Diabetes is a metabolic chronic disorder that makes it difficult to heal wounds because of its complications. To this point, many studies on the use of mesenchymal stem cells in wound healing process particularly diabetic ulcers have been done. In this study, we evaluate the impact of mesenchymal stem cells-derived conditioned medium (MSC-CM) on diabetic wound strength. The type of study is experimental. 24 rats were divided into four groups: normal control, diabetic control, diabetic placebo (culture medium treated) and diabetic experimental (treated with MSC-CM). Diabetes mellitus was induced in the diabetic rat groups by intraperitoneal injections of alloxan. One full-thickness skin incision was made on the dorsal region of each rat.Conditioned medium were obtained from human bone marrow mesenchymal stem cells. 24 hours after wounding, diabetic experimental rats received 200 µl of conditioned medium, and diabetic placebo rats received 200 µl of of culture medium intravenously. 15 days after wounding, the wounds were sampled and biomechanical testing of elasticity (tensiometery) was performed on them and strength of wounds was evaluated by ANOVA test. Body weight and blood glucose difference of diabetic rats was significantly higher than the control group (P=0.00). Maximum force of experimental group was 3.45±1.05 while this criterion in diabetic control was 1.76± 0.56 and in diabetic placebo was 2.30±0.62, which was significantly increased (p<0.05). Morover,the maximum stress of experimental group was 0.34±0.1, which compared to the diabetic control group (0.18±0.05) showed a significant increase. In addition, standard elastic stiffness of the experimental group was 3.19 ±1.07, whichcompared with diabetic placebo group (2.2 ±0.19) significantly increased. It seems to be human mesenchymal stem cells-derived conditioned medium has significant effect on wound strength in diabetic rats.

Lymphoepithelial cyst of oral cavity is a rare cystic lesion that presents as an asymptomatic submucosal mass and is usually discovered during routine dental examinations. The site most commonly affected is floor of the mouth but tonsillar involvement seems to be very rare. Multiple lymphoepithelial cysts have been reported in parotid of HIV positive patients however in oral cavity they usually present as solitary lesions. We report a case of multiple bilateral lymphoepithelial cysts of the palatine tonsils in a 72 years old male that presents with fever, pain and obstructive sleep apnea. We did not find any bilateral or multiple tonsillar lymphoepiyhelial cysts in the literature and the present paper seems to be the first report. We suggest although lymphoepithelial cysts rarely occur in the tonsils but they should be considered in differential diagnosis of adenotonsillar enlargement and related obstructive sleep apnea.

The aim of this study was to comprehensively analyze histopathologic parameters of Whipple pancreaticoduodenectomy specimens at Taleghani general hospital pathology department. The Whipple procedure is performed for variety of tumors involving the head of the pancreas, ampulla of Vater, common bile duct, or duodenum.Patients and Records of all cases of Whipple pancreaticoduodenectomy between 2007 and 2011were retrospectively reviewed and pathological details of diagnosis and staging were extracted. A total of 51 patients underwent Whipple procedure during a 5-year period, including 37 males and 14 females. The average age was 57 years (18-82 years). The most frequent presenting symptoms were jaundice and weight loss. Forty-four patients (86.3%) had malignant and 7 (13.7%) had benign lesions. Among malignant lesions, 27 (61.4%) were ampullary carcinomas, 12 (27.3%) were pancreatic carcinomas and 5 (11.4%) were cholangiocarcinomas. The pathological stage of most of the tumors was T3 (50%); followed by T2 (29.5%), and T1 (15.9%); only 4.5% were T4. Mean tumor size was 2.8 cm (0.2-7 cm). Duodenal and common bile duct margins were tumor-free in most cases (95.5 %). The pancreatic margin was free in 81.8% of patients; this margin had not been evaluated in 5 patients. Nearly 38.6% of all tumors showed vascular invasion while 68.2% showed perineural invasion. The average number of dissected lymph nodes was 4 (range 1-15); although in 25% of specimens, no lymph nodes had been found. Twelve specimens (35.3%) had lymph node metastases. The present study demonstrates that most of our patients are diagnosed with malignancy, at advanced stage, and further research is needed to develop practical methods for earlier diagnosis. The fact that 25% of specimens had no lymph nodes needs more consideration.

Ossifying fibroma (OF) is a benign fibro-osseous lesion which was first described by Menzel in 1872. It is commonly seen in the head and neck regions and represents an aggressive pattern when the midface and paranasal sinuses are involved. We report a 36 years old white woman with ossifying fibroma in the right ethmoid sinus. Computed Tomography (CT) scan images showed a hyperdense mass. Transnasal endoscopic resection was performed and histopathological examination confirmed the diagnosis of ossifying fibroma. The present case is notable because involvement of the ethmoid sinus is rare in this condition with only 48 cases reported in the literature until June 2011.

A 33-year-old female presented with intermittent abdominal pain and severe attacks of lower gastrointestinal (GI) bleeding since 2 weeks. She has had history of irregular menses in last 3 months. In the labeled RBC-Scan study, the bleeding site was detected in left upper quadrant (LUQ). In surgery, a tumor with the diameter of 2×1cm in a 65 cm distance of ligament of Trietz was detected. In pathological assessment jejunal metstatic choriocarcinoma was reported. Generally, choriocarcinoma is very rare among the intestinal metastatic tumors, including less than 5% of these tumors. Its presentation is acute lower GI bleeding and organ rupture, especially splenic rupture. These tumors will respond appropriately to the treatment in initial stages. Chemotherapy is the treatment of choice in these tumors.