جستجوی مقالات مرتبط با کلیدواژه « ریبوزوم » در نشریات گروه « علوم پایه »

MicroRNAs (miRNAs) are small, non-coding, and regulatory molecules that play an important role in post gene expression regulation in response to biotic and abiotic stresses. Although Canola (Brassica napus) is a globally important oilseed, little is known about its regulation mechanisms target genes of drought-responsive miRNAs. Then, in this study drought-responsive miRNAs target genes of bna-miR156b, bna-miR156c, bna-miR156g, bna-miR171a, bna-miR171d, bna-miR171e, bna-miR172d, bna-miR399a, bna-miR399b, bna-miR396a, bna-miR395d, bna-miR395e and bna-miR395f in Canola were identified by bioinformatics tools. Molecular function, biological process, cellular component, proteins interaction, and relation of their pathways were investigated. In the present study were identified 225 target genes for miRNAs. Bioinformatics study showed that ribosome, proteasome, and chaperon-related genes of RPP1C, RPL36AA, RPL9D, RPS11, RPT1A, RPT2a, RPT4A, RPN11, HSF1, HSFA1E, HSF4, and HSFB2B are targets of drought-responsive miRNAs of bna-miR172d ،bna-miR156b/c/g، bna-miR860، bna-miR396a and bna-miR395d. Ribosomes, proteasomes, and chaperons are regulated by drought-responsive miRNAs for protein regulation and stress tolerance under drought conditions. Laboratory study of identified genes in this study can be a fundamental step in the production of drought-resistant Canola cultivars in inbreeding and genetic engineering programs.

Microalgae are rich sources of active metabolites, which today are considered as important sources of biofuels in addition to medicinal and food uses. In this study, ribosomal markers were used to determine the phylogenetic posation of microalgae species which isolated from the southern coast of the Caspian Sea.

For molecular studies, microalgae grown in suspension culture medium were precipitated using iron (III) chloride solution. After extraction of genomic DNA by freez- thawing method, the gene loci encoding ribosomal subunits (16srDNA) were amplified using 36 pairs oligoes by polymerase chain reaction (PCR), the amplified gene loci were inserted into the pTZ57R /T vector by TA cloning technique then transformed in the Dh5α bacterial and sequenced follow by purification.

Fourteen pairs of primers showed acceptable amplification of target gene in the microalgae genome. The obtained sequences were edited by Vector NTi ver. 11 software and then blasted at the NCBI. Sequences with more than 90% similarity were selected and the molecular distance of the data was estimated by MEGA 6 software and phylogenetic analyzes were performed by calculating the maximum short molecular trees (Maximum Parsimony, MP) for the sequence of the studied genes, and Then, Bootstrap 1000 index was used for analysis to test the accuracy of the nodes.

The results of phylogenetic tree and similarity matrix with Maximum Parsimony method for all 14 gene regions showed that the studied species has the lowest genetic distance with the algal strain Spirulina laxissima.