جستجوی مقالات مرتبط با کلیدواژه « ریشه زایی » در نشریات گروه « زیست شناسی »

After pines, Ors (Juniperus) is the second most diverse genus of Coniferals, consisting of approximately 75 species and comprising the majority of evergreen shrubs and trees. Several studies have pointed out the importance of the genus in soil stabilization, and its ornamental, medicinal, and industrial applications. Ors or sarve kuhi (Juniperus seravschanica) has a wide distribution in Kerman province. However, in most areas, seedlings or young plants have very little distribution, which indicates the difficulty of seed germination due to various reasons. Also, rooting of its microshoots in micro culture techniques is considered to be difficult due to poor development and low frequency of rooting. Therefore, to overcome these problems, it needs to optimize an in vitro shoot multiplication and root induction and growth protocol. The purpose of this study was in vitro culture of apical shoots to induce their multiplication and rooting. Further, seeds were planted on micro culture media to study seed germination, callus induction and shoot and root regeneration from calli. The studied species was a population of J. seravschanica grown in Glochar (Raber, Kerman province).

Young shoots were collected from Glochar reserve of Rabor city (Kerman province) and kept at 4°C temperature for 3-7 days. The apical shoots with a size of 1.5 to 1.5 cm were separated and after sterilization, were cultured in woody plant medium (WPM) and Morashig and Skoog (MS) culture media without hormones to establish the samples. For shoot multiplication and growth, all samples were transferred to the medium containing plant growth regulators with optimized levels. Young shoots were transferred to rooting media (WPM, MS and ½MS) with different levels of IBA hormones. Seeds were separated from mature female cones, and full seeds (there are many empty seeds in cones) were exposed to cold treatment for 1-2 weeks. Then, they were sterilized and cultivated in MS (containing hormone) medium to study seed germination and callus formation from embryos. Callus regeneration and shoot formation and their rooting were investigated under appropriate hormone concentrations.

In the WPM medium, the highest percentage of shoot proliferation (40%) was obtained in the combined treatment of 3 mg/L BAP and 2 mg/L NAA. The shoot proliferation percentage was higher in MS media than WPM with 57% in concentrations of 0.2 and 3 mg/L of BAP and NAA, respectively. In both media, Proliferated shoots did not form any root in both media until four months after planting. The highest percentage of seed germination in MS medium was 33% at the concentrations of 5 mg/L BAP and 0.2 mg/L NAA. The highest rate of callus formation (40%) from embryos (seeds), as explants, was recorded in MS culture medium at the concentration of 0.2 and 3 mg/L of BAP and NAA, respectively. Calli produced shoots in in 3 mg/L BAP and 2 mg/L NAA in WPM medium. Root formation was not observed until six months, but about 10% of these regenerated shoots produced roots eight months after shoot proliferation.

It seems that various factors such as genotype, polyploidy or hybrid formation, slow plant growth (which is common in coniferal), or secondary compounds are the reasons for the low rate of regeneration, especially rooting. Also, the duration of cultivation can be effective in rooting.

Olive (Olea europaea) is a tree species whose seedling grows slowly and the propagation by seed is time consuming and increases the probability of genetic alterations. The most appropriate method to propagate olive is the use of shoot cutting as asexual propagation; however, semi-hardwood olive cutting is hard-rooting. The purpose of the current research was to facilitate rooting on shoot cutting of olive cv. Mari using the concentrations of 2, 3, and 4 g l−1 of indolebutyric acid (IBA) and naphthaleneacetic acid (NAA), singularly and in combination with each other. Used cultivation bed was sand. The research was done as a factorial based on a randomized complete block design with three replications. Rooting percentage, root number, root length, and root fresh and dry weights were measured. Based on the results of this experiment the highest percentage of rooting (83.30%), maximum root number (17.09), longest root (22.67 cm), and dry (2.30 g) weight were obtained in cuttings treated with 3 g l−1 NAA together with 3 g l−1 IBA. The highest fresh weight (4.83 g) was obtained in cuttings treated with 4 g l−1 NAA together with 3 g l−1 IBA. The lowest percentage of rooting, maximum root number, shortest root, and lowest fresh and dry weights were obtained in control.

Juniperus is the second most prevalent group of conifers on Earth. The reduction in viable seeds and natural regeneration is an important problem of Juniperus. Juniperus seravschanica is important in soil protection and is very resistant to frost and drought too. In this study, seed number in cone, seed emptiness and germination were studied on filter paper, peatmoss+ perlite mixture and in field in Galuchar, sarbijan and Dalfard (Kerman province) in populations. In addition, shoot proliferation and rooting in pot and in vitro culture was assayed. The average number of cone seeds in Glochar, Serbijan and Delfard was 4.86, 3.69 and 3.21, respectively. The average number of full seeds per cone was 1.1, 0.22 and 0.1 (22, 5.96 and 3.1%, respectively) in Glochar, Sarbijan and Dalfard, showing 78, 94 and 97% emptiness, respectively. In all media, seed germination percentage was very low (2-5%). No germination was observed in seeds without cold treatment as well as without scarification (making a hole in seed coat). 20 cm young shoots, after cold treatment and rapid immersion in IBA, grew as normal until 4 week and finally dried after 5-6 weeks. The young shoots (1-1.5 cm) cultured in MS and WPM media proliferated and regenerated new branches in WPM (40% regeneration) and MS (57%) media in combination of BAP and NAA (2+3 and 0.2+3 mgl-1, respectively for WPM and MS). No rooting was observed using IBA after 24 weeks.

Haworthiopsis limifolia, a cactus native to South Africa, is one of the herbaceous ornamental plants belonging to the Asphodelaceae family. This succulent perennial plant is known for its medicinal properties, including antibacterial and antifungal properties. The production and cultivation of this plant are traditionally associated with low yield and time consumption, which do not meet any needs of the market, and the producer faces problems with mass production. To solve these problems, new propagation methods, such as tissue culture, are used. The objectives of this study were to introduce a novel protocol for disinfecting Hawthorn for in vitro tissue culture and investigate the effects of different levels of growth regulators, including BAP (benzyl amino purine) and IBA (indole butyric acid), on the micropropagation of this plant. The results showed that 1.5% sodium hypochlorite solution for 10 minutes was the best treatment for the disinfection of the isolated cultures. In addition, our results indicated that the highest number of succulents (10) belonged to the treatment of 1.5 mg /L BAP. According to the mean comparison, the best treatment for rooting was 1.5 mg/L of IBA.  The 1.5 mg/l concentration of BAP led to the maximum number of succulents. Moreover, BAP had a more significant effect than IBA on cacti's rooting, shooting, and suckering.

This study aimed to access the rapid and improved propagation protocol production of Zamifolia plant using tissue culture.

For callus formation and regeneration, two independent factorial experiments were carried out in a completely randomized design with 3 replications in the tissue culture laboratory of the National Research Institute of Flowers and Ornamental Plants in 2018. In the first experiment, to induce callogenesis, 2,4-D (0, 1, 2, and 4 mg/l) and BA (0, 1 and 2 mg/l) were used. In the second experiment, or regeneration of explants, NAA (0 and 0.5 mg/l) and BA (0, 0.5, and 1 mg/l) were applied.

The results of the callogenesis experiment showed that a modified basal MS medium with concentration of 2 mg/l BA and 1 mg /l 2,4-D produced the best callus with 85% regeneration. At the regeneration stage of the callus, 0.5 mg/l BA and 0.5 mg NAA produced the highest number of seedlings and regenerated tubers (90%). Subsequently, single tuber seedlings were successfully adapted to 80%-90% in the cocopeat and perlite substrates.

The results were successful and could be possible to achieve the callogenesis and regeneration of Zamifolia plant with the formula presented and recommend as practical a protocol for commercial micropropagation of this valuable ornamental plant.

This studying have used to determine suitable concentration NAA , IBA and SA for destroying root half hard of henna . This experiment performed in one planning completely accidental in for repetition and 15 percent treatment . In this research growth regulator have applied for IAA in a level 2000 mg/lit, NAA regulator in three level 2000, 3000, 4000 mg/lit and SA in two level 200 , 400 mg/lit. Due to achived resolutions show that harmonica treatment have been caused to increase percent of cuttings and statistically difference between treatments Growth regulator showed meaning discrepancy as to example of level 5%. Although the highest percent destroying root cuttings was related to treatment 2000 mg/lit IBA and 3000 mg/lit NAA ,but the highest some of stem in treatment was 4000 mg/lit NAA+200 mg/lit SA and 2000 mg/lit IBA+200 SA and discrepancy of average these treatments didn’t observe with meaning difference in level 5%. Resolutions of this experiment showed that applied treatments have been caused to increase some of root in each cutting . Treatment 4000 mg/lit+200 mg/lit SA have been made the highest weigh of root and stem and weigh of drying root and stem

Tea plant ( Camellia Sinesis L. ) is a shrub or evergreen tree with a long life of 80 to 100 years old Theaceae family, with a height of up to 10 meters in wild conditions, but does not exceed 2 meters in the samples grown. Because it brushes the tea leaves every year to harvest it, they hold the plant up to 80 cm. The propagation of tea in the cutting method is the most common tea-growing method for producing moth-like plants. The greatest effect on rooting of cuttings was reported by auxins. Therefore, this experiment was based on a factorial arrangement based on a completely randomized design with two indole butyric acid (IBA) and naphthalene acetic acid (NAA) at four levels (0, 1000, 2000 and 3000 mg / l) and their interactions With three replications. At the end of experiment, rooting percentage, root number, root length and root dry matter percentage were measured. The results showed that rooting percentage of, IBA 2000 + NAA 2000 in root number and root dry matter percentage, IBA 3000 and root length, IBA 3000 + NAA 2000 treatment showed the best results.