جستجوی مقالات مرتبط با کلیدواژه « بروکلی » در نشریات گروه « زیست شناسی »

This research was conducted in order to investigate the application of melatonin and potassium sulfate on the quantitative and qualitative characteristics of broccoli (Brassica oleracea var. italica) under water stress. A factorial experiment was performed in a completely randomized design with three replications. The experimental factors included irrigation conditions at two levels (50 and 100% FC), melatonin at three levels (0, 150 and 300 µM) and potassium sulfate at three levels (0, 2 and 5 mM). The results showed that in the treatment of 300 µM melatonin and control irrigation (100% FC), the leaf area was twofold greater than in the treatment of 50% FC without the use of melatonin. The highest activity of the catalase enzyme was observed in the interaction of 50% irrigation level and 150 µM melatonin and the highest amount of malondialdehyde was observed in the same field capacity without the use of melatonin. The highest amounts of chlorophyll a and b were obtained in the interaction of 100% FC, 150 μM melatonin and 5 mM potassium sulfate. The highest amount of head yield was achieved in the interaction of 100% FC with the use of 300 µM melatonin and 5 mM potassium sulfate, and in this treatment, the highest nitrate reductase enzyme activity and the lowest nitrate accumulation in the crop head were observed. The highest content of proline and activity of the peroxidase enzyme were observed under conditions of 50% FC without the use of melatonin and potassium sulfate. The best treatment in most of the interaction traits was melatonin 300 µM, potassium sulfate 5 mM under control irrigation conditions (100% FC). Therefore, the use of these compounds is recommended to improve the physiological characteristics, yield and quality of broccoli.

Broccoli has been well known as a plant of high nutritional value. Application of broccoli peroxidase in different areas of clinical biochemistry, biotechnology and food industry enhances the interest for further study on the enzyme. The objective of this study was to evaluate the kinetic parameters of broccoli's peroxidase and their variations in the presence of methanolic extract of some endemic plants from Mazandaran. In this investigation, the crude extract from broccoli was homogenized, centrifuged, precipitated by ammonium sulfate and dialyzed. The enzyme activity was monitored in 414 nm with H2O2 and ABTS in phosphate-citrate buffer. The specific activity of the peroxidase was determined 0.58 U/mg in crude extract and 3.61 U/mg in dialysate. The optimum pH and temperature of the enzyme were obtained 4 and 30°C respectively. The extract of Artemisia tschernieviana, Eryngium caucasicum, Rosmarinus officinalis, Sambucus ebulus, Ligularia persica, Crataegus douglasii, Papaver rhoeas, Viscum album and Allium ursinum were prepared by maceration method. The enzyme activity was measured in presence of the extracts in different concentrations. The kinetic results shown that the extract of Crataegus douglasii, Rosmarinus officinalis, Papaver rhoeas and Artemisia tschernieviana in 33 µg/ml concentration inhibited the enzyme as 88.2, 79.5, 78.6 and 76.3% respectively. The extract of Ligularia persica shown no effect on maximum velocity of reaction while reduced the Km value by 4 times. Allium ursinu showed a partial inhibition on the peroxidase. Therefore, study on these plants for achieving to effectors on broccoli’s peroxidase, with pharmaceutical, agricultural and industrial applications could be recommended.