جستجوی مقالات مرتبط با کلیدواژه "حداقل غلظت مهار کنندگی" در نشریات گروه "زیست شناسی"

Thymus vulgaris and silver Nanoparticles has been demonstrated. Each of these agents for maximum impact requires a high concentration of substances affecting microorganisms. Assuming an anti-microbial agent with low concentration and in combination with other anti-microbial agents as the initial impact or more, A study to evaluate the effect of ethanol extract of propolis interference, Thymus vulgaris essential oil and silver nanoparticles on gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumoniae, Listeria monocytogenes) and Gram-negative bacteria (Proteus mirabilis, Klebsiella pneumonia and Pseudomonas aeruginosa) and on Candida albicans was performed. Microbial used in this study are taken from Iranian Research Organization for Science and Technology. Propolis is collected from different area of west Azerbaijan beehives. The ethanolic extract of propolis have prepared after 3 days. The interfering effects of these agents are evaluated by Micro-broth dilution method on Muller Hinton Broth in the presence of gentamicin as positive control for bacteria and Nistatin against Candida albicans. Acquired Minimum Inhibitory Concentration (MIC) show the effective interfering of these agents. The results of the Minimum Inhibitory Concentration (MIC) of propolis extract with silver nano particle on Klebsiella pneumonia was 25 µgr/ml, on Streptococcus pneumonia, Pseudomonas aeruginosa and Candida albicans was 50µgr/ml and on Staphylococcus aureus, Listeria monocytogenes and Proteus mirabilis was 100 micrograms per milliliter. MIC of Thymus essential oil with silver nanoparticles on Klebsiella pneumonia and Pseudomonas aeruginosa was 50µgr/ml, on Staphylococcus aureus, Streptococcus pneumonia and Candida albicans was 100 µgr/ml and on Listeria monocytogenes and proteus mirabilis was 200 micrograms per milliliter.

Antibiotic resistance has contrived use of medicinal plants with less side effects instead of common drugs. The present study was done to investigate antibacterial effects of garlic ethanolic extract against antibiotic resistant Staphylococcus aureus strains isolated from nasopharyngeal samples. Extraction was done using vacuum distillation (rotary). A total of 17 Staphylococcal strains were isolated from nasopharyngeal swabs of in- and outpatients in Ali-Ebne-Abitaleb hospital (zabol). Antibiotic resistance was determined using Kirby Bauer method and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of garlic extract was determined using micro-broth dilution method. Antibiotic resistance of the isolates was as follow: Penicillin (88. 2%)، Erythromycin (82. 4%)، Cefoxitin (82. 4%)، Trimethoprim (70. 6%)، Ampicillin (70. 0%)، Ceftazidime (29. 4%)، Tetracycline (23. 4%)، Ceftriaxone (23. 5%)، and Amikacin (5. 9%). The MIC and MBC for garlic extract were 1. 25 and 5 mg/ml resoectively. Garlic extract has high anti staphylococcal effects compared with common antibiotics and could be used as a benefit medicinal plant. However، it is suggest more studies to ensure its medical effects before wide medical usage.