جستجوی مقالات مرتبط با کلیدواژه "هیوسیامین-6- بتا هیدروکسیلاز" در نشریات گروه "زیست شناسی"

Hyoscyamus reticulatus L. is a main source of tropane alkaloids, hyoscyamine and scopolamine. In the present study, hairy roots were prepared from two-weekly cultures of cotyledon transformed by A7 strain of Agrobacterium rhizogenes. The effect of different concentrations of Fusarium graminearum extract (0, 5, 10 and 20 mg/L) at different exposure times (24, 48 and 72 hours) on growth rate, antioxidant enzymes activity, total phenol, tropane alkaloids contents and expression level of Hyoscyanamine-6-beta-hydroxylase (h6h) gene were investigated. Data analysis results showed that fresh and dry weight under influence of high concentrations of Fusarium fungus extract was reduced. The activity of antioxidant enzymes including catalase, guaiacol peroxidase and ascorbate peroxidase as well as total phenol content in treated hairy roots was enhanced compared to control. GC analysis showed that the highest levels of hyoscyamine and scopolamine (27.5 and 40.41% respectively) were found in 5 and 10 mg of elicitor at 48 h exposure time. RT-PCR expression analysis results revealed that the highest expression of this gene was obtained in Fusarium fungus extract (10 mg/l) at 48 h exposure time. Based on these results, it is concluded that the Fusarium graminearum elicitor can be used as an effective stimulant for the production of secondary metabolites, including tropane alkaloids.