جستجوی مقالات مرتبط با کلیدواژه « وینکریستین » در نشریات گروه « زیست شناسی »

Despite advances in diagnosis and therapy, cancer is still the main cause of death in world. Vincristine is one the major drug in tumor treatment. However, the anti-lymphoma effects of Vincristine on micro-RNA expression are not clear, especially in B cell lymphomas. The aim of the present study was to evaluate the effects of Vincristine on miRNA-15a3p and miRNA-15a5p genes expression Epstein‐Barr‐Virus (EBV)-infected transformed B cell lymphoma. In this study, the effects of cyclophosphamide on CO 88BV59-1 LCL – an EBV infected transformed B cell was evaluated. The cells were treated with Vincristine (0.05-50 µM), for 24-72 hours. MTT, flow cytometry and real-time PCR techniques were used for cell viability, and cytotoxicity evaluations and miRNA-15a genes expression, respectively. Vincristine significantly inhibited proliferation and induced cell death in EBV infected cell-line in a dose and time-dependent manner (P<0.05). There were no significant changes in the expression of miRNA-15a3p and miRNA-15a5p in treated cells compared to untreated cells (P>0.05). The results showed that cytotoxic effects Vincristine on CO 88BV59-1 LCL is independent on miRNA-15a3p and miRNA-15a5p expressions.

Vincristine is the most effective anticancer drug, widely used in the treatment of various cancers. Chromatin is composed of nucleosome units consisted of DNA and histone proteins. In the present study, for the first time the interaction of anticancer drug vincristine with histone H1 and core histone proteins in solution was investigated using fluorescence, UV and CD spectroscopy techniques. The results showed that in the presence of vincristine, fluorescence emission intensities were reduced in a dose dependent manner, representing quenching of the drug with aromatic residues located in the globular head domain of histone proteins. Stern-Volmer constant and binding affinity of the drug to histone H1 was higher than core histone proteins. The binding of vincristine to histones induced structural changes in circular dichroism spectra revealing increase of α-helix content of the histones. Moreover, vincristine increased UV absorbance of H1 and core histones at 210 nm (hyperchromicity). In conclusion it is suggested that vincristine, by its domains, can penetrate into globular head domain of histones. Also the binding affinity of vincristine to histone H1 is higher than to core histones, possibly because H1 is located in linker region which is more exposed and accessible to environment

Periwinkle (Catharanthus roseus (L.) G. Don)، a medicinal plant، which economiclly is highly valued because of its anti-cancer lalkaloids of vincristine and vinblastine. Since the availability of nitrogen (N) is expected to play an important role in the biosynthesis and accumulation of alkaloids in plants، the effect of N fertilization on the content of alkaloids of periwinkle was studied. the four levels of 0، 100، 150، 200 kg/ha N fertilization in a bulok completely randomized design with three replications. N fertilization significantly increased the content of alkaloids in leaves. This study showed that by increasing N fertilization dry weight increased. The highest level of alkaloid vincristine was in the 0 kg/ha of N fertilization and the highest level alkaloid vinblastine was in the 200 kg/ha of N fertilization.