جستجوی مقالات مرتبط با کلیدواژه « ascorbate peroxidase enzyme » در نشریات گروه « زیست شناسی »

A factorial experiment (based on completely randomized design) with three replications was conducted in greenhouse to investigate the effect of soil Zn deficiency on the genes expression of antioxidant enzymes catalase (CAT), ascorbate peroxidase (APX) and polyphenol oxidase (PPO) in Zn-efficient and -inefficient bread wheat cultivars. Bayat and Niknejad (Zn-efficient) and Hirmand and Karaj 1 (Zn-inefficient) cultivars were grown under soil Zn deficiency and normal conditions. The expression levels of three above-mentioned genes were measured using Real time PCR technique in leaf and root tissues of the cultivars at two growth stages: one month after germination (vegetative) and 30% of heading (reproductive). The results of variance analysis and mean comparison of treatments showed that the highest rate of CAT expression (5.74 fold) observed in Zn-efficient cultivars (Bayat and Niknejad) at reproductive stage under soil Zn deficiency conditions. Moreover, the expression levels of CAT and APX in leaves of Zn-efficient cultivars were more than those of inefficient cultivars under soil Zn deficiency. The highest expression level of PPO (5.62 fold) was obtained in the leaves of Bayat (Zn-efficient) cultivar under Zn deficiency conditions. Therefore, the results of the current study proposed the possible roles of the genes encoding three studied antioxidant enzymes in tolerance of Zn-efficient bread wheat cultivars against soil Zn deficiency.

In order to investigate the postharvest effect of sodium nitroprusside on vase life of cut rose, Utopia and Dolce Vita cultivars, an experiment was conducted as factorial in a completely randomized design with three factors and six replications. The first factor was included two cultivars of cut flowers of rose.  The second factor was four concentrations of sodium nitroprusside 0 (control) 20, 40 and 60 μM, which was applied by pulse treatment. The third factor was the time of application at 24 and 48 hours. The results of this research showed that sodium nitroprusside treatment had a significant effect on vase life, catalase activity, ion leakage and flower diameter at 1% probability level and for fresh and dry weight of flower and stem, Ascorbate peroxidase activity and water absorption rate had a significant effect at 5% probability level. The highest vase life of Utopia cultivar was in 40 μM sodium nitroprusside in 48 hours and in Dolce Vita, it was related to sodium nitroprusside concentration of 60 μM in 48 hours. The most antioxidant enzymes activity such as catalase and ascorbate peroxidase were obtained at concentrations of 40 and 60 μM sodium nitroprusside. In general, the results of this study showed that in Utopia using 40 μM sodium nitroprusside for 48 hours and in Dolce Vita 60 μM sodium nitroprusside for 48 hours were the best treatments that could improve the traits studied.