جستجوی مقالات مرتبط با کلیدواژه "bovine serum albumin" در نشریات گروه "زیست شناسی"

Methyl-Tert-Butyl Ether (MTBE) as a gasoline modifier is frequently added to fuels and used in plenty of worldwide applications. MTBE biodegradation in groundwater occurs slowly and produces water miscibility; therefore, it causes diverse environmental and human health concerns. The interaction of MTBE with bovine serum albumin (BSA) as a model protein at physiological conditions is investigated to illustrate the possible interactions of MTBE with the body’s proteins. Uv–visible, fluorescence, circular dichroism (CD) spectroscopy methods, and molecular modeling were used to analyze the MTBE’s effect on BSA structure and dynamics. The constant protein concentration and various MTBE contents were used for possible interactions. The protein structural analysis shows that MTBE binds to BSA via positive enthalpy and entropy via hydrophobic interactions. Molecular docking shows the participation of several amino acids in the MTBE-BSA interaction. The CD spectroscopy results show that the BSA structure was not changed in the MTBE concentrations utilized in the study. Molecular dynamics (MD) simulation results suggest that MTBE can slightly change protein structure in the last 50ns. Comparing experimental and MD simulation results demonstrated that the BSA secondary structure was maintained in the low concentration of the MTBE. The entropy and enthalpy parameters asserted the hydrophobic interaction was the major force in the interaction between the BSA and MTBE.

Recently, applications of nanoparticles in many fields of medicine have developed, due to their specific physical and chemical properties. Therefore, assessment of their toxicity especially in in vivo conditions is necessary.. The aim of this study was to compare the toxicity of Fe3O4 nanoparticles coated with biocompatible compounds and noncoated nanoparticles.. Wetted chemical method was used in order to synthesize Fe3O4 nanoparticles. The synthesized nanoparticles were coated with BSA (Bovine Serum Albumin) and DMSA (Dimercaptosuccinic Acid) and the coating interactions were investigated by FTIR. Magnetic and structure properties of Fe3O4 and coated Fe3O4 nanoparticles were evaluated by AGFM (Alternating Gradient Force Magnetometer), TEM (Transmission Electron Microscope) and XRD (X Ray Diffraction). Toxicity assessment of Fe3O4 and coated Fe3O4 nanoparticles were studied in mice by intra peritoneal injections during a one-month period. Liver enzymes (SGPT, SGOT, ALP, and LDH) were measured 7, 15 and 30 days post injection.. The synthesized nanoparticles have a single phase and spinel structure; their size distribution in the net form is around 5 to 11 nm and in the coated form is 17 to 25 nm. Some mice liver enzymes were changed due to the injection of both uncoated and coated nanoparticles (especially in groups which received concentrations of more than 100 mg per kg of mice weight). Liver enzyme changes were more considerable in groups that received DMSA or DMSA coated nanoparticles in comparison with those that received BSA or BSA coated nanoparticles. Chemical toxicity studies showed that there was no irreversible effect with concentrations less than 200 mg/kg for all control and treated groups.. The results indicated that liver enzymes were changed during seven and 15 days post injection, especially with high doses (200 mg/kg). The results of measurements 30 days post injection showed less change in comparison with the control and this indicates that there was no irreversible effect on the liver. Moreover, DMSA coated nanoparticles were more toxic in comparison with BSA coated nanoparticles..