جستجوی مقالات مرتبط با کلیدواژه "convolvulus arvensis" در نشریات گروه "زیست شناسی"

An experiment was condacted To study the allelopathic effect of saffron genotyps on bindweed in the department of agronomy and plant breeding of Isfahan University of Technology, Iran in 2012-2013. Experiment was arrange as a factorial in a completely randomized desiggn with three replication. Factors included 20 saffron genotypes obtained from different regions in Iran, ( Hamedan, Nishapur1, Nishapur2, Nishapur3, Nishapur4, Sabzevar, Mashhad1, Mashhad2, Natanz, Khaf, Khorasan, Torbatejam, Kashan, Gonabad, Torbat heydaryeh, Birjand, Yazd, Shiraz, Isfahan, Kashmar) and Aquous extracts were prepared from aerial parts of these saffron genotypes in concentrations of 0, 3, 6, 12.5, 25, 50 and 100%. Seedling traits of the attributes of Bindweed (as a target species) including radicle and plumule lengthes, radicale and plumule fresh weights, plumule dry weight, germination percent and velocity were measured.. Results showed that the effect of genotype and extract concentration and their interaction on measured traits were significant. Saffron genotypes had a different effect on the triats of bindweed. Genotypes "Khaf and Nishapur3" were proven to be the most allelopathic genotype, and genotypes "Kashan, Torbate jam and Gonabad" were found to leave minimal allelopatic effects on the target species. With increasing in aquous extracts concentration traits of target specie decreased, but the effect was diffreent. Maximum inhibition was observed in aquous extract concentrations of 100% and minimum inhibition was observed at aquous extract concentrations of 3% .

Field bindweed is a plant with scientific name Convolvulus arvensis L. This plant is a perennial plant of Convolvulaceae families. Developmental stages of generative meristem, pollen and microsporangium (early stages of pollen development) was studied using optical microscopy. The young flowers and buds were removed in different developmental stage, fixed in FAA70 and kept in 70% ethanol. Samples were embedded in paraffin after dehydration and sliced using microtome. Staining was carried out with Hematoxylin-Eosin. Prepared slides from different developmental stages were studied using light microscope and photographed. The results showed that anther is tetrasporangiated. Development of anther wall follows the dicotyledonous type, which is composed of an epidermal layerv, an endothecium layer, one middle layer and tapetum. The tapetum is secretory type at the beginning and plasmodial type at the end of anther development .Microspore tetrads is tetragonal and until the final stages of anther development is visible. Microspores were irregular in their early stages of development, but mature pollen grains are regularly. Pollen grains in polar view are spherical and equatorial view is ellipsoid, they are tricolpate and have three pores and bi- cellular at the mature form.

Nep1 is a natural bio-herbicide protein which has an effective necrosis stimulant in dicotyledonous weeds. This protein was first purified in 1995 form fermentation broth of Fusarium oxysporum. In this study, the nep1gene was isolated form F. oxysporum, after removal of signal peptide from the beginning of the gene; final pET16b-nep1 construct was cloned and transformed into E. coli BL21 (DE3). The recombinant Nep1 was produced inE. coli. Recombinant protein was purified, diluted and prepared for biological assay on weed. Two ml of recombinant Nep1 with 60 µg/ ml final concentrations was sprayed on Convolvulus arvensis. Our results showed necrosis and chlorosis symptoms were started on the plant leaves after 2 days, also significant necrosis on the leaves of C. arvensis was seen after 5 days. No necrosis lesion was seen in the negative control plants that sprayed only with buffer and denatured recombinant Nep1 in 100 ºC for 15 min. Discussion and Our results introduced recombinant Nep1 as a biological potent agent for biocontrol of C. arvensis.