Study on Development Stages of Generative Meristem, Flower and Male Gametophyte in Convolvulus arvensis L.

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Abstract:
Field bindweed is a plant with scientific name Convolvulus arvensis L. This plant is a perennial plant of Convolvulaceae families. Developmental stages of generative meristem, pollen and microsporangium (early stages of pollen development) was studied using optical microscopy. The young flowers and buds were removed in different developmental stage, fixed in FAA70 and kept in 70% ethanol. Samples were embedded in paraffin after dehydration and sliced using microtome. Staining was carried out with Hematoxylin-Eosin. Prepared slides from different developmental stages were studied using light microscope and photographed. The results showed that anther is tetrasporangiated. Development of anther wall follows the dicotyledonous type, which is composed of an epidermal layerv, an endothecium layer, one middle layer and tapetum. The tapetum is secretory type at the beginning and plasmodial type at the end of anther development .Microspore tetrads is tetragonal and until the final stages of anther development is visible. Microspores were irregular in their early stages of development, but mature pollen grains are regularly. Pollen grains in polar view are spherical and equatorial view is ellipsoid, they are tricolpate and have three pores and bi- cellular at the mature form.
Language:
Persian
Published:
Journal of Plant Research, Volume:29 Issue: 3, 2016
Pages:
484 to 493
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