جستجوی مقالات مرتبط با کلیدواژه « glutamine » در نشریات گروه « زیست شناسی »

Despite the importance of resistance protocol and glutamine on hypertrophy, their effect on myogenic genes expression process is still unknown. Therefore, the aim of the present study was to investigate the effect of an intense resistance session with glutamine on Myogenin and Myosin creatine kinase gene expression in male Wistar rats.

30 8-week-old male rats with an approximate weight of 220±20 were prepared and divided into three groups, control, intense resistance training, and intense resistance training with glutamine, in a simple random manner. The training groups participated in a resistance session of climbing the ramp with 4 sets, 5 repetitions, 30 seconds of rest between repetitions and 2 minutes of rest between sets. Glutamine was once a day powder dissolved in 100 cc of distilled water at a dose of 5.5 grams per kilogram of body weight every day for 5 days. The Extensor Digitorum long muscle tissue was sent to the relevant laboratory to study the expression of Myogenin and Myosin creatine kinase genes. The relative fold change method was used to check gene expression data at a significance level of 5%.

The gene expression results showed that myogenin and myosin creatine kinase gene expression levels increased significantly as a result of a high-intensity resistance training session with glutamine compared to the control group, and this value was more pronounced in the resistance training group (p<0.05).

It seems that an intense resistance training session is more effective than glutamine on the increase of myogenic genes.

Gladiolus is cultivated as a high-value ornamental plant in the worldwide and it has the highest cultivation area and the highest level of underground organs imports in Iran. In this study, in order to investigate indirect regeneration of Amsterdam and Advance Red cultivars an experiment was conducted in a completely randomized design with three replications. For callogenesis, bud sprout (apical section), corm slices (middle section) and basal plate (bottom section) explants of corm with 1 cm2 dimensions were cultured in MS medium with different concentrations of Picloram, Dicamba, BAP , kinetin, NAA and 2,4,5-T. At the regeneration stage, different concentrations of TDZ and GA3 were combined with Proline and Glutamine amino acids. Based on the results, in the medium containing 1 or 2 mg L-1 of Dicamba, the bud sprout explant showed the highest callogenesis (77.77% and 75.91% in Amsterdam and Advance Red, respectively). The highest percentage of regeneration (81.11% and 75.57% in Amsterdam and Advance Red, respectively) and the highest number of shoots (23.69 and 20.19 in Amsterdam and Advance Red, respectively) were obtained in 1 mg L-1 TDZ + 0.5 mg L-1 GA3 + 200 mg L-1 Proline + 200 mg L-1 Glutamine. The highest percentage of rooting (84.41% and 82.50% in Amsterdam and Advance Red, respectively) were produced in MS medium supplemented with 2 mg L-1 IAA + 500 mg L-1 activated charcoal. Produced plantlets from indirect regeneration were successfully adapted in greenhouse condition.

Siberian sturgeon is a suitable species for using in aquaculture industry. The purpose of this study was to investigate the effect of glutamine on growth and blood and immunity indices of Siberian sturgeon fingerling and to determine the optimal amount of glutamine in rearing conditions. This study was performed on 126 fish with a mean weight of 45.71±7.56g in six treatments (control, 5, 10, 15, 20 and 30g glutamine/kg diet) and three replicates during 8 weeks. The results showed that there was no significant difference in growth factors (P>0.05). However, final weight, final length, the percentage of increasing body weight, feed conversion ratio, specific growth factor and daily growth rate were higher in fish fed the diet containing 30g glutamine/kg diet compared to other treatments. The highest obesity coefficient was observed in fish fed the diet containing 5g glutamine/kg diet. Also, there was no significant difference in red and white blood cell count, hemoglobin, hematocrit, MCV, MCH, MCHC, neutrophil and lysozyme (P>0.05), but lymphocytes, monocytes, eosinophils, IgM and total immunoglobulin were significantly different (P<0.05). Glutamine had no significant effect on most of the blood parameters of Siberian sturgeon, but an increase in immunity parameters such as lymphocytes, monocytes, total immunoglobulin and IgM was observed. It seems that the addition of glutamine to the basal diet is not necessary in this age and culture conditions and the basal diet supports the needs of fish.