جستجوی مقالات مرتبط با کلیدواژه « phenole » در نشریات گروه « زیست شناسی »

Teucrium polium, from Lamiaceae family, is a medicinal plant. Due to overexploitation, low distribution and habitat destruction, this plant, is facing extinction. Plant biotechnology and tissue culture is an alternative method for plant secondary metabolites production. The present study aimed to enhance the production of secondary metabolites and antioxidant capacity of Teucrium polium L. using methyl jasmonate and salicylic acid elicitors. Leaf explants from hydroponically cultured 4-month-old plants were inoculated on Murashige and Skoog medium (MS) containing 2, 4-dichlorofenoxy acetic acid (2, 4-D) in concentrations of 0, 0.5, 1, 1.5 mg/L alone or in combination with benzyl aminopurin (BAP) at, 0.5, 1, 1.5, 2 mg/L concentrations. According to the results, the best callus induction (100%) was achieved at MS medium containing 1.5 and 2 mg/L BAP. Calli obtained from this treatment were subsequently treated with Methyl Jasmonate or Salicylic Acid at 0, 50 and 100 μg/L concentrations. After 40 days, the content of phenolics, flavonoid compounds and total antioxidant capacity were evaluated in a completely randomized design with three replications. Based on biochemical assays, the greatest total phenol content, flavonoids and total antioxidant capacity belong to calli treated with 100 μg/L acid salicylic (SA) and after that to cali treated with 50 μg/L methyl jasmonate(MeJa), while the difference between them was significant. The results of means comparison showed applying 100 μg/L SA on calli in medium with 1.5 mg/L BAP had the greatest effect on total flavonoid content. Therefore, the use of appropriate concentrations of these elicitors can play an effective role in enhancing the medicinal compounds in Teucrium polium.

The aim of this study was to investigate the performance of antioxidant and antimicrobial activity of hydroelectric extract of Anethum graveolens. In this study, the well test and paper discs were conducted to investigate the anti-microbial activity, and to determine the total phenolic compounds by the Folin-Siouketto method and for DPPH radical scaling activity by measuring the capacity Radical scaling of 1-1-2 pecril of hydazine as well as total antioxidant activity (TAC) were performed by the mentioned method. The results of the antibactrial performances showed that the plant had no inhibitory effect on the growth of Aeromonas hydrophila at concentrations of 125, 250, 500 and 1000 micrograms per milliliter. Also, the amount of phenolic, DPPH and total antioxidant compounds in the plant were 49.85 ± 8.92, 62.51 ± 1.6 65.6 and 84.69 ± 3.65, respectively. generaly, the results of this study showed that the hydroalcoholic extract of the plant has a significant antioxidant effect, and even the antioxidant power of the hydroalcoholic extract in different methods has shown a different level in comparison with the similar work of other researche.

In order to study the effect of chemical fertilizer, manure and their combination on phytochemistry of Thymus daenensis L., this research was coducted using a randomized complete block design with split plot in time layout including 12 treatments and 3 replications. Treatments were 3 rates of N. P. K chemical fertilizers, 4 rates of manure fertilizer, combination of chemical fertilizer and manure in four rates and control (no fertilizer application). The essential oil was extracted by hydro distillation method by using a Clevenger-type apparatus and were analyzed by GC and GC-MS. The total phenols were measurred by spectrophotometry. The results were showed that the highest phenolic content (57.17 kg/ha), essential oil yeild (3.88%) and thymol (86.68%) were obtained by application of 40 kg N, 32 kg P and 32 kg K combined with 25 ton/ha manure , but the highest carvacrol percentage (10.11%) obtained by application of 25 ton/ha manure. In all treatments the thymol, carvacrol and e-caryophyllene were the main essential oil components and different treatments had no effect on essential oil composition. There is a negative significant correlation was observed between thymol with carvacrol and e-caryophyllene. This findings may provide a hopeful perspective to replacing chemical fertilizers by organic fertilizers. In conclusion, the quantitative and qualitative yield in combined applicaion of chemical and organic fertilizers may be superior than chemical or organic systems alone and it can be concluded that this combined system is adapted with sustainable agriculture.

Allelochemical compounds inhibit weed growth and have undesirable effects on crop plants that should attended in croprotation. Canola is a allelopathic plant that is cultivated in north of Iran .Soybean is a strategic plant that is planted after canola and be affected by released compounds of canola residue. In this research effect of different concentrations of aqueous extract of canola (cv Hyolla 401) includes 0(control), 5%, 10%, 15% and 20% on growth and some of the organic compounds such as soluble sugars, proline, protein, phenolic compounds in root and nodule of soybean (cv Gorgan 3) after 50 days were studied until their response to canola allelochemicals were evaluated. The results showed that by increasing of canola extract concentration fresh and dry weight soybean root did not change significantly while in nodule increased. Amounts of proline and total protein in soybean root in higher concentrations decreased and in nodule also decreased mostly. By increasing extract concentration of canola phenolic compounds in root increased but in nodule decreased that was significant.