جستجوی مقالات مرتبط با کلیدواژه « Raw264.7 » در نشریات گروه « زیست شناسی »

Previous data have shown that the thiazolidinediones and their derivatives are found to possess divergent and very interesting biological activities such as, antioxidant and antibacterial activities. In this study, we investigated the cytotoxicity and inhibitory effects of synthesized TZD-OCH2CH3 on lipopolysaccharide (LPS)-induced over-expression of inducible nitric oxide synthase (iNOS) using Raw 264.7 cells. To assess whether the tested TZD-OCH2CH3 affected cell viability, RAW 264.7 cells were incubated with LPS (1μg/ml) in the presence of different TZD-OCH2CH3 concentrations (0-300 μg/ml). The results of MTT assay showed that TZD-OCH2CH3 had cytotoxicity at higher concentrations of 50 μg/ml (IC50=120 μg/ml). We then further evaluated the inhibitory activity of different TZD OCH2CH3 concentrations (30 and 60 μg/ml) against NO production in LPS-stimulated RAW 264.7 cells. The synthesized TZD-OCH2CH3 was found to have the most significant inhibitory effect on NO production in LPS-induced RAW 264.7 cells. These findings demonstrated that TZD-OCH2CH3 has good anti-inflammatory activity and thus has great potential for use in the control of various inflammatory diseases.

Thiazolidinones are important biological compounds that specified have anticancer and anti-inflammatory properties. Recently a new derivative of thiazolidinones called 5(2,4-bis4-ethoxy phenyl azo-3-hydroxy benzylidene)-2,4 thiazolidinone (TZD-OCH2CH3) synthesized and its antioxidant properties has been proven. Since, anti-tumor compounds can induce apoptosis in cancer cells through inhibition of NF-kB, so in this study, the anti-inflammatory effects of this compound (TZD-OCH2CH3) and its ability to inhibit NF-kB on the cell lines Raw264.7 examined. In this study, at first the cells in medium containing FBS, DMEM and antibiotics were cultured and toxicity screening of the concerned compound TZD-OCH2CH3 study with concentrations (0-120µg/ml) was performed. MTT test was conducted to investigate the inhibitory effect of different doses of the compound after cells incubation at 37°C for 24 hours and absorption of the samples was read by ELISA reader in 490 nm wavelength. Then, to investigate the inhibitory effect of the compound on NF-kB the kit specified to measure NF-kB was used. According to tests of growth inhibition concentration, 50% (IC50) Raw264.7 cells treated with TZD-OCH2CH3, 115 μg/ml was obtained. On the other hand, Measuring inhibition of NF-kB on Raw264.7 with concentration of 30 and 60 µ g/ml thiazolidinone shows the inhibition of inflammatory factor this compound. According to studies, the results show that our concerned compound in cell order Raw264.7 with regard to inhibiting NF-kB with (IC50=48 g/ml) has significant anti-inflammatory effects and can considered be used as a promising chemotherapeutic agent in the treatment of cancer.