New derived Thiazolidinone effect on inflammatory pathways with emphasis to inhibit transcription factor NF-κB

Thiazolidinones are important biological compounds that specified have anticancer and anti-inflammatory properties. Recently a new derivative of thiazolidinones called 5(2,4-bis4-ethoxy phenyl azo-3-hydroxy benzylidene)-2,4 thiazolidinone (TZD-OCH2CH3) synthesized and its antioxidant properties has been proven. Since, anti-tumor compounds can induce apoptosis in cancer cells through inhibition of NF-kB, so in this study, the anti-inflammatory effects of this compound (TZD-OCH2CH3) and its ability to inhibit NF-kB on the cell lines Raw264.7 examined. In this study, at first the cells in medium containing FBS, DMEM and antibiotics were cultured and toxicity screening of the concerned compound TZD-OCH2CH3 study with concentrations (0-120µg/ml) was performed. MTT test was conducted to investigate the inhibitory effect of different doses of the compound after cells incubation at 37°C for 24 hours and absorption of the samples was read by ELISA reader in 490 nm wavelength. Then, to investigate the inhibitory effect of the compound on NF-kB the kit specified to measure NF-kB was used. According to tests of growth inhibition concentration, 50% (IC50) Raw264.7 cells treated with TZD-OCH2CH3, 115 μg/ml was obtained. On the other hand, Measuring inhibition of NF-kB on Raw264.7 with concentration of 30 and 60 µ g/ml thiazolidinone shows the inhibition of inflammatory factor this compound. According to studies, the results show that our concerned compound in cell order Raw264.7 with regard to inhibiting NF-kB with (IC50=48 g/ml) has significant anti-inflammatory effects and can considered be used as a promising chemotherapeutic agent in the treatment of cancer.