جستجوی مقالات مرتبط با کلیدواژه « فلزات سنگین » در نشریات گروه « باغبانی »

Environmental stress in plants, particularly due to heavy metal presence, poses a significant challenge to their growth. Manganese, an essential micronutrient, can become detrimental when accumulated in soil and plants. To assess the impact of silicon on linseed (Linum usitatissimum L.) under manganese stress, a factorial experiment was conducted. Linseed plants were grown in hydroponic culture with four different manganese levels (2, 250, 500 and 1000 μM) along with five silicon treatment levels (0, 0.5, 1, 1.5 and 2 mM). Results revealed that increasing manganese stress levels led to a rise in proline, soluble sugars content, and manganese in the root and shoot, accompanied by a decrease in other studied traits. Notably, the application of 2 mM silicon consistently yielded the highest values across all manganese levels for the examined traits. Silicon application enhanced proline and soluble sugars content in leaves, consequently increasing plant photosynthetic capacity and leading to greater root and shoot dry weights under various silicon levels. The use of silicon significantly ameliorated the harmful effects of manganese in linseed under manganese stress conditions. Therefore, 2 mM silicon is recommended as a beneficial solution in areas contaminated with manganese, which can lead to the reduction of this heavy metal in the root and shoot of plants.

The use of plant hormones has been introduced as a simple and suitable strategy to reduce the adverse effect of heavy metals in plants. To investigate the effect of salicylic acid (SA) in reducing lead (Pb) stress in tomato (Lycopersicon esculentum L.), a factorial experiment was conducted based on completely randomized design with three replications in the greenhouse of Razi University. Factors included soil Pb contamination at three levels (0, 150 and 300 mg kg-1 soil as Pb(NO3)2) and SA at three levels (0, 250 and 500 μM). Results showed that the highest proline content (23.4 mmol g-1) and Pb concentration (4.26 mg kg-1) were obtained in 300 mg kg-1 of Pb and 0 μM SA, which shows an increase of 303 and 387%, respectively, compared to the control (without Pb and SA). Also, the highest amount of soluble sugars (0.48 mg kg-1), plant height (43.5 cm), root length (18.3 cm/plant), shoot dry weight (7.48 g/plant), and leaf area (263.1 cm2/plant) were found in the treatment of 500 μM SA and 0 mg kg-1 Pb. In general, the results showed that the application of SA, as a plant hormone, is a simple and convenient method to reduce the adverse effects of Pb stress in tomato.

Accumulation of heavy metals in medicinal plants can have dangerous consequences for human health. This study investigates the concentration of heavy metals including zinc, lead, copper, and cadmium in the soil, aerial parts (shoot), and roots of the medicinal plants Phlomis olivieri Benth. and Stachys inflata Benth. around the Zeh-Abad lead and zinc mine in Qazvin province. The soil and plant samples were collected and prepared, and the total concentrations of heavy metals in the samples were measured by an atomic absorption spectroscopy in three replicates. The results showed that, unlike copper and cadmium, the concentrations of lead and zinc in the soil, shoot, and roots of the studied plants were several times greater than the standards (i.e., EPA and WHO). The average concentrations of zinc and lead in the contaminated soil were 1791 and 2855 mg/kg, respectively. The average concentrations of zinc and lead in the roots of Ph. olivieri were 394 and 56 mg/kg, respectively, and in the shoot of this plant were 442 and 76 mg/kg, in the roots of S. inflata, were 452 and 38 mg/kg, and in the shoot of this plant were 501 and 68 mg/kg, respectively. The highest concentration of zinc was measured in the root of S. inflata (i.e. 694 mg/kg) and the highest concentration of lead was determined in the shoot of Ph. olivieri (i.e. 140 mg/kg). The transfer factor values for the lead and zinc in both plants were higher than one; in the case of the copper and cadmium, its values were lower than one. Considering the harvesting of these two medicinal plants by the native people in the region, the results of this study can be a warning for the health of these people.

 Heavy metals are one of the most important environmental contaminants, particularly in soil and water sources. Mining and metal mining activities are major factors in soil contamination and generally surface soil around mines contain high amounts of these metals. Lead (Pb) is one of the heavy metals and important pollutant in arid ecosystem. The use of plants to remove contaminated soil or phytoremediation is an economical method. Today, due to increasing the pollution of soil sources and resultant problems, identification of the resistant plant species against soil pollution is essential. Using lead-contaminated soils requires their decontamination and improvement. There are different methods to remove these pollutions, one of them is the use of phytoremediation to remove pollutants from water and soil or to reduce them. Among the plants that as an ornamental plant can have a covering role in the green space and also perform the work of phytoremediation is the ornamental cabbage plants (Brassica oleracea L.). In this regard, this study was conducted with the aim of investigating the effect of lead heavy metals and the use of brown algae (Polycladia indica) and spirulina (Arthrospira platensis) as biofertilizers on ornamental cabbage plants in the direction of plant treatment. In addition, due to the presence of polysaccharide compounds such as carrageenan and alginate in the cell wall, algae (seaweeds) have a higher ability to absorb many heavy metals.

 The experiment was conducted as factorial based on a completely randomized design in 2020 at the research greenhouse of Zanjan University. The studied factors including lead from lead nitrate source Pb(No3)2 with three concentrations (0, 25 and 50 mg/kg) were applied to the potting soil in four replications in two stages with an interval of two weeks. The second factor was included no algae, brown algae (Polycladia indica) and spirulina (Arthrospira platensis), which occurred in four replications. The measured traits included morphological traits: number of leaves, leaf surface index. Wet and dry root weight, and physiological traits included total chlorophyll content, total leaf antioxidant, peroxidase enzyme, glycine betaine, malondialdehyde, and leaf lead and phosphorus content. Data analysis was performed using SAS software and means were compared by LSD method.

 The results of the variance analysis showed that different levels of lead and the application of algae had a significant effect on the number of leaves per plant, leaf area index, weight, and drying of roots, total chlorophyll, and antioxidants. Moreover, the interaction effect was significant for leaf area, root fresh and dry weight, antioxidants, and leaf lead content. The simple effect of lead at p≤0.01 significantly affected glycine betaine, malondialdehyde, and leaf phosphorus. When comparing the average mutual effects of lead and algae application, it was found that the treatment with no use of lead and spirulina algae resulted in the highest weight and dry weight of the root, with an average of 11.19 and 3.625 grams, respectively. Additionally, despite the decrease in dry weight of the root due to increased lead concentration, using algae, especially for ornamental cabbage (Brassica oleracea L.), increased the dry weight of the root. The presence of natural plant hormones, organic substances, carbohydrates, fiber and amino acids in algae accelerates rooting, reduces stress caused by heavy metals and absorbs more water due to the presence of o developed root system. Also according to the results of comparing the amount of leaf lead with increasing lead, algae as an auxiliary factor can reduce the amount of uptake in the plant. Leaf phosphorus was also significant due to the simple effect of lead and algae. The highest amount of phosphorus with an average of 0.56% was observed in spirulina and the lowest with 0.48% was observed in control.

 In general, due to the toxicity of lead metal even in low concentrations, sufficient attention should be paid to the sources of this pollutant entering the environment. In this study, the effective parameters on the uptake of heavy metal lead from the soil by spirulina and brown algae were investigated. The findings of this study indicate that the ornamental cabbage plant is capable of sustaining its growth in the presence of lead and has a high resistance to this heavy metal while simultaneously absorbing it from the soil. Furthermore, the addition of algae as an auxiliary factor can improve the growth of ornamental cabbage under adverse conditions. Therefore, it is recommended that this plant be further examined for its potential to absorb other heavy metals.

In order to investigate the effect of sodium nitroprusside levels on growth and quantitative and qualitative characteristics of safflower (Carthamus tinctorius L.) in arsenic contaminated soils, a pot experiment was conducted in a research greenhouse of Faculty of Agriculture, Islamic Azad University, Varamin-Pishva Branch, in 2016-2017 in factorial experiment and in the form of design Completely randomized in 3 replications. The experimental treatments included of sodium nitroprusside spraying at three levels: (0, 50 and 100 μm) and soil contamination with arsenic at four levels (0, 30, 60 and 90 mg of arsenic per kg of soil). The results showed that arsenic and sodium nitroprusside had a significant effect on grain yield, 1000 grain weight, catalase enzyme, malondialdehyde content, proline content and safflower arsenic content. The highest grain yield was obtained in sodium nitroprusside treatments in application of 100 μM (with an average of 1.01 g / plant), and the lowest was related to control treatment (no application) with mean of 0.83 g / plant. By increasing the concentration of arsenic, yield and yield components decreased, and the plant increased its proline content to cope with stress. Application of sodium nitroproside reduced stress in plants under arsenic toxicity. No significant differences were observed between the concentrations of sodium nitroprusside spraying. With increasing arsenic levels, the amount of arsenic content increased and sodium nitroprusside reduced the amount of arsenic in the seed. In general, use of sodium nitroprusside as a regulator in the metabolism of free oxygen radicals reduces the effects of oxidative stress caused by soil contamination with arsenic.

The lead-zinc industries are known to be one of the high-risk industries in terms of the environmental issues. In this research, 15 samples of waste (cake) from Zanjan Lead and Zinc Factory were collected and six samples were selected for adding to soil according to type and concentration of heavy metals. Then, lettuce was planted in soil samples treated with different cakes. After harvesting the plants, the plant growth indices, concentration of heavy metals in plant roots and leaves, and available concentration of heavy metals in the cultivated soil were measured. This research was carried out as a completely randomized design, in which, six types of cakes were tested at three levels (0.25, 0.5 and 1%) with three replicates and three control samples (without adding the cakes). Results indicated that the highest amount of pollution for total concentration of heavy metals was related to Cd (168333 mg/kg), Zn (100000 mg/kg), Fe (91666 mg/kg), Mn (25566 mg/kg) Pb (13483 mg/kg) and Ni (7966 mg/kg), and for available concentration of heavy metals was related to Mn (21400 mg/kg), Zn (16400 mg/kg), Cd (4520 mg/kg), Pb (2371 mg/kg), Ni (194 mg/kg) and Fe (11.2 mg/kg). Available concentration of heavy metals in soils treated with cakes was reduced by uptake of these metals by lettuce

Culturing, isolation, purification, and identification of cyanobacteria collected from wheat ield soil, in five stations around the industrial areas in Yazd province (Iran) were conducted in this study. dentification of taxa was based on morphology and molecular methods. Cluster analysis and principal component nalyses performed using SPSS software and rate of resemblance among the taxa were investigated. The hylogenetic analyses carried out according to 16S rRNA gene sequences data, and the phylogenetic relationships were ssessed by Maximum Parsimony, Maximum Likelihood and Bayesian Inference. The results of this study ndicated presence of 32 species included in four orders of cyanobacteria. Among these orders, Oscillatoriales with three families, eight genera and 13 species showed the highest species diversity, whereas Chroococcales with two families, hree genera and four species, exhibited the lowest diversity. Among isolated taxa, Oscillatoria ornata, O. ngelmanniana, Jaaginema pallidum, Pseudanabaena minima, Stenomitos frigidus, Nostoc edaphicum, and Gloeothece epidariorum, are reported as new records for algal flora in Iran. Distributions and descriptions of the new taxa were also resented.

Zataria multiflora Boiss. is one of the valuable and endangered medicinal plants used in food and medicine industries. A study was conducted in a completely randomized design with three replications in four habitats of Isfahan, Fars, Yazd and Hormozgan provinces to investigate some of the mineral elements of Zataria multiflora Boiss. (Shirazian thyme) and also for comparing the accumulation of heavy metals in some natural habitats of the country. The mineral elements of this medicinal plant including micronutrients and a number of essential and high-consumption elements such as iron, zinc, manganese, copper, magnesium, sodium, calcium, potassium and a number of metals such as chromium, cobalt, silicon, lead, arsenic, nickel, cadmium, vanadium, totally 17 elements, were measured by using an inductively coupled plasma measuring apparatus (ICP-OES). The results of analysis of variance showed a significant difference among habitats for all elements except arsenic, cadmium and mercury (P

Nowadays, phenolic compounds and plant growth regulators such as salicylic acid has been prescribed to reduce the adverse effects of different stresses. This greenhouse experiment was conducted in 2013, as a factorial randomized complete block design with 3 replications, to examine the effects of lead and salicylic acid on basil (Ocimum basilicum) plant, cv. Keshkeni Luvelou. Factors included 4 levels of lead [0 (control), 100, 200 and 300 mg/kg of soil] and foliar application of salicylic acid at 3 levels [0 (control), 50 and 100 ml/L]. Results showed that addition of lead significantly reduced leaf area, number of leaves, plant hight, fluorescence and chlorophyll content of the plants and increased proline, soluble carbohydrates and guaiacol and ascorbate peroxidase enzymes. In addition, salicilic acid spraying had significant (P≤0.01) influence on all traits. Spraying salysilic acid at concentration of 100 ml/L at the fourth level of lead (300 mg/kg soil) decreased guaiacol, ascorbate peroxidase and soluble carbohydrates by 78.6, 73.8 and 24.3%, respectively, compared to the control. In this study, the interaction between salicylic acid and lead on leaf area, plant height, leaf chlorophyll content, fluorescence, proline, soluble carbohydrates, guaiacol and ascorbate peroxidase was significant. In general, the results of this experiment suggested that salicilic acid plays a moderating role, reducing the negative effects of lead toxicity on the studied traits of basil.

In order to investigate the interaction of cadmium (Cd) and different levels of organic and inorganic nitrogen (N) on some physiological and morphological traits of purslane (Portulaca oleraceae) medicinal plant, an experiment was conducted in Research Greenhouse of Shahrekord University, in 2012. Nitrogen treatments were consisted of four separate application levels (60 and 120 mg N/kg soil in the form of urea and cattle manure), three levels of combined-fertilizer application (90 mg N/kg as ratios of 2:1, 1:1 and 1:2 from urea and cattle manure, respectively), and control (no fertilizer and manure) as the first factor and two levels of Cd (10 mg Cd per kg soil and no Cd application) as the second factor. Results showed that Cd significantly decreased leaf chlorophyll, leaf carotenoids, plant height, number of leaves, leaf dry weight, stem dry weight and shoot dry weight. However, increasing N application significantly increased the aforementioned traits under Cd and without Cd application. The greatest purslane yield was obtained in 120 mg N/ kg as cattle manure. On the other hand, there was no significant difference between 120 mg N/kg as urea and combined treatment (1:1) as cattle manure and chemical fertilizer. In general, it can be concluded that application of animal manure not only would increase potential yield of purslane, but also mitigates the Cd stress effect on growth of purslane crop.

To evaluate the effect of salinity on phytoremediation of contaminated soil by plant sunflower, an experiment in the greenhouse, College of Agriculture, Islamic Azad University of Varamin in 2013. Factorial experiment was conducted in a completely randomized design experiment consisted of first factor is soil contamination with cadmium at four levels: 0, 30, 60 and 90 mg of cadmium per kg of soil salinity and the second factor of four levels of NaCl: 0, 2, 4 and 6 dS m. The highest cadmium leaves and stems of treatment (90 mg of cadmium per kg of soil and 6 dS m) with an average of 6.33 mg per kg of dry matter and the lowest related to treatment (90 mg of cadmium per kg of soil and control) with 13.13 mg per kg and the highest cadmium absorption coefficient in saline (6 dS m 30 mg of cadmium per kg soil) with 0.199 and with the treatment (control 90 mg of cadmium per kg of soil 0.095), the highest and lowest percentage of protein and 90 milligrams of cadmium per kilogram of soil control respectively 32 and 23 percent, respectively. The highest percentage of protein in the treatment 6 (dS m) with an average of 38 percent was achieved. The protein content of the control (salinity), respectively. The highest amount of chlorophyll a to control the salinity of 6 dS m with an average of 1.096 and 0.767 mg per gram of fresh weight, respectively. The highest and lowest proline content in 90 mg of cadmium per kg of soil and control with an average of 621 and 523 micromoles per fresh weight, respectively .Hieghest and lowest relative water content in irrigation and 90 mg of cadmium per kg soil respectively by 76 and 60 percent, respectively. Most RWC control (salinity) with an average of 74 percent was achieved. Lowest levels in treatments 6 (dS m). The result showed that salinity and cadmium on relative water content, proline content, the content of cadmium in leaves and stems, the absorption coefficient of Cd, protein, chlorophyll a significant effect, but the effect EC on oil percent was not significant. Interaction between cadmium and salinity on cadmium content in leaves and stems, cadmium absorption coefficient, chlorophyll A was significant. Salinity increased plant availability and cadmium so that the cadmium content in wheat increased significantly with increasing salinity.

Presence of heavy metals (such as lead, Pb) in plants is one of the major environmental stresses that decrease plant growth. Therefore, it is essential to determine the threshold of the plant's tolerance to these elements. In this regard, to evaluate the effect of different concentrations of Pb on eco-physiological traits of petunia plant, an experiment was carried out in the hydroponics greenhouse of Tabriz University, Tabriz, Iran, as a completely randomized design with 8 treatments and 4 replications. Treatments consisted of different concentrations of Pb (0, 0. 25, 0. 5, 0. 75, 1, 1. 5, 2 and 4 mg/L as Pb(NO3) 2). After applying the treatments, at the end of the growing season, the eco-physiological indices including photosynthesis, transpiration, stomatal conductance, stomatal resistance and leaf temperature were measured. Results showed that concentrations higher than 0. 75 mg/L Pb (NO3) 2 reduced the rate of photosynthesis, transpiration and stomatal conductance as compared to control. The highest stomatal resistance (8. 754 M/m2. s) was observed in 4 mg/L treatment; though it didnt have significant difference with 1. 0, 1. 5 and 2 mg/L treatments. The lowest stomatal resistance (7. 285 M/m2. s) was seen in 0. 25 mg/L treatment, which had no significant difference with control. The highest leaf temperature (33. 26 ْC) was observed in 2 mg/L treatment; though it didnt have significant difference with 1. 5 and 4 mg/L treatments. The lowest leaf temperature (26. 19 ْC) was obtained in the control treatment. After 0. 75 mg/L, the increase in leaf temperature was sensible. Based on the results, it appears that in soils having Pb concentration of ≤ 0. 75 mg/L, petunia plants are recommendable for cultivation in landscapes, accumulation of Pb, and phytoremediation.

The current research has been carried out to evaluate the effect of zinc and cadmium on the yield of white radish plant in a calcareous soil using factorial experiments based on completely randomized design with different zinc (0¡ 20 and 50 mg/kg) and cadmium (0¡ 10 and 20 mg/kg) levels and three replications. The results showed that there is no significant difference on shoot dry and wet weight¡ plant height¡ length and number of leaves by pollution increment¡ unlike root of plant. Moreover¡ it has been found that the amount of zinc and cadmium uptake by plant is directly dependent on their concentration in root environment. However¡ the negative interaction of zinc and cadmium was observed in the studied soil and plant. In addition¡ the amount of transfer and accumulation of the aforementioned elements was not the same in different parts of the plant which represents a reduction in transfer coefficient of the metals from root to shoot as one of defensive mechanisms of plant against high densities of heavy metals in soil. Therefore¡ white radish plantation either in polluted soil or its irrigation with polluted water by zinc and cadmium is not recommended due to more accumulation of these metals in the edible parts of plants than other parts examined.

Mining، industrial and agricultural activities can result in considerable soil pollution by heavy metals (HMs). One of the methods to control this pollution is application of adsorbent minerals. The purpose of this study was to compare the effects of adsorbent minerals (bentonite، zeolite and sepiolite) on transfer of some heavy metals (i. e.، Pb، Zn and Cd) from soil to tissues of sunflower (Heliantus annuus L.). Treatments included: Non-polluted soil، HM-polluted soil، polluted soil + bentonite، polluted soil + zeolite، and polluted soil + sepiolite. Sunflower seeds were planted in different pots containing the above treated soils. After 70 days of growth، plants were harvested and HMs content was measured. Results revealed that the uptake of HMs from non-polluted soil was lower than that of other treatments. In the polluted soil + sepiolite treatment، the added sepiolite increased plant Cd and Zn uptake by 0. 37 and 7. 17 mg/kg، respectively. However، the differences in HMs uptake were not significant for other treatments. Zeolite addition decreased plant uptake of Zn and Cd by about 12 and 0. 21 mg/kg، respectively، while bentonite addition reduced Pb uptake by about 3. 05 mg/kg، without any significant difference for the other treatments. Zn uptake was higher by about 12. 2 mg/kg in plant shoots as compared to plant roots. On the contrary، the roots took up higher amounts of Cd and Pb، as compared to the shoots. Despite high concentration of the HMs in the polluted soils، considerable tendency of sunflower for uptake، and a high adsorption capacity of the applied minerals، the HMs uptake by sunflower and surface adsorption by the minerals were very low، probably due to HMs precipitation in the soil.

To study the activity of some antioxidant enzymes and accumulation of compatible osmolytes affected by different concentrations of chromium (Cr+3) (0، 5، 10، 15 and 20 mg/kg soil) combined with different levels of manure (0، 10 and 20 ton/ha) in basil (O. basilicum L.)، a factorial experiment، based on completely randomized design with four replications، was carried out at Research Greenhouse of Zabol University in 2012. Results of this study indicated that increasing the level of Cr+3 from 0 to 20 mg/kg soil، when 20 ton/ha manure was applied، decreased the activity of catalase (CAT)، ascorbate peroxidase (APX) and glutathione peroxidase (GPX) enzymes decreased from 0. 008، 0. 537 and 0. 081 to 0. 006، 0. 305 and 0. 071 µmol H2O2/mg protein، respectively. This may be caused by the improvement in physiological activities of the plant in the presence of organic compounds. It seems that application of different amounts of manure led to an increase in leaf proline and carbohydrates content، via availability of macro- and- micro nutrients، especially nitrogen، even when different levels of Cr3+ heavy metal existed in the growth medium. The obtained correlation coefficients indicated a significant positive relationship between most physiological traits، and the highest correlation coefficient (0. 84) was found between proline and soluble carbohydrates. It appears that organic compounds، such as manure، have considerable potential to reduce the harmful effects of Cr3+ heavy metal and improve physiological indices of basil.

Air pollution is one of the most serious environmental problems in Golestan. Each day, a tremendous amount of toxic and fatal gases, different kinds of pollutants, perilous floating particles produced by cars, factories, industrial workshops, power plants and residential buildings are added to the air. Largely due to the increasing demand for cement, cement production industries around Golestan have increased their daily production. Toxic gases and hazardous particulates that release into environment from cement factories have considered as the environmental problems. Cement factory emissions into air cause serious air pollution and affect the plant and animal life in the environment. This study characterized the levels of heavy metals of the leaves near a cement factory. The concentration of eight se lected heavy metals(Ag, As, Cd, Co, Cr, Cs, Bi, Ba) were measured on 24 leaves from Quercus and Paliurus spina and Phragmitesaus tralis samples (1-2 m) collected near a cement plant via inductively coupled plasma/mass spectroscopy (ICP-MS). SPSS whit 0.05 was used for statistically data analyzing. Results showed The average concentration of heavy metals from Quercus and Paliurus spina and Phragmitesaustralis samples Were not statistically significant in other words, the absorption is the same. There is a significant difference in the cd with Paliurus spina. In other words, Paliurus spina absorb cadmium from others. The concentration of toxic metals in the leaves of plants studied area of heavy metals in the air and leaves the adsorption.

To investigate the effect of cadmium (Cd) on some physiological parameters of safflower genotypes a 3-replicate factorial randomized complete blocks experiment was conducted under hydroponics culture in greenhouse facilities of the Isfahan University of Technology in 2012. Four levels of Cd (control (0)، 1. 5، 3. 0 and 4. 5 mg/L) were tested on six safflower genotypes (Arak2811، Kouseh، Nebraska-10، C111، K12 and S149). Physiological characteristics such as leaf number/plant، leaf fresh weight، shoot fresh weight، root volume، shoot dry weight، root dry weight and Cd concentration in plant roots and shoot were measured. Results indicated that in higher Cd concentrations in the solution، negative effects of Cd on different plant traits were more visible. Safflower plants showed 61. 2، 37. 5 and 71. 8 percent decrease in their root length، leaf number/plant and root volume، respectively، and 52- and 156-fold increase in their shoot and root Cd concentration، respectively، when grown under the 4. 5 mg/L of Cd in the solution، compared to the control. Cd concentration of plant shoot was profoundly lesser than that of plant roots for all studied genotypes. It was concluded that while Cd leads to considerable changes in growth and physiological attributes of safflower، but some differences exist among safflower genotypes.

Portulaca oleracea L. belongs to Portulacaceae order. From the physiological point of view, P. oleracea L., generally known as ragwed, has a very high tolerance and compatibility to the environments polluted with salts or heavy metals. It is considered as a suitable species in planting as well as in refining soil and environment from these kinds of stresses. According to these contexts and in order to show the effects of two heavy metals, lead and copper, on malondialdehyde, proline and antioxidant enzymes activity some experiments were performed in a completely randomized design. For this purpose, plants were planted in sterile bed, leca, irrigated with Hogland nutrient solotion. Treatments included different concentrations of Pb (NO3)2 and CuSO4.5H2O (0, 10,50,100,500,1000 and 1500 µM) with three replications. After ten days of treatment, plants were harvested for experiments. According to the results, an increased absorption of lead and copper was recoreded at high concentrations compared to the control treatment. The amount of atomic absorption of these two elements in roots was more than that of shoots. Stress increased significantly at high concentrations compared to the control. The amount of proline showed a significant upward trend in both lead and copper stress as well as in both roots and shoots. Peroxidase activity showed a significant upward trend in both lead and copper stress as well as in both shoots and roots while a significant downward trend was recorded for catalase activity. In general, a higher amount of malondialdehyde, proline and peroxidase activity was obtained in root samples comperd to shoot samples, indicating the role of root as the main accumulator of heavy metals, lead and copper. The lower catalase activity in roots compared to shoots proved enzyme sensitivity towards high Pb and Cu accumulation in roots.