جستجوی مقالات مرتبط با کلیدواژه « ریشه مویی » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

Valeriana officinalis from the valerianaceae is a precious medicinal plant has been employed for the treatment of mental ailments. The study was a factorial experiment in a CRD including bacterial strains (R1000, LBA9402, C58, A4, GM, and ATTCC15834), age of explants for inoculation (10, 20 and 30 days) and different explants of valerian (leaves and roots) in three replications. Solid Manitol Yest exteract Agar (MYA) medium was used for bacterial culture and MYA medium containing rifampicin antibiotic (50 mg/l) was used to prepare the bacterial suspension for inoculation. Two inoculation methods (floating and injection method) were evaluated on the percentage of rooting and the injection method was not effective in hairy root induction on explants. After determining the best strain and the best explants of the clones were cultured in MS, 1/2 MS and MS+0.2 mg/L NAA solid culture medium it was observed that the roots placed in MD medium were the most they had biomass. Strains of ATCC15834 and A4 on the MYA medium on 30 days old leaf explants was the best strain than others. The optimized root length and the top hairy root number were induced by A4 strain. The newly produced hairy root showed the highest growth on MS medium enriched with 3% sucrose at 25°C. Our results showed that the application of ATTCC15834, R1000, LBA9402 and A4 strains induced hairy roots in valerian and the other two strains were ineffective.

Silybum marianum L. from the Asteraceae is an annual or biennial plant widely distributed in the north, west and south of Iran. The fruits are containing medicinal flavonoids such as silymarin. This study was conducted to optimize the growing conditions for hairy roots and to assay the diverse factors affecting the induction and growth of hairy roots induced by Agrobacterium rhizogenes. The seeds were cultured on MS medium and were kept in the growth chamber. After germination and seedling growth; explants from the leaves, hypocotyl and roots were taken behind growth and after 5, 10 and 15 days. The explants were inoculated (treated) with two (R1000 and 15834) Agrobacterium rhizogenes strains. Furthermore, the effects of two inoculation methods (floating and injection) were assayed on the hairy roots emergence percentage. MS and 1/2 MS growing media were compared regarding the establishment of the hairy roots in the liquid medium. In a separate experiment, the effect of sucrose amount (20, 30 and 40 gl-1) were evaluated on hairy root induction and the roots emergence percentage was recorded. To verify the transgenic identity, PCR test with specific marker genes was carried out. The results revealed that 15834 strain was the strain of choice in 5 days old hypocotyl explants and produced more hairy roots than other strain. The best rooting percentage belonged to leaf explants at 15 days-old, treated with R1000. Floating was the method of choice for inoculation. 30 gL-1 sucrose was the best treatment for the root induction as well.