جستجوی مقالات مرتبط با کلیدواژه « تجزیه فیلوژنتیکی » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

The biosynthesis pathway of fatty acids is one of the important pathways in the body of most organisms that an enzyme Acyl carrier protein (ACP) plays an important role in it. The purpose of this study is phylogenetic and in silico analysis of gene ACP. More analysis indicate that ACP gene has 4 exons, 3 introns and and 2 mRNA in prokaryotes and 13 exons, 12 introns and 9 mRNA in eukaryotes. This protein has target mitochondrial in plant eukaryotes and non-mitochondrial target in prokaryotes and it is not also included of secreted proteins. The results of multiple alignments by T-Coffee server showed that the ACP genes between bacterial species are more protected than plant species. Phylogenetic analysis of ACP proteins in prokaryotes is revealed that except for a cluster, in other case Gram-positive bacteria are in one cluster and gram-negative bacteria are in another cluster. In eukaryotes, different plant species are scattered in different clusters. These results indicated that clustering in eukaryotes are not relate to species. In addition, the study of ACP proteins in eukaryotes and prokaryotes revealed that both eukaryotes and prokaryotes are placed together in some clusters that is due to the similarities of sequences in species. Comparison of the secondary structure of the protein in eukaryotes and prokaryotes showed that the number of alpha and beta sheets in prokaryotes are more than of eukaryotes. Three-dimensional modeling of this protein was done by homology modeling using Swiss Model database in wheat (as representative of eukaryotic) and bacteria Clostridioides difficile 630 (as represented prokaryotes). The best templates were extracted with high similarity from PDB database. To validation of modeled structure and esterochemical analysis, Ramachandran plot was drawn and dihydral angles were calculated. Structural quality evaluation results showed that the proposed models are good quality and stability. The study of protein structure may help to understand protein function and the details of its structure can be useful in studies of the active site of the protein and docking.

Maize dwarf mosaic virus (Maize dwarf mosaic virus) is one of the most important and widespread maize potyviruses in the world. In order to determine the taxonomic position of the isolates of the virus in Golestan province among cereal potyviruses and other strains of MDMV and its relationship with strains of other countries, corn and johnsongrass plants with mosaic symptom and dwarfing were collected from fields of Golestan province. Samples were verified using an antiserum against MDMV by indirect ELISA. The viral RNA was isolated from an infected sample and the 3΄-region of the genome of virus was amplified using potyviral degenerate and specific primers by RT-PCR.The obtained consensus sequence for CP-UTR region was 1126 nucleotides. Comparison was made between these isolates and a number of other cereal potyviruses available in GenBank and phylogenetic tree was constructed by neighbor-joining majority- rule method. In phylogenetic analysis 46 sequences were grouped into 6 viruses: SCMV, PenMV, IJMV, SrMV, ZeMV and MDMV. In this tree, isolate of Golestan were grouped with isolate of Bulgaria. Based on this analysis the Iranian isolate of MDMV has been probably originated from Europe or more probable from Mediterranean basin