جستجوی مقالات مرتبط با کلیدواژه "رتروترانسپوزون" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

This experiment was conducted in order to assess genetic variation and determination of the best genetic structure and the grouping of safflower genotypes using ISSR and retrotransposon markers and different agricultural traits. In this study, 28 safflower genotypes were evaluated using 7 ISSR markers, 3 retrotransposon markers and 12 combined ISSR and retrotransposon markers, as well as eight different morphological traits with different statistical methods. From 22 primers, 117 polymorphic bands were created. The primers, primer-of UBC-827 and TOS-1 with 13 band maximum number of bands, and primer UBC-811 and UBC-822 in combination with 5-band TOS-1 with the lowest band for ISSR primers and Retrotransposons. The average percent polymorphism obtained in this study for markers ISSR, Retrotransposons and primer combinations was from 38.46 to 88.88 percent ISSR and Retrotransposons is variable and the mean percentage of polymorphic for this is equal to 62. High standards of straw gene diversity, Shannon index, the PIC and the number of effective allele primer UBC-810, UBC-811 and primer combination TOS-2 + HB-12, TOS-1 + HB12 and TOS-1+ UBC822 show performance Primers on the assessment of genetic diversity in this article. The morphological traits of 28 genotypes by UPGMA and Euclidean distance criteria were divided into 4 groups. Grouping results of cluster analysis by linear discriminant analysis using Fisher's focal 82.1 percent for morphological traits were confirmed. Principal component analysis showed that the main vectors of the first and second respectively are 8.22 and 6.84 and the 10 first components validated 63.47 percent of total variance. Mantel test the relationship between molecular and morphological data matrix equivalent 0.214 that show little correlation between the two data. Overall, the results showed that there is a considerable genetic variation in safflower germplasm that can be used to select parents and desirable genotypes in safflower breeding programs.

In this study, geneti diversity of 28 genotyped of rapeseed using 7 markers ISSR, 3 indicates Retrotransposons and 22 combined markers were evaluated. The results of cluster analysis and molecular evaluation UPGMA with method by criteria simple matching coefficient using data from primer ISSR, Retrotransposons and hybrid primer ISSR and Retrotransposons 28 genotypes studied in 3 groups, the groups were: 14, 9 and 5 genotypes are among the groups, the first group is the largest group, but the morphological traits of 28 genotypes by UPGMA and Euclidean distance criteria were divided into 4 groups. Grouping results of cluster analysis by linear discriminant analysis using Fisher's 100 percent for molecular.

In order to mapping additive and epistatic QTL and their interaction with environment for traits related to spike characteristics using a RILs population of wheat, comprising 148 recombinant inbred lines derived from a cross between two winter wheat cultivars, ‘YecoraRojo’ and ‘No. 49’, was evaluated in two locations in Iran (Miandoab and Mahabad) during 2014-2016. A linkage map including 177 microsatellite and 51 retrotransposon markers was used in this study. Quantitative trait loci (QTL) were determined using QTL Network 2.0 software based on the CIM and mixed-linear method. In the present study, the highest broad (58.31%) and narrow-sense (29.15%) heritability was measured for spikelet number per spike and the lowest broad (51.28%) and narrow-sense (25.64%) heritability was detected for spike length. Results of QTL analysis showed that in normal condition, one QTL (R2A= 1.54%), one QTL×E (R2AE= 4.40%), 2 additive × additive epistatic effects (R2AA= 0.44- 0.4%) and 6 QTL × QTL×E interactions (R2AAE= 8.24-9.7%) were significant. In water deficit condition, 1 additive × additive interactions (R2AA= 4%) and 3 QTL × QTL × E interactions (R2AAE= 6.98%) were identified. In average of two conditions, two QTL (R2A= 0.78%), 1 QTL×E (R2AE= 5.15%), 10 additive × additive epistatic effects (R2AA= 0.02-7.9%) and 14 QTL × QTL × E interactions (R2AAE= 0.86-8.92%), were significant which can be due to the high number of QTLs with low effects and also environmental effects.

In order to Mapping main and epistatic effects and environmental interactions of QTLs for Phenological traits in normal and water deficit conditions in wheat, a recombinant inbred lines population, comprising 148 lines derived from a cross between two winter wheat cultivars, ‘YecoraRojo’ and ‘No. 49’, was evaluated in two location in Miandoab and Mahabad during 2014-2016. A linkage map including 177 microsatellites and 51 retrotransposons markers. Analysis of variance results showed that there was a significant difference between genotypes for all studied traits. QTL analysis based on composite interval mapping (CIM) showed in normal moisture conditions 2 QTL, 2 QTL × environments interactions, 14 additive × additive epistasis effects and 10 QTL × QTL× environment effects were significant. Phenotypic variance explained by main QTL varied from 2.6% to 7.14% for grain filling period and days to heading. In water deficit condition, 6 QTLs, 2 QTL × environments interactions,9 additive × additive inactions and 2 QTL × QTL × environment interactions effects were found, The highest and lowest explained phenotypic variation belonged to photosynthetic for grain filling period and days to heading with R2 value of 4.46 and 12.44 respectively. Also for days to heading and days to physiological maturity QTLs was detected on chromosomes 2A, 3A, 5A and 2B which coincided with Vernalization-related genes (VRN) and genes sensitive to photoperiod (Ppd) chromosomes in wheat.

The aim of this study Investigating the genetic variation in the collection germplasm Sistan grapevine native and non-native cultivars using REMAP marker. To study the genetic diversity of grapevine native and non native cultivars of Zahak research station in Sistan, 33 grape varieties (Vitis vinifera L.) were evaluated by by 7 primer combination REMAP combination ISSR primers and retrotransposons based primer from of Ty1-copia and Ty3-gypsy families. The observed polymorphism for markers MS1 / Gret1 Ra, MS5 / Gret1Rb in both native and non-native varieties were 80 and 75%, respectively. The results revealed that the Gret1 retrotransposon has more transcription and translocation trough the genome. Cluster analysis based on Jaccard's similarity and UPGMA algorithm showed that the population is divided into two groups at (0.44) similarity; one group with twelve sub-groups and one group with one varieties. The results of principal component analysis extracted six components that explained 50.44 % of the total variation among the studied population.