جستجوی مقالات مرتبط با کلیدواژه "ریشه ای مویین" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

Galega (Galega officinalis L.) from the leguminous family is used to treat diabetes. Hairy root culture is a valuable system for the production of secondary metabolites on a large scale. The use of elicitors in plant tissue and cell culture is one of the most efficient biotechnological tools for inducing biosynthesis and accumulation of secondary metabolites. This study aimed to investigate the effect of iron, silver, and silicon nanoparticles on growth and galegine content in hairy roots of G. officinalis.

In this study, induction of hairy roots in leaf, cotyledon, and hypocotyl explants of G. officinalis was performed using the A4 strain of Rhizobium rhizogenes, and the effect of different concentrations of silver, silicon, and iron oxide nano-elicitors on growth and galegine, phenol, and flavonoids content of hairy roots was investigated.

The highest rate of root induction was obtained in leaf explants with an average of 5 roots per explant. The highest root growth rate belonged to the treatments with 50 and 100 mg l-1 silicon nanoparticles for 72 h. Application of all levels of silver, iron, and silicon elicitors for 36 h resulted in a significant increase in the total phenol content of hairy roots. Thus that the highest amount of total phenol was obtained in 36 h treatment of roots with a concentration of 100 mg l-1 of silicon nanoparticles without significant difference with 10 and 20 mg l-1 silver nanoparticles. Also, the highest amount of total flavonoids was obtained at 200 mg l-1 iron nanoparticles for 36 h; and the highest content of galegine was obtained in the treatment of roots with 10 and 20 mg l-1 silver, and 100 mg l-1 silicon nanoparticles for 36 h.

The appropriate type and concentration of elicitors is an effective factor in the response of hairy roots to nano-elicitors. The highest yield of galegine (17.55 mg) was obtained in the treatment of roots with 50 mg l-1 of silicon nanoparticles for 72 h. This can be attributed to both better growth of hairy roots and high galegine content at this treatment.

Induced hairy roots by Agrobacterium rhizogenes are a suitable tissue for the production of secondary metabolites, due to the stability and high production of roots in hormonal-free conditions. Trigonella foenum – graecum L. is one of the medical plants in the family Fabaceae. Many useful components in Trigonella foenum L. leafs are Calcium, Iron, Phosphorus, Carotene, Ascorbic acid, Protein, Thiamine, Riboflavin. Also, this plant widely used as anti-diabetes, blood glucose, cholesterol- lowering, Anti-cancer, Antibacterial, and food stuffing. In this study, hairy roots were induced using A. rhizogenes strains A4, ATCC11325 and ATCC15834 by injection method in complete seedling, cotyledon and hypocotyl explants. The effect of strain type and explant type, were investigated on the effectiveness of hairy roots. The explants were cultured in B5 medium with 4 replications. Hairy roots appeared in bacterial inoculation after 2 to 3 weeks. The highest amount of transgenic (93%), hairy roots number (10.43 number) and the maximum root length (6.64 cm) were observed in complete seedling explant of 10 days and A4 strain. In the hypocotyl explants, the hairy roots were not observed.

Hairy roots culture is a potential system for secondary metabolite production in medicinal plants. In this research, hairy root induction in Echinacea angustifolia was established using Agrobacterium rhizogenes. The effects of different A. rhizogenes strains (AR15834, A4, K599 and MSU440) in two solid growth regulator- free culture media, half strength MS salts with B5 vitamins and WPM, were evaluated on hairy root induction in 60-day-old explants. The highest percentage of root induction was obtained by AR15834 strain in the WPM medium (46.6%). Also, the effects of age (20, 40 and 60 day-old) and type (leaf, leaf lamina and petiole) of explant on hairyroot induction were investigated by AR15834 strain in the WPM medium. The high frequency of hairy root induction was obtained in leaf explants (52.4%) and 40 day-old leaf explants (62.3%).Transgenic hairy roots were confirmed by PCR analysis with specific primers of rolB gene.