جستجوی مقالات مرتبط با کلیدواژه "مارکر" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

The genus Thymus L. is one of the largest genera of the Lamiaceae family and aromatic plants. Plants of these genera have commercial value due to their essential oils. Thymus eriocalyx is one of the species that is exclusively on the Iranian plateau. In order to investigate the molecular characteristics of Thymus eriocalyx populations in Iran, 10 habitats were selected in Lorestan, Markazi, Hamedan, Kermanshah and Kurdistan provinces (using the DSS method) (50 plant samples). Also, 15 primers with 10 N lengths selected. The molecular data, based on RAPD experiments, divided the populations into five distinct groups. Primers used in this experiment produced 71 bands, which showed 68 polymorphic bands. OPA-05, OPA-17, OPD03 and OPE-20 primers with the highest polymorphism and marker index had excellent ability to study genetic variation in studied species. P2 and P7 populations had the maximum effective allele and p9 population had the minimum value. The highest and lowest mean of expected heterozygosity belonged to P2 and P9 populations, respectively. The highest genetic similarity was observed between P2 and P3 genotypes and the lowest genetic similarity between P2 and P10. The results of analysis of variance (AMOVA) showed that the variation within the populations of these species (68%) is greater than the diversity among populations (32%), which this fact prove the importance of single plant selection and paying attention to individual in Thymus breeding programs.

The genetic variation was studied for 13 genotypes of Lolium multiflorum using molecular and cytogenetical markers. karyotype were prepared for 5 metaphases cells in each genotype. The studying genotypes had an asymmetry karyotype and variation was significant between genotypes for karyotypic characters. The results of compare means showed that the diploid genotypes had an average chromosome length more than tetraploid genotypes. The tetraploid genotypes had the intrachromosomal asymmetry more than diploid genotypes. Karyotypic evolution for L. multiflorum was more from intrachromosomal asymmetry, considering studied genotypes and intrachromosomal asymmetry increased by development of ploidy level. The genotypes of 390, 374, vi and plc had the most karyotypic evolution and the genotypes of 1624 and 1765 had the lowest karyotypic evolution. Genetic variations for studying genotypes were surveyed using 12 ISSR primers, that the number of nine primers can be scored. The ISSR primers can be produced the number of 66 bands, which the polymorphisms were showed for the number of 56 bands. The primers of IS9 and IS12 showed the highest number of band (10 bands) and IS15 showed the lowest number of band (3 bands). A desirable polymorphism between genotype observed based on ISSR markers, which the primers of IS9, IS10, IS13, IS14 and IS16 were determined for genetic variation study in Lolium multiflorum as desirable primers. The tetraploid and diploid genotypes separated based on surveyed primers. The diploid genotypes of 393 and 374" merely were grouping with tetraploid genotypes. Grouping of genotypes based on markers don’t agreement with geographical origin of genotypes.