جستجوی مقالات مرتبط با کلیدواژه "chitosan" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

Salinity is one of the most important stresses that reduce the yield of most plants. Plants use different mechanisms in response to environmental stresses. Chitosan and its oligomers are used in plants to resist abiotic stresses such as salinity. In this study, the effect of chitosan and salinity on gene expression and p5cs enzyme activity and proline content in rapeseed was investigated.

For this purpose, rapeseed plants were treated with sodium chloride solution (0, 50, 100 and 150 mM) and chitosan (0, 5 and 10 mg/l). The experiment was performed as a factorial experiment with a completely randomized design in 3 replications. Treated plants were harvested to measure gene expression, p5cs enzyme activity and proline content.

With increasing salt concentration, the expression of delta-1 proline-5 carboxylate synthetase (P5CS) gene, enzyme activity and proline content increased. In the combined salinity of 100 mM with chitosan 10 mg/l, gene expression, activity and proline content in rapeseed had the highest amount. The use of chitosan in salt-containing medium compared to salinity treatments in same concentration, caused more P5CS gene to be expressed, increased enzyme activity and subsequently more proline was synthesized. Therefore, there is a positive correlation between gene expression, enzyme activity and the amount of proline produced.

According to the results, chitosan with a concentration of 10 mg / l in salinity treatment, by increasing gene expression and the activity of the enzyme delta-1-proline-5-carboxylate synthetase (P5CS), produced proline, which increases the plant's resistance to salinity stress.

Di-acetylated chitin derivative of is Chitosan that there is a biologically degradable cationic polymers; This natural polymer in the past to recover heavy metals from sea water and industrial water and diesel oil were used. Present of Metals in the oil is importance because of their impact. Some metals, such as copper and nickel as the catalyst and reducing oxidative reactions are known thermal stability of oil and therefore very little oil concentration of these metals can tolerate. The purpose of this research was to investigate the removal of heavy metals, iron and copper from soybean oil use to Commercial chitosan and is Best of Chitosan concentration and the reaction temperature. Removal of metals by adding Chitosan crude soybean oil at temperatures of 25, 45, 55, and 65 ° C act for 1 h and Measuring the atomic absorption efficiency of Chitosan most suitable reaction temperature and concentration were investigated. Removal efficiency is dependent on several factors such as the amount of chitosan , the temperature and time. The results of obtained can use to chitosan for the removal of heavy metals from edible oil and prevent oil degradation during production and storage According to its low cost relative to the types of adsorbents. According to the results, it was observed that increasing the chitosan concentration increased metal removal rates .

Glucoraphanine is an aliphatic glucosinolate, which largly found in Lepidium draba from Brassicaceae family. This glucosinolate convert to isothiocyanate, sulforaphane (SFN) in the presence of myrosinase which has different biological activities such as the antioxidant and anti bacterial properties, and also able to prevent proliferating of cancer cells.  CYP79F1 is the first enzyme in the biosynthesis pathway of glucoraphanine.

 In this study, L. draba seedlings wer grown for 7 days in the presence of different concentration of sucrose and chitosan (0, 25, 50, 100, 200 and 400 mg/L), in fully-random designs with three repetitions. After collection of the seedlings, SFN content in the arial parts of them were measured using HPLC apparatus. Furthermore, in the treated seedlings, the gene expression level of CYP79F1 were examined using the Real Time PCR technique.

 The results showed, that SFN content in the sucrose treated seedlings significantly increased compared to the control. While in the chitosan treated seedlings significant increase in SFN content was only seen at the 200 mg/L concentration. The results showed that the expression of CYP79 F1 was significantly increased in the treated seedlings with 50 mg/L sucrose and 50 and 100 mg/L chitosan compared to the control.

 According to the results, it concluded that the mentioned elicitor's concentrations can stimulate aliphatic glucosinolate biosynthesis through expression of CYP79F1 gene. Based on the role of SFN as a valuable medicinal metabolit and also the role of CYP79F1 enzyme in the glucoraphanin biosynthesis, it suggested that the effects of others concentrations and time of treatment of the mentioned elicitors were analyzed on this plant.