جستجوی مقالات مرتبط با کلیدواژه "hypericum perforatum" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

For many years, different species of the genus Hypericum spp. used to treat neurological disorders and depression. The high susceptibility and fluctuation of the production of secondary metabolites caused by climate changes in this plant can be controlled by in vitro culture systems. Hairy roots (transgenic) has been used as an alternative for production of medicinal plants to produce secondary plant metabolites in many species, but the production of these roots have always faced challenges, one of them is the difficulty of induction. Therefore, the present study aimed to introduce practical guidelines for induction of adventitious roots from Hypericum perforatum leaf explants treated with different concentrations of indole-3-butyric acid (IBA) and different light sources including red, far red, full light, blue and flourcent , as well as absolute darkness as control treatment. The results of this study showed that the concentration of 2 mg/l IBA under dark treatment had the best inductive effect on adventitious root production from leaf explants. Higher concentrations of IBA caused callus formation in both light and dark conditions. In investigating the effect of light source on the frequency of adventitious root induction, the highest number of roots was recorded in far red and red, while the lowest number of adventitious roots was recorded under full light treatment. The highest level of hypercin was in the dark followed by fluorescent light and the lowest in blue light, but different light sources did not show a significant effect on hyperforin content.

miRNA genes consider as an important class of gene expression regulators that have diverse responses to environmental stresses and play a key role in regulating gene expression during post-transcriptional regulation. Hypericum perforatum, is well-known medicinal plant for its application in the treatment of depression due to the effects of its bioactive compounds named hypericin and hyperforin. The present study was performed to identify the conserved miRNAs and their target genes in the transcriptome of H. perforatum. First, RNA-seq data were obtained from the European Nucleotide Archive (ENA) of EMBL-EBI database and then the transcriptome (RNA) sequences were assembled. The non-coding transcripts were identified and considered as miRNA precursors candidate sequences. Finally, six miRNAs named Hp-miR395, Hp-miR845d, Hp-miR414, Hp-miR159, Hp-miR159e, and Hp-miR156c were identified from the candidate sequences using of C-mii software after applying strict filters.  Investigation of the protein-protein interaction network determined the relationships between the target genes and target proteins especially in transcription factors. In the next step, to confirm the target genes for the identified miRNAs, the foliar application of Methyl Jasmonate (MJ) with concentrations of 0 (control) and 200 μM was performed on H. perforatum plants and the expression pattern of two target genes was investigated. In the analysis of qRT-PCR expression changes at 12, 24, 48 and 72 hours after applying MJ, the relative expression level of Hyp-1 (DN121523_c1_g4_i2) and HD-Zip (DN121003_c0_g1_i1) transcripts increased after 72 hours of application of MJ. In general, regarding to the regulatory role of the identified miRNAs in the present study, these genes could be used to better and more accurately identify the biosynthetic pathway of hypercin and hyperforin.

St. John’s wort belongs to Hypericaceae family and has been used since ancient Greeks as a herb for the treatment of anxiety, depression, wounds and burns. In present study, the effects of different concentrations of CPPU and BA (each in three concentrations) in combination with two concentrations of IAA on callus production and shoot regeneration of Hypericum perforatum from leaf and stem explants were evaluated to identify the best growth regulator combination for obtaining the highest yield of in vitro regenerated shoots. This was implemented in a CRD-based factorial experiment with three replicates and five samples in each replicate and the traits including callus fresh weight, number of shoots, shoot length, length of the tallest shoots, and the number of nodes of the tallest shoots were measured and examined. Results showed that the most effective combination for regeneration was 1.5 mg/l CPPU and 1 mg/l IAA, which may be a proof for more effectiveness of CPPU in comparison with BA as cytokinin source on shoot regeneration in Hypericum perforatum. The use of 1 mg/l IAA in combination with 1.5 mg/l CPPU or BA resulted in a significant increase in shoot regeneration. It was also showed that 1 mg/l IAA in combination with 0.5 mg/l BA could cause a significant increase in callus fresh weight.

For investigating the effects of salinity on the germination and growth of five medicinal species, an experiment carried out in 2010. Five medicinal species including Hypericum perforatum, Foeniculum vulgare, Cuminum cyminum, Anthemis persica and Achillea millefolium were analyzed at four levels of salinity (0, 50, 100 and 150 mM of sodium chloride in Petri dish) using a completely randomized, factorial design with four replications. The traits under analysis included the percentages and rate of germination, the length of root and shoot, the length of seedling, S/R (shoot/root), the fresh and dry weights of the seedling, and the vigor index. The statistical analyses at germination and seedling growth levels indicated that an increase in salinity significantly decreased all of the traits at P<0.01 and S/R at P<0.05 in the five species under analysis. The highest percentage and rate of germination belonged to Anthemis persica and Achillea millefolium, and the lowest effect of salinity on the percentage and speed of germination was observed in 50 mM and control concentrations. The obtained results regarding the length of root, shoot, and seedling indicated that this decrease was more in 50 and 150 mM concentrations in comparison to 100 mM one. It must be noted that the 100 mM salinity level had the lowest amount of S/R.

Hypericum perforatum L. is a medicinal plant abounding with secondary metabolites which have clinically proven anti-inflammatory, antiviral, antifungal, and antidepressant activities. In this study, the genetic diversity of 29 accessions from eight populations collected from eight provinces (Azerbaijan, Gilan, Tehran, Semnan, Kurdistan, Lorestan, Golestan and Ardabil) were assessed using Inter Simple Sequence Repeat (ISSR) markers. Twelve primers containing different simple sequence repeats (microsatellite) were used. The primers produced between 11 to 26 bands. Totally the markers produced 221 amplification products, out of which 196 bands were polymorphic. The results showed that polymorphism information content (PIC) of primers was 0.37. Primers 841Y-UBC and UBC-807 had the highest PIC (0.41). Cluster analysis based on Jacquard’s similarity coefficient using Unweight Pair Group Method with Arithmetic mean (UPGMA) indicated wide range of diversity across the studied accessions. The highest genetic distance was observed between 23335 (Ardabil) with 17982 and 14206 (both of West Azerbaijan) accessions and between 133337 and 17982 accessions (Gilan and Azerbaijan) revealing closer genetic relationship. The least genetic distance was observed between 13180 and 13347 from Gilan. Molecular variation within and among the populations (within and among provinces) were estimated 78% and 22% of total variance, respectively. The distribution reflects high genetic diversity between the accessions in each geographical area. The results revealed that ISSR markers could be efficiently used for genetic differentiation of the St. John’s Wort accessions.