جستجوی مقالات مرتبط با کلیدواژه "lepidium draba" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

Glucoraphanine is an aliphatic glucosinolate, which largly found in Lepidium draba from Brassicaceae family. This glucosinolate convert to isothiocyanate, sulforaphane (SFN) in the presence of myrosinase which has different biological activities such as the antioxidant and anti bacterial properties, and also able to prevent proliferating of cancer cells.  CYP79F1 is the first enzyme in the biosynthesis pathway of glucoraphanine.

 In this study, L. draba seedlings wer grown for 7 days in the presence of different concentration of sucrose and chitosan (0, 25, 50, 100, 200 and 400 mg/L), in fully-random designs with three repetitions. After collection of the seedlings, SFN content in the arial parts of them were measured using HPLC apparatus. Furthermore, in the treated seedlings, the gene expression level of CYP79F1 were examined using the Real Time PCR technique.

 The results showed, that SFN content in the sucrose treated seedlings significantly increased compared to the control. While in the chitosan treated seedlings significant increase in SFN content was only seen at the 200 mg/L concentration. The results showed that the expression of CYP79 F1 was significantly increased in the treated seedlings with 50 mg/L sucrose and 50 and 100 mg/L chitosan compared to the control.

 According to the results, it concluded that the mentioned elicitor's concentrations can stimulate aliphatic glucosinolate biosynthesis through expression of CYP79F1 gene. Based on the role of SFN as a valuable medicinal metabolit and also the role of CYP79F1 enzyme in the glucoraphanin biosynthesis, it suggested that the effects of others concentrations and time of treatment of the mentioned elicitors were analyzed on this plant.

Lepidium drabathrough the production of anti-cancer and anti-microbial compound, sulforaphane, is considered a valuable medicinal plants.Therefore, optimization of in vitro tissue culture to facilitate genetic transformation and improvement of traits in this plant is important. This study was conducted, by cotyledon, stem and root explants inMSmedium in the form of two separate tests. Data were analyzed in a factorial experiment based on completely randomized design. In the first experiment with different concentrations of growth regulators NAA and BAP, the highest response rate, direct shoot regeneration rate and direct shoot generation were related to root explants. The highest root production rate and root generation were showed in cotyledons explants. 1 mgl-1 BAP without NAA induced the highest direct shoot generation in root explants. Cotyledons explants in 2 mgl-1 NAA and 0.5 mgl-1 BAP produced the highest Root production rate and the highest root generation. Callus induction rate and callus initiation of stem explants were more than others, but the callus regeneration rate in three studied explants showed no significant difference. The highest shoot generation from regenerated callus was observed in the cotyledons explants. The second experiment with a combination of different concentrations of 2,4-D and BAP, under the influence of two treatments darkness and 16h photoperiod was performed. The highest investigated traits was in the 16h photoperiod. Cotyledons and stem explants were showed the highest callus induction rate and leaf explants showed the greatest weight of callus; While the highest callus regeneration rate and callus growth rate were obtained from the root explants.From leaf explants, the highest callus weightwas obtained from 0.5 mgl-1 2,4-D with 3 mgl-1BAP and the most growth rate of callus was obtained from 0.5 and 1 mgl-1 2,4-D and 3 mgl-1BAP. Also the combination of 2 mgl-1 2,4-D with 2 mgl-1 BAP and 3 mgl-1 BAP, produced the highest callus regeneration in root explants.