جستجوی مقالات مرتبط با کلیدواژه "mas" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

Marker-assisted selection (MAS), a selective method which is not influenced by environmental factors. The success of MAS-based breeding programs depends on the selection and validation of the markers used. In this study, to validate the gene(s) associated with salinity stress And evaluation of allelic diversity of these markers in mutant rice lines, Band pattern of 18 SSR markers on a leaf sample of 14 mutant lines (M9) of rice, along with 2 susceptible controls (IR29 And Sepidrood) and 2 tolerant controls (Nonabokra and Dylmani) in 1398 in Genetics & Agricultural Biotechnology Institute of Tabarestan (GABIT). 11 primers were selected based on band pattern analysis in susceptible / tolerant cultivars. The molecular analysis results showed that OsMAPK4, OsCML11 and OsCPK17 had highest polymorphic information content (PIC). OsMAPK4 and OsCML11 had highest marker index (MI) at a rate of 0.23. The lowest PIC (0.05) and MI (0. 11) was accounted for OsCAX(D). Cluster analysis of molecular data, divided rice genotypes into three distinct groups. However, analysis of Biplot classified the genotypes into four different groups. In this study, 3 genes OsCML11, OsMAPK4 and OsCPK17 were identified on chromosomes 1, 6 and 7 respectively, as the most efficient primers in identifying the genetic diversity between the rice genotypes, Considering that these primers have a very high linkage with salinity resistance genes, can be predicted that 3 lines G1 (M9-P1-7-2-1), G8 (M9-P3-21-1-1) and G9 (M9-P6 -7-1-1) have high tolerance to salinity stress.

Tobacco is one of the agricultural and industrial plants which plays important role in economy and revenus of producer contries.Potato Virus Y (PVY) is one of the most Dangerous Viral diseases on tobacco plants and reduces the quality and quantity of tobacco crops. The best and most industrial method for PVY disease is production of resistant varieties. Improvement for resistance to this disease from classic methods is difficult and need many expancess.DNA markers Linked to resistance gene and have high polymorphism(Like SSR Markers) can be useful in acceleration of resistant varieties Breeding programs. This research has been done for studying the probability accesses to Marker-assisted Selection (MAS) for resistance trait to PVY disease in tobacco with SSR markers. For this aim,in Farvardin 1389, cultivation of parental seeds(VAM Variety) which resistant to PVY and (K326 Variety) as a susceptible parent to PVY were done.DNA of resistant and susceptible parents Leaves, were extracted, Separately.,and after of performance polyacrylamid electrophoresis and Gel staining by silver nitrate solution, among of them,28 pair primers have the most polymorphisms on parents,were selected and screened. Then,this 28 SSR markers were tested on resistant and susceptible Bulks to PVY disease. The final results showed that 3 SSR markers (PT20357),(PT30002),(PT20165) have the good polymorphism on resistant and susceptible Bulks to PVY.These markers can be useful to MAS for resistance or Tolerance to PVY in tobacco.