The study of probability to access Marker-Assisted Selection (MAS) for resistance trait to PVY in tobacco by SSR Markers

Tobacco is one of the agricultural and industrial plants which plays important role in economy and revenus of producer contries.Potato Virus Y (PVY) is one of the most Dangerous Viral diseases on tobacco plants and reduces the quality and quantity of tobacco crops. The best and most industrial method for PVY disease is production of resistant varieties. Improvement for resistance to this disease from classic methods is difficult and need many expancess.DNA markers Linked to resistance gene and have high polymorphism(Like SSR Markers) can be useful in acceleration of resistant varieties Breeding programs. This research has been done for studying the probability accesses to Marker-assisted Selection (MAS) for resistance trait to PVY disease in tobacco with SSR markers. For this aim,in Farvardin 1389, cultivation of parental seeds(VAM Variety) which resistant to PVY and (K326 Variety) as a susceptible parent to PVY were done.DNA of resistant and susceptible parents Leaves, were extracted, Separately.,and after of performance polyacrylamid electrophoresis and Gel staining by silver nitrate solution, among of them,28 pair primers have the most polymorphisms on parents,were selected and screened. Then,this 28 SSR markers were tested on resistant and susceptible Bulks to PVY disease. The final results showed that 3 SSR markers (PT20357),(PT30002),(PT20165) have the good polymorphism on resistant and susceptible Bulks to PVY.These markers can be useful to MAS for resistance or Tolerance to PVY in tobacco.