جستجوی مقالات مرتبط با کلیدواژه « micro propagation » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

Cultivar identification in micro-propagated date palm seedlings is laborious so that application of molecular markers to facilitate and acceleration of the procedure seems inevitable. Given the need for control the originality of micro- propagated date palm seedlings, the aim of this study was evaluation of SSR markers usability to cultivar identification in micro-propagated date palm seedlings. Original samples of Green Ghanami, Red Ghanami, Gantar, Deiry, Ostaemran, Barhi, Medjool, Zahedi and Piarum cultivars were used control. Taking into account the rigidity of leaves and subsequently high consumption of liquid nitrogen to powder leaves, an efficient method for powdering of leaves using Tissue Lyser II instrument was optimized. Eight SSR primer pairs were used for polymerase chain reaction. The Results showed that by using these molecular markers and reliable controls, determination of micro- propagated date palm cultivars is feasible. Clustering of cultivars showed that all of them were differentiated using five SSR primer pairs including mPdCIR025, mPdCIR057, mPdCIR070, PDAG1003 and DP175. Also, barcoding of scored band illustrated that c1 allele (230 to 240 bp) for Piarum cultivar and d3 allele (220 to 230 bp) for Medjool cultivar were exclusive. Totally to make the results referable, cultivar identification diagram was drawn up.

Tecomella undulata is one of endemic and endangered plant species in Iran. It is belong to  Bignoniaceae family grown in southern of Iran. Due to the specific characteristics of this plant in various aspects of medicine, ornamentation and natural resources, its mass production through tissue culture and creation of identical plants seems necessary. Effects of growth regulators on plant growth indices such as number of buds and branches, shoot length, branch greenness and rooting were studied by bud explants under in vitro conditions. The optimal aseptic technique was obtained by immersing the buds in 0.1% mercuric chloride solution for 4 minutes in spring. The best shooting was observed in MS basal medium containing BA 1 treatment and K0.5 mg/L. This treatment was the best selection for this phase with the average shoot growth of 4.9 cm and shoot length of 7.78 cm. At the rooting stage, despite the numerous treatments of the medium and hormones (various concentrations of auxin hormone), the onset of rooting was only observed in 1/2 MS IBA2.

Lettuce is considered as a model plant for biotechnology because of its compatibility with stable genetic transformation and tissue culture. GDP-mannose-3’,5’-epimerase (GME) is one of the key genes in ascorbic acid biosynthesis pathway in plants. The present study aims to transfer GME gene from Actinidia deliciosa L. into Lactuca sativa L. 

To investigate callus induction rate using the effects of explant (cotyledon and true leaves) and 16 plant growth regulator combination including concentrations of 0.02, 0.04, 0.05, and 0.1 mg/l NAA and 0.1, 0.2, 0.4, and 0.6 mg/l BAP, and also direct regeneration rate using the effects of explant (cotyledon and true leaves) and 6 plant growth regulator combination including concentrations of 0.02 and 0.05 mg/l NAA and 0.2, 0.4, and 0.6 mg/l BAP, a factorial experiment based on completely randomized design with three replications was conducted. In order to transform GME into Lactuca sativa L. using L. sativa L. cv. Setareh and Agrobacterium tumefaciens strain (C58) on two types of explants (cotyledon and true leaves), a factorial experiment with three replications and 2 min and 8 min inoculation was done.

The results revealed that the highest percentage of callus induction and indirect regeneration (100%) were observed on leaf and cotyledon explants and MS medium containing 0.1 mg/l BAP and 0.04 mg/l NAA. The results also confirmed the presence of pBI121+GME in transgenic plants.

The explant true leaves and 2 min inoculation (with 18 percent transformation ratio) were more suitable for transformation.

Supernia is one of the most important high-yield cultivars of the imported cucumber hybrids. In order to optimize asexual propagation of this variety through in vitro culture, cotyledon, hypocotyl and leaf explants were placed in the MS basal medium containing 0, 0.1, 0.2, 0.3 and 0.4 mg/l concentrations of 1-Naphthaleneacetic acid (NAA) in combination with 1, 2, 3 and 4 mg/l of 6-Benzylaminopurine (BAP) hormone. The experiment was conducted as a factorial arrangement in completely randomized design at three replications and five samples in each replicate. The percentage of callogenesis, embryogenic calli and shoot frequency of the cultured explants were evaluated. The highest percentage of callogenesis (86.6%) was obtained from cotyledon explant on the MS medium supplemented with 0.3 mg/l NAA + 3 mg/l BAP hormones. The highest embryogenic calli (61.6%) were developed on the MS medium supplemented with 0.2 mg/l NAA + 2 mg/l BAP hormones in hypocotyl culture. Also, the maximum number of shoot (20.08) were obtained on MS medium supplemented with 0.3 mg/l NAA + 3 mg/l BAP hormones in hypocotyl explant. The regenerated shoots were transferred to half-strength MS medium supplemented with 1 mg/l NAA hormone for root induction and formation. Then, the grown seedlings were adapted to the in vivo condition. Based on the results of this study, it is recommended to use the cotyledon explant for propagation and regeneration of this genotype, especially in the gene transferring studies.

Propagation of Pronus avium L. through conventional micropropagation is essential for forest development and production of scion for grafting of commercial Pronus varieties. For micropropagation and production of juvenile tissue as an initial source of proliferation, mature embryo explants were used. For in vitro shoots preparation, isolated embryos of mature trees were transferred into MS medium containing 30 and 60 g/l sucrose. There were no significant differences between MS media containing 30 and 60g/l for percentage of embryo germination using t-Student test. Factorial analysis of collected data for further shoot proliferation indicated that there were highly significant differences between the used growth regulators for in vitro shoot length and number of produced explantsýatýα=0.01ýlevel. The highest average shoot length (5.86 cm) and the highest mean number of explants production were observed on MS medium supplemented with 4 mg/l BA and 0.2 mg/l IBA. Plantlet at 4 to 5 cm in height were rooted in MS hormone free medium and after successful acclimatization transferred to a green house.

Zhumeria majdae is one of the most important aromatic plant species from Lamiaceae family. The valuable species has medicinal and industrial applications. It is an endemic and endangered species of Iran. Therefore, tissue culture techniques are proposed for propagation and conservation purposes of the species. Usual regeneration of the species is by seed. Apical buds of seedlings from Hormozgan genotypes were sterilized by HgCl2 0.1% solution for 5 minutes during spring season. The best medium for shoot proliferation was WPM containing BA (0.5 mg/l), 2iP (0.3) and IBA (0.1 mg/l). The highest rooting of the shoots was obtained by WPM supplemented with 1 mg/l of IBA. The plants were acclimatized in greenhouse after transferring in soil. The protocol of this research could be used for culture and developmental programs of the species in its original habitat.

Red nightshade (Solanum alatum Moench.) is one of the important medicinal plants from the Solanaceae family. Tissue culture technology is a very useful application of biotechnology which can be used for plant preservation, rapid multiplication and in genetic engineering for molecular breeding or improving secondary metabolite production. In this study, plant regeneration from three different explant sources derived from young seedlings, including hypocotyl, coleoptile and leaf segments, was evaluated. In this research, efficient protocols for regeneration of red nightshade from all three explants are reported. The best shoot regeneration response (100%) was obtained at 0.5 mg/l BAP concentration for leaf explants. Regenerated plants adapted to greenhouse conditions with a high efficiency (100%) and produced normal flowers and seeds. Also, leaf explant was found to be the most efficient explants for plantlet regeneration, with a mean number of 29.93 shoots per explant. Finally, the potential of leaf explants for gene transfer and expression by means of a gene gun (at two rupture disk pressure of 1100 and 1350 psi), was evaluated using transient GUS expression, and leaf explants were found to be suitable for this purpose.

Jatropha curcas is a tropical fast growing tree from euphorbiaceae family. The valuable species has medicinal، industrial and ornamental applications. Regeneration of the species was performed by seeds and cuttings. Regarding Jatropha''s seed nature (oily seed)، for which low germination is resulted، regeneration of the species was studied by stem cuttings. For micro propagation، apical buds of 2 and 4 years old genotypes̉̉̉ (Gen 1 and Gen 2) from different seasons were applied. The best treatment of sterilization was found to be HgCl2 0. 1% solution for 10 minutes on fall samples for genotype 2. The best medium for shoot proliferation was MS medium with BA (3 mg/l) and IBA (0. 1 mg/l). Effect of genotype on shooting was nonsense but genotype 2 had higher effect on growth factors. The most rooting of shoots was observed in modified MS medium supplemented with 1 mg/l of IBA. Plantlets were acclimated in greenhouse after transferring to soil.