جستجوی مقالات مرتبط با کلیدواژه "myostatin" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

Myostatin, also called growth and differentiation factor 8 (GDF8), acts as a negative regulator of muscle growth and development; Therefore, its role in animal growth and meat production is very important. Functional studies showed that a single nucleotide polymorphism in the 3'UTR region of this gene causes the elimination of its growth inhibitory role and finally the occurrence of double - muscled sheep. The tetra primer ARMS PCR method is an easy, fast, reliable and cost-effective method for the detection of nucleotide polymorphism. The present study was conducted with the aim of identifying single nucleotide polymorphism of myostatin gene (g+6723G>A) using tetra primer ARMS PCR technique in 86 Karakul sheep. Polymerase chain reaction was optimized using 3 control samples including 1 homozygous wild-type, 1 heterozygous and 1 homozygous mutant genotype of sharole breed sheep. All the studied Karakul sheep had the G allele, and the mutant type allele (A allele) was not observed, so the frequencies of the mutant type (A) and the wild type (G) alleles were 0 and 100%, respectively. To evaluate the accuracy of tetra primer ARMS PCR technique in this locus, all tested samples were also genotyped by PCR-RFLP method. The results indicated that two methods are the same as therefore using Tetra Primer ARMS PCR method been as economical to genotyping

Myostatin also known as growth differentiation factor 8, is a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. This research had been done in order to assess the effect of DL-methionine replacement with L-methionine, and also the effects of varying dietary protein levels on Myostatin gene expression in Japanese quail.
 

This experiment was carried in the form of a 2×2 factorial with 4 treatment. Treatments contained the replacement of DL-methionine with L- methionine, and different dietary protein levels, were 24 and 20 %. After about 35 days of feeding and keeping the quails, a piece of their chest has been removed immediately and were transferred to the laboratory. Myostatin gene expression measured by using RT-qPCR technique. In this method, 𝜷-actin gene was used as a house-keeping gene to normalize the gene expression data in the quantitative real time PCR.
 

DL-methionine replacement did not significantly effect on Myostatin gene expression. Whereas, reduction in protein surface from 24% to 20 %, led to significantly increased expression of Myostatin (P<0.01).
 

The results indicated that DL methionine could be replaced with L methionine, and the appropriate level of protein was 24% in the Japanese quail diet.

Myostatin (MSTN) is a member of the transforming growth factor-β superfamily (TGFβ) and is an important negative regulator of muscle growth in vertebrates. In this study, a partial sequence of MSTN gene (intron 1), in the camel population of Iran, was evaluated. Two single nucleotide polymorphisms and one insertion or deletion (InDell) were identified in intron 1 of the myostatin gene, using sequencing method. The mutations caused, two substitutions including C to T and T to C in positions 16 and 136, respectively and one InDell of T in position 218. The mutations observed in three positions of intron 1, created three different haplotypes. These haplotypes were shared among Bacterianus and Dromedaries camel populations with no haplotype restricted to a specific region or a single camel breed. Tajima D were positive for all populations except Trod station, but it wasn’t significant.

Myostatin gene belongs to the family of transforming growth factors (TGF-β), which in growth and development and maturation and specifically is expressed in adult skeletal muscle development. In this study protein amino acid sequence of Myostatin in various species, including cow, sheep, goats, horses, pigs, chickens, turkeys, ducks, goose, Columba, rats, and humans was investigated using by Bioinformatics tools. In alignment of the final section of Myostatin protein (active form of Myostatin protein that is expressed as a dimer), high degree of conservation was observed, so that the changes fluctuation in this section than the other parts of protein sequence, the least amount possible. Secondary structures such as alpha helices, beta sheets and three-dimensional structure of Myostatin proteins using specialized sites Proteus and Phyre2 was assessed. Amino acids leucine, lysine, isoleucine and glutamic acid in cow, sheep and goat showed the same frequency as compared with poultry, such as chicken, lots more. However, the frequency of the amino acid sequence of the protein myostatin in humans and pigs was moderate. Study of phylogenetic tree showed that difference between amino acid sequences among mentioned Myostatin species lead exposure in a group of birds was isolated from mammals. Investigation of 3-D structure showed that this gene is highly conserved across species.