جستجوی مقالات مرتبط با کلیدواژه "pathogenic bacteria" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

The purpose of the present study was to isolate and identify molecularly native actinobacteria that produce biosurfactant with antimicrobial and anti-biofilm activity against some pathogenic bacteria.

initial screening was done based on oil dispersion test and the best isolate was selected. Ethyl acetate solvent was used for extraction. Antimicrobial and anti-biofilm properties were evaluated using the well diffusion method in agar and 96-well microplate, respectively. FTIR and GC-MS techniques were used for biosurfactant analysis. Finally, the superior isolate was identified using the molecular PCR method.

Biosurfactant of the superior isolate showed the highest and lowest antimicrobial effect at concentrations of 0.4 and 0.3 mg/ml against Pseudomonas aeruginosa and Staphylococcus aureus, respectively. The highest and lowest anti-biofilm effects against Escherichia coli and Pseudomonas aeruginosa bacteria were observed at concentrations of 2 mg/ml. Structural analysis of biosurfactant with FTIR and GC-MS confirmed the structure of amino acid ring with chain of fatty acids and lipopeptide type. The selected isolate was 95% similar to Streptomyces genus and B1AY species.

The local isolate of Streptomyces has a remarkable ability to produce biosurfactant with antimicrobial and anti-biofilm effects against pathogenic bacteria such as Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli and can be used in the field of pharmaceutical biotechnology.

Recently , due to the resistance of bacterial pathogens to the detection of new antimicrobials, much attention has been paid. In the present study, the antimicrobial properties of four extracts of leafy leafy leaves, henna leaves, walnut shells and pomegranate peel on three gram-positive bacteria, Staphylococcus aureus, Staphylococcus epidermidis and Bacillus subtilis, and two gram-negative bacteria of Escherichia coli and Pseudomonas aeruginosa were studied. it placed. Antimicrobial effect of the alcoholic and aqueous extract of these plants was studied by diffusion and well discarding method by measuring the non-growth aura's diameter and the minimum inhibitory concentration was investigated by ELISA method. The results show that in two methods, diffusion discs and wells in alcoholic and aqueous extracts have the highest effect on Staphylococcus epidermidis bacterium and have the least effect on E. coli bacteria. The minimum amount of MIC for alcoholic extract is 31.25 and MBC 54.25 mg / ml, which is related to Escherichia coli bacteria. The results measurements of total phenolic compounds, flavonoids and tannins also indicate that the highest amount of this product is in the concentration of 500 mg / ml of the extract of plants. Also, the amount of antioxidant activity in the alcoholic extract is about twice as much as the aqueous extract. The results of this study indicate that the combination of extracts of plants, especially alcoholic extract, has a more antibacterial effect than the aqueous extract, which can be due to the presence of more antibacterial compounds in the extract.