جستجوی مقالات مرتبط با کلیدواژه "pvy" در نشریات گروه "بیوتکنولوژی و ژنتیک گیاهی"

Potato virus Y (PVY) is one of the most damaging viruses of potato plants which infects most cultivars and causes a significant decrease in yield products quality and also economical losses annually. Recently, the application of pathogen derived resistance mechanisms has opened new horizons for the development of virus-resistant transgenic plants. This research was carried out to study RNA silencing to engineered potato plants that are resistant to potato virus Y (PVY). RNAi construct was designed from coat protein (CP) segment of PVY. Potato explants were transformed by Agrobacterium tumefaciens AGL1 with pCAMRNAiCP containing bar selectable gene. The rate of regenerated plants was evaluated in the selected medium containing the herbicide phosphinotricin after growth and propagation. Transgenic plants and their gene expression were investigated using polymerase chain reaction (PCR) and RT-PCR. Results showed that 42 percent of regenerated plants were transformed. The expression of CP hairpin construct was determined by RT-PCR analysis in most of the lines. PVY resistance bioassay showed that 82 percent of transgenic potato plants were resistance to PVY.

Potato virus Y (PVY) as one of the serious diseases on tobacco (Nicotiana tabacum L.), reduces leaf yield and its quality. Use of resistant genotypes is being considered as the most effective, economical and a safe way to reduce damages of the virus. In the present study, the response of 90 oriental and semioriental tobacco genotypes was evaluated against PVY using randomized complete block design with three replications under controlled conditions. Each replicate consisted of 6 seedlings. The leaves of each genotype were inoculated mechanically via rubbing method at 4-6 leaf stage with PVY. Four weeks following inoculation, the studied genotypes showed different severity symptoms associated with PVY. ELISA assay were applied on the inoculated plants by the indirect method using the polyc lonal antibody specific to PVY. Optical density value of each infected sample was read at 405 nm using an ELISA reader. Analysis of variance on ELISA data showed significant differences among genotypes for concentration of PVY. Results revealed that 'Erzeogovina' genotype with OD=0.68881 contained the highest virus concentration whereas the 'KB101' genotype with OD=0.0005 contained the lowest virus concentration. The differed reactions of genotypes to PVY virus encourage the use of resistance genetic resource for the reduction of disease damages.