جستجوی مقالات مرتبط با کلیدواژه « relative gene expression » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

The various medicinal functions of Yarrow have made it an important medicinal plant in medicine. Also, yarrow is a rich source of antioxidants and flavonoids that protect plants from the harmful effects of active oxygen species. The aim of this study was to investigate the effect of salicylic acid treatment on the expression pattern of two genes (aox2 and pal2) involved in the production of antioxidants and flavonoids in Yarrow plant. For this purpose, a factorial experiment was conducted in a completely randomized design including salicylic acid at two levels (0 and 50 mM) as the first factor and sampling time with two levels (24 and 48 hours) as the second factor in the greenhouse of the Faculty of Agriculture. First, the yarrow plant was subjected to hormonal treatment in Johnson's hydroponic environment, and 24 and 48 hours after the treatment, leaf tissue sampling was done. The results of qRT-PCR showed that salicylic acid stimulation significantly decreased the expression of two genes in the path of producing antioxidants in yarrow. The lowest relative expression of studied genes occurred 48 hours after treatment with salicylic acid. In general, the use of salicylic acid triggers molecular processes that result in the plant responding by changing the expression level of genes encoding antioxidants and flavonoids; therefore, it is suggested to use other stimulants such as jasmonic acid to investigate the expression of genes encoding antioxidants and flavonoids in future researches.

The yield and acceptable quality of the oil of canola make this plant as one of the most important plants for providing edible oil. Due to the hot and dry climate in Iran and water necessary for seed germination, discovery of the solution to improve germination and seedling establishment is required. Seed priming and treatment of seedlings with the extract of the seaweed Ascophyllum nodosum is one of the solutions for uniform germination and establishment of seedlings. The currently study have been explore the effect of application of Ascophyllum nodosum seaweed extract on seed germination and changes in the relative expression pattern of some important key genes related to gibberellic acid and brassinosteroids biosynthesis pathway using qRT-PCR. For this purpose, germination indexes were measured by the application of treatment 1% v/v treatment of Ascophyllum nodosum extract in four levels of treatment (0, 10, 15 and 30 days) until flowering stage. The leaf of sampling in each treatment was performed in three biological replications. The results of this study showed the effectiveness of seaweed extract treatment on increasing germination and seed vigor percentage, so that 100% germination of treated seeds obtained on the 3-day. The relative expression of algae extract showed an increase in the expression of EXP, ATI, ENTKO, AEC and BINSP genes, which are all important and as the key genes in the germination process compared to the control treatment. The use of seaweed extract treatment showed the highest expression in ATI (4.39) and ENTKO (5.70) genes in 30-day treatment, respectively. The lowest expressions for GIB and EXP genes in all three treatments (10, 15 and 30 days) were obtained. Also, there was the highest positive correlation between EEN and GIB (1), ENTKO and BINSP (0.98), AEC and BINSP (0.96) genes at the 5% significantly level. The correlation of these genes due to the use of algae extract ultimately led to a positive effect on the germination process of rapeseed seeds. Also, the results of heat map showed the genes of EXP and AEC (-0.57), GIB and ATI (-0.50) genes and XEN and ATI (-0.49) have a negative correlation at an error level of 5% and the increase of one led to the decrease of the other. This indicates that’s the activity of the final product of each of these genes is able to affect the germination process and promoting plant growth and development.

Low temperature stress is a kind of abiotic stress directly affecting growth and productivity of important agricultural products including wheat, worldwide. The purpose of the present study was to investigate the relationship between developmental regulation with low temperature tolerance, hydrogen peroxide (H2O2) accumulation and the expression of antioxidant enzymes genes in different phenological development stages in lines (RIL) of crosses between Pishtaz and Norstar wheat. Winter- and facultative-habit genotypes in the second period of low temperature acclimation, coinciding with the saturation range of the vernalization requirement, reached their maximum of low temperature tolerance, followed by a decrease in low temperature tolerance at the reproductive phase in third period of low temperature acclimation. Spring genotypes had very limited low temperature tolerance. Relative expression of TaVRT-1 gene in spring genotypes showed high and constitutively expression during low temperature acclimation periods. However, the relative expression of this gene in winter- and facultative-habit genotypes is expressed only after vernalization saturation. The relative expression of antioxidant enzymes genes were higher in winter- and facultative-habit genotypes than spring- habit genotypes, and reached its maximum in the second period of low temperature acclimation. Along with the increasing of relative expression of TaVRT-1 gene and decreasing antioxidant enzymes genes in the third period of cold acclimation, the accumulation of H2O2 reached maximum. Our results suggested that winter- and facultative-habit genotypes decreased the expression of TaVRT-1 gene and subsequently increased the length of the vegetative phase and the relative expression of antioxidant enzymes genes. Such reactions prevented the oxidative damage induced by low temperature stress and promoted more tolerant of low temperature.

Black cumin (Nigella sativa) is an important medicinal plant belongs to the Ranunculaceae which produce large diversity of secondary metabolites including industrial and medicinal monoterpenes/ triterpenes. In the present work, relative gene expression of genes involved in biosynthetic pathway of terpenes including a monoterpene synthase (MTS), geranyldiphosphate synthase (GDS), β- amyrin synthase (βAS) and squaleneepoxidase(SQE) were investigated in black cumin plants treated with methyl jasmonate. The plants were grown in a greenhouse sprayed with 0.1 mM methyl jasmonate. Samples were collected at 0, 4, 8, 24, 48 and 72 hours after applying the treatments. RT-PCR was performed by gene-specific primers after RNA extraction and cDNA synthesis. Results of semi quantitative RT-PCR showed that the expression pattern of GDS gene differed with the results of other genes. The highest gene expression of GDS was observed at 4 h and its expression remained at the same level till 8 h then dropped at next time periods. The expression patterns of monoterpene synthase, β-amyrin synthase and squaleneepoxidase showed rising trend. Gene expression analysis of the genes at transcript level using semi-quantitative PCR in black cumin leaves treated by methyl jasmonate revealedinduced-geneexpression which might show the roles of terpenes in defense mechanisms and signal transduction. Consequently, methyl jasmonate treatment can be considered to induce the terpene biosynthetic pathway and to study the enzymes/ metabolites associated with terpenoid metabolism.