جستجوی مقالات مرتبط با کلیدواژه « Vitamin E » در نشریات گروه « بیوتکنولوژی و ژنتیک گیاهی »

Immunosuppressive drugs cause destructive changes and atrophy of seminiferous tubules, decrease in sperm count and motility and sperm deformities in epididymal ducts, resulting in male infertility. Therefore, in this study, the role of vitamin A in preventing these effects was on spermatogenesis in male rats.

 In this study using 40 NMRI mice in 5 groups of 8 for 8 weeks including control group, prednisolone control group 1.5 mg/kg, prednisolone control group 2.5 mg/kg (intramuscular) And 2 groups treated with vitamin A50 mg/kg (gavage). Finally, testicular tissue and sperm parameters were examined and the obtained data were analyzed using ANOVA software.

 Prednisolone treatment had an effect on body weight, testicular weight to body weight ratio, testicular seminiferous tubules diameter and serum testosterone concentration and a significant decrease (P <0.05). It also affected other parameters including sperm motility and testicular tissue cells and caused a so-called significant decrease in surface area (p <0.05) and caused a significant decrease in other parameters such as sperm count, percentage of live sperm.

This study shows that prednisolone increases the process of spermatogenesis by increasing oxidative stress and apoptosis, and vitamin A prevents the damaging effects of prednisolone on spermatogenesis and testicular tissue by reducing oxidative stress and inhibiting apoptosis.

Lettuce is considered as a model plant for biotechnology because of its compatibility with stable genetic transformation and tissue culture. GDP-mannose-3’,5’-epimerase (GME) is one of the key genes in ascorbic acid biosynthesis pathway in plants. The present study aims to transfer GME gene from Actinidia deliciosa L. into Lactuca sativa L. 

To investigate callus induction rate using the effects of explant (cotyledon and true leaves) and 16 plant growth regulator combination including concentrations of 0.02, 0.04, 0.05, and 0.1 mg/l NAA and 0.1, 0.2, 0.4, and 0.6 mg/l BAP, and also direct regeneration rate using the effects of explant (cotyledon and true leaves) and 6 plant growth regulator combination including concentrations of 0.02 and 0.05 mg/l NAA and 0.2, 0.4, and 0.6 mg/l BAP, a factorial experiment based on completely randomized design with three replications was conducted. In order to transform GME into Lactuca sativa L. using L. sativa L. cv. Setareh and Agrobacterium tumefaciens strain (C58) on two types of explants (cotyledon and true leaves), a factorial experiment with three replications and 2 min and 8 min inoculation was done.

The results revealed that the highest percentage of callus induction and indirect regeneration (100%) were observed on leaf and cotyledon explants and MS medium containing 0.1 mg/l BAP and 0.04 mg/l NAA. The results also confirmed the presence of pBI121+GME in transgenic plants.

The explant true leaves and 2 min inoculation (with 18 percent transformation ratio) were more suitable for transformation.

Aloe Vera L. is one of the medicinal plants with different uses in pharmaceutical, cosmetic and food industries. According to increasing demand for the plant species, the most studies of tissue culture have been focused on micropropagation. But somatic embryogenesis as an appropriate way for large scale propagation, production of synthetic seeds and production of secondary metabolites have been ignored. The effect of plant growth regulators including α-Naphtalene acetic acid (NAA) and N6- Benzyladenin (BAP) and vitamin combinations including MS, LS, L2 and modified vitamin were studied, in this work. MS medium with modified vitamin including 0.4 mgl-1 Thiamine HCl plus 0.5 mgl-1 Prydoxine HCl plus 0.5 mgl-1 Nicotinic acid plus100 mgl-1 Myo-Inositol, supplemented with1.0 mgl-1NAA induced 100% callus induction. The most indirect somatic embryogenesis (50%) was induced by applying MS culture medium with modified vitamin supplemented with 0.2 mgl1 NAA and 5.0 mgl1 BAP. Eighty percent of plantlet regenerated from somatic embryos was survived after maturation, plantlet production, and acclimatization.

One of the hypotheses of cell damage after cryopreservation is the deleterious effects of oxygen free radicals generated during freezing and thawing. This study evaluated the protective effect of Vitamin E supplementation as antioxidant on post-thaw DNA integrity of beluga (Huso huso) sperm. Collected sperm was divided into three parts and using DMSO alone as control group or in combination with vitamin E supplementation in 600 and 1200 μM has been diluted and was maintained for 30 days in liquid nitrogen storage tank. We used the Comet assay, a microelectrophoretic method to detect DNA damage in response to cryopreservation process in beluga spermatozoa. This technique was used to assess DNA integrity in the cells by measuring damages reflected as strand break. According to the results DNA damage rate in control freeze-thawed sperm was significantly higher than levels measured in fresh sperm. Adding the vitamin E had a dose dependent effect on the amount of DNA damage, as in 600 μM concentration, non-significant reduction in the level of damage than the control group was observed, but in 1200 μM, probably due to the pro-oxidant properties of the high-dose antioxidants significant increase in damage was assessed. In addition, the results indicated that the extent of damage to sperm motility caused by freeze-thawing was correlated with DNA breakage.