سمیت سلولی

The purpose of the present research is to investigate the possibility of producing nanofibers from chitosan extracted from black tiger shrimp (P. monodon) waste and the effect of nanofiber dimensions on the toxicity of primary polymers with the potential to be used in the production of wound dressings. In this research, two methods were used to produce nanofibers from the extracted chitosan polymer, which included single-needle and co-electrospinning, and in the second method, an attempt was made to produce fibers with core loading capability. Electrospun nanofibers were examined by field electron microscopy (FE-SEM) in terms of morphological characteristics. Also, various analytical technologies including FTIR (Fourier Infrared Spectroscopy) and XRD (X-ray Diffraction) were used to examine the structure of the produced nanofibers. Also, the toxicity of the produced nanofibers compared to the primary polymer was measured by the MTT method. The Fourier spectroscopy diagrams and X-ray diffraction peaks showed the presence of chitosan and polyvinyl alcohol polymers and their proper interactions. Also, the results showed that high-quality and continuous nanofibers without bead were obtained in both single-needle and core-shell methods. The interesting point is the creation of core space in core-shell nanofibers with the possibility of loading various polymers and secondary nanoparticles. This is a potential for its use in various applications, especially such as wound dressings. In addition to that, achieving less toxicity in the produced nanofibers compared to the primary polymers, proposed the potential of nanotechnology in different sciences.

Skin aging is accompanied by up regulation of the matrix metalloproteinase (MMP) expression and down regulation of collagen synthesis. Extracted sterols of marine algaes have been reported as anti-cancer agents and antioxidants, which can indicate the potential for anti-wrinkle properties in the human skin. In this study, the sterols extracted from Persian Gulf red algae Gracilaria salicornia were evaluated at concentrations 1.7, 3.5, 7, 14, 28, 56 and 112 μg / ml for determination of procollagen synthesis in human skin cells. To determine the acceptable dose of this precursor, the cytotoxicity test of G. salicornia sterols on the human skin fibroblast cells was tested at levels 2.3, 4.6, 9.3, 18.75, 37.5, 75, 150 and 300 μg/ml. The results indicate increased procollagen synthesis in the skin cells by increasing the concentration of sterols, and the lack of toxicity of this substance in the required dosage of the treatment. The extracts IC50 for human skin fibroblasts was 111 μg / ml. The effect of extracts on increasing of skin procollagen showed significant differences at concentrations 1.75 to 112 μg / ml (P<0.0001). Therefore, extracted sterols can play an important role on reducing the skin aging process due to the synthesis of skin cells procollagen. More research is needed for other uses of algae sterols for the use of cosmetic products.