جستجوی مقالات مرتبط با کلیدواژه "ویتلوژنین" در نشریات گروه "شیلات"

In this trial, changes of metabolically indices such as cortisol, glucose, vitellogenin in blood, testosterone, progesterone, 17-β estradiol in females and 11-ketotestosteron and cortisol in males carp (Cyprinus carpio) in Bandar Turkmen (South of the Caspian Sea) were investigated. The results (independent t-test) indicated that the amount of cortisol and progesterone in wild females were significantly higher than wild cultivated females (P<0.05). Also, the level of vitellogenin was significantly higher in wild females than wild cultivated females (P<0.05). However, the levels of 17-β estradiol, testosterone, glucose and calcium had no significant difference between wild and wild cultivated females (P>0.05). Also, the results of this study showed that the level of 11-ketotestosteron in wild male was significantly higher than wild cultivated male (P<0.05), and the level of cortisol in wild cultivated male was significantly higher than wild male (P<0.05).

The vitellogenin gene expression can be altered by some estrogenic plant compounds like Genistein and β-sitosterol. Therefore, the measurement of vitellogenin gene expression can be used as an indicator to determine their effect on reproductive performance of aquatic animals. In order to evaluate the effects of genistein and β-sitosterol on the expression of vitellogenin gene in the liver and ovary of Rutilus frisii Kutum, the fish were separately exposed to 3 levels of genistein and beta-sitosterol (500, 50 and 10 ng/L). After 21 days, the RNA extracted and expression of vitellogenin gene in both the liver and ovary was investigated by Real-time PCR. The results showed the level of vitellogenin gene expression in fish exposed to genistein was higher in liver than control and β-sitosterol treatment. This difference was not observed in the ovarian tissue. Because the main site of vitellogenin synthesis expressed liver and it was controlled by endogenous estrogen (E2), so, it seems phytoesterogenic compound such as genistein has been able to increase the relative expression of this gene in fish Exposed to 500 ng/L.

gold fish (Carassius auratus gibelio) is from the family of cyprinidae wich like common carp (cyprinus carpio). While there are many examples of the use of estrogens to skew sex ratios in fish few address teleost species and none have been published for the gold fish, Carassius auratus gibelio. vitellogenin is a big glicolipophosphoprotein that synthesis from liver under 17-b estradiol hormone.fingerlings of gold fish feeding by 17b estradiol to 6 mounths in 3 dose (10 25 50) mg per kg plus control treat. estradiol resolve in etanol then spray on food. in 25 and 50 mg per kg E2 all female fish product. with high in dose of E2 metabolism and growth of fish reduce.

Vitellogenin (Vtg) is a glycophospholipoprotein precursor of egg yolk which circulating levels of this protein in male and juvenile fish provide a sensitive indication of exposure to endocrine disruptor chemicals. The objectives of the present study were identification and purification of beluga (Huso huso) Vtg using Vtg selective precipitation (EDTA-Mg+2) and excision of Vtg band from reducing gel preparative. In this study, required plasma samples were collected from vitellogenic beluga and Vtg was identified using the specific characteristics of this protein (range of molecular weight, specificity for female, estrogen inducibility, precipitation in the presence of EDTA as well as specific reactivity of putative beluga Vtg with antibodies to beluga Vtg). Vtg highly purified by combination of Vtg selective precipitation and gel preparative. With SDS-PAGE under reducing conditions, beluga Vtg appeared as a major band of ~200 KDa. These data suggest that vitellogenin selective precipitation followed by gel preparative is an efficient procedure for the characterization and purification of beluga Vtg.

Annually، the large volumes of paper mill effluent to the environment in Iran. The major pulp and paper mill contain a large amount of alcohols and fatty acids and resin acids. Such materials stimulate vitellogenin production and suggested to be an estrogen mimic which cause sterility، sex reversal، yolk advance of replication and disruption of hormone cycles in fishes. Recently، vitellogenin gene quantification is considered as an efficient biomarker for the detection of estrogenic wastewater. Therefore، in this study the real time quantification of vitellogenin gene was used to evaluate the impact of pulp and paper mill on rainbow trout. After LC50 detection، the fish was exposed to 200 and 500 ppm (0. 1 and 0. 25 LC50، respectively) of the pulp and paper mill obtained from Mazandaran and Pars ahvaz company- Iran for 6 and 14 days and compared with control. To evaluate vitellogenin gene expression، the liver was taken from fish on days 0، 6 and 14. RNA was extracted from the tissue and evaluated on 1% agarose gel and Nanodrap. After cDNA synthesis، the samples were subjected to Real time PCR machine and gene expression analysis was performed using Sybr Green method. The results of this study indicated that the 500 ppm treatment stimulate vitellogenin gene expression at both 6th and 14th days exposure، when comparing with control (P< 0. 05). After 6 day exposure، the fish treated with 200 ppm pulp and paper mill did not show significant difference in respect to vitellogenin gene expression، where comparing with control while significant increase (P< 0. 05) was observed after 14th day. This suggest pulp and paper mill influent on vitellogenin gene expression and can be used as a biomarker for such wastewater.

The present study demonstrated the effects of nonylphenol (xenoestrogen) on Persian sturgeon (Acipenser persicus) vitellogenin (VTG) protein changes and production. Identification, purification and changes of vitellogenin protein in sera of fish treated by 17-beta estradiol and different concentration of nonylphenol, were performed. Gel filtration of this protein demonstrated the actual size of vitelogenin is 420KD in the native form, but vitellogenin in denaturated polyacrylamide gel had the form of dimmer protein. Purified cleavage vitelloginin (lipovitelin), which obtained from protein of Persian sturgeon oocyte, injected to rabbit for polyclonal antibody production and polyclonal antibody tested with treated sera for vitellogenin production as a biomarker of xenoestrogen using Wester blotting and ELISA method. All groups treated with 10 and 100mg/kg concentration of nonylphenol had significant effect on VTG protein production (p< 0. 05) but the group received the lowest dose of nonylphenol (1mg/kg) showed no significant change in VTG protein production. These results further support the negative effect of xenoestrogen on Persian sturgeon, indicating that this index can be used as a biomarker of xenoestrogen in aquatic ecosystems.