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  • مونا ظلی بوری آبادی، سعید گرگین*، یاسوزومی فوجی موری، پرویز زارع، علی صدوق نیری، ادی سوسانتو

    گربه ماهی (P. dussumieri) یکی از فراوان ترین گونه های تجاری در طول نوار ساحلی دریای عمان بوده است. در این مطالعه با هدف بررسی برخی از پارامترهای زیستی  گربه ماهی با استفاده از تور گوشگیر و تور پرساین در آب های ساحلی سیستان و بلوچستان انجام شده است. عملیات نمونه برداری از دی تا اسفند 1399 با استفاده از تور گوشگیر و تور پرساین انجام شد. تعداد 818 عدد گربه ماهی با تور گوشگیر و 300 گربه ماهی با تورپرساین در این نمونه برداری جمع آوری شد. و محدوه طول چنگالی صید P. dussumieri در تور گوشگیر و پرساین به ترتیب، 60/62-10/22 و 10/37-30/17 سانتی متر بدست آمد. طول بی نهایت (L∞) در این مطالعه 41/64 سانتی متر برآورد شد و طول بهینه صید آن 41/34 سانتی متر به دست آمد و طول بلوغ جنسی (Lm50) گربه ماهی 5/32 سانتی متر محاسبه شد. نتایج نشان داد که 10/38 درصد ماهیان صید شده نابالغ بوده اند و فرصتی برای تخم ریزی نداشته اند. اکثر ماهیانی که بوسیله تور پرساین صید شدند زیر اندازه بلوغ جنسی (Lm50) بودند.  به دست آمدن چنین پارامترهایی با مطالعات و برنامه ریزی های بلند مدت این ذخایر و اطلاعات مربوط به فروانی طولی، طول بی نهایت، طول بهینه صید و طول بلوغ جنسی (Lm50) می تواند تاثیر بالایی بر شناخت ذخایر و کاهش فشار صیادی بر ذخایر گربه ماهی در استان سیستان و بلوچستان باشد.

    کلید واژگان: گربه ماهی, P. Dussumieri, طول بهینه صید, تور گوشگیر, تور پر ساین}
    Mona Zellibooriabadi, Saeid Gorgin*, Yasuzumi Fujimori, Parviz Zare, Ali Sadough Niri, Adi Susanto

    Blacktip Sea Catfish (Plicofollis dussumieri) is one of the most abundant commercial species along the coast of the Oman Sea. In this study, with the aim of an investigation some biological parameters of Blacktip Sea Catfish using gillnet and purse seine in coastal waters of Sistan and Baluchistan province (Oman Sea). Sampling was conducted from January to March 2020 and 818 P. dussumieri were collected using gillnet and 300 P. dussumieri were collected using purse seine and the fork length range of P. dussumieri was 22.10-62.10 cm and 17.30-10.37 cm in gillnet and purse seine, respectively. The Asymptotic length (L∞) was estimated at 64.41 cm and the optimal catch length was 34.41 cm. The length maturity (Lm50) of P. dussumieri was calculated to be 32.5 cm. The results show 38.10% of the fish caught were immature and did not have a chance to spawn. Most of the P. dussumieri that were caught by purse seine were smaller than length at maturity (Lm50). Obtaining such parameters with studies and long-term planning of these stocks and the information related to the length frequency, Asymptotic length, optimum catchable length and length at maturity can have a great impact on the knowledge of the stocks and fishing pressure on aquatic stocks such as P. dussumieri in Sistan and Baluchistan province.

    Keywords: Blacktip Sea Catfish, Plicofollis Dussumieri, Optimum Catchable Length, Gillnet, Purse Seine}
  • پریا اکبری*، یوسف نگهداری جعفر بیگی

    با گسترش سریع صنعت آبزی پروری، به منظور تامین نیازهای پروتئینی انسان  استرس وحساسیت ماهی ها به بیماری در سیستم پرورش متراکم افزایش یافته است لذا استفاده از بسیاری از مکمل های غذایی نظیر پروبیوتیک ها، محرک های ایمنی و محصولات گیاهی برای مقابله با عوارض جانبی مرتبط با شرایط پرورش توسعه یافته است. تحقیق حاضر به منظور بررسی اثر سطوح مکمل گیاهی بیوهربال (پودر زنجبیل و رازیانه) فعالیت آنزیم های گوارشی (آمیلاز، لیپاز و پروتئاز) و کبدی (آسپارتات آمینوترانسفراز، آلانین آمینوترانسفراز و آلکالین فسفاتاز) و شاخص های بیوشیمیایی (گلوکز، پروتئین تام، گلوبولین، آلبومین، کلسترول و تری گلیسیرید) ماهی کفال خاکستری به مدت 60 روز صورت گرفت. در این مطالعه، تعداد 450 قطعه لارو کفال ماهی با میانگین وزنی 01/0±72/0 گرم0 در یک طرح کاملا تصادفی با 5 تیمار آزمایشی و 3 تکرار (با تعداد 30 قطعه در هر تکرار) که شامل تیمار آزمایشی شاهد (بدون استفاده از بیوهربال) و در تیمارهای آزمایشی 2، 3، 4 و 5 میزان استفاده از این مکمل گیاهی به ترتیب  5، 10، 15 و 20 گرم مکمل گیاهی بر کیلوگرم غذا بود مورد استفاده قرار گرفتند. در پایان آزمایش، نتایج حاصله نشان داد که بالاترین فعالیت آنزیم های پروتئاز (08/12±341 واحد بر میلی گرم پروتئین) آمیلاز (23/11±67/404 واحد بر میلی گرم پروتئین) و لیپاز (18/0±76/5 واحد بر میلی گرم پروتئین)، پایین ترین فعالیت آنزیم های آسپارتات آمینوترانسفراز (05/13±66/92 واحد بر میلی لیتر)، آلانین آمینوترانسفراز (15/1±33/15 واحد بر میلی لیتر) و آلکالین فسفاتاز (51/2±33/66 واحد بر میلی لیتردر تیمار حاوی g/kg 15مکمل گیاهی مشاهده شد.که با تیمار شاهد دارای تفاوت معنی دار بود (05/0>P). کمترین میزان کلسترول (78/3±66/120 میلی گرم بر دسی لیتر)، گلوکز (57/1±33/80 میلی گرم بر دسی لیتر) و تری گلیسیرید (01/9±06/120 میلی گرم بر دسی لیتر) و بیشترین میزان گلوبولین (15/0±52/1 گرم بر دسی لیتر) در تیمار 4 گزارش شد. در مجموع بر اساس نتایج این تحقیق، افزودن 20 گرم 20 مکمل گیاهی (زنجبیل و رازیانه) بر کیلوگرم به جیره غذایی ماهی کفال خاکستری به منظور بهبود فعالیت آنزیم های شاخص عملکرد گوارش و کبد و شاخص های بیوشیمیایی در این ماهی پیشنهاد می شود.

    کلید واژگان: کفال ماهی, بیوهربال, شاخص های بیوشیمیایی, آنزیم گوارشی, آنزیم های شاخص عملکرد کبد}
    Paria Akbary*, Yousef Negahdari Jafar Beigi

    With the rapid expansion of the aquaculture industry, in order to meet the protein needs of humans, stress and susceptibility of fish to disease in the intensive system has increased. Therefore, the use of many dietary supplements such as feathers probotics, safety stimulants, and plant products have been developed to combat the side effects associated with growing conditions. This experiment was conducted to evaluate the effect of of bioherbal feed supplement on the liver (aspartat amimotrasferase (ASP), alaninaminotransferase (ALT) and alkanin phosphatase (ALP)) and digestive enzymes (amylase, protease and lipase) and chemical parametese (glucose (GLU), cholesterol (CHO), triglyceride (TRI), total protein (TP), albumin (ALB) and globulin (GLO) of Mugil cephalus for 60 days. The experiment was conducted in a completely randomized design with 450 of grey mullet larvae (with average weight of 0.72±0.01g) in 5 treatments and 3 replicates (n=30 in each replicate) and included: control group without using bioherbal, an another groups (treatment 2, 3,4 and 5) the amounts of this herbal supplement were 5,10,15 and 20 g/kg food. At the end of experiment , the results showed that the highest the activity of protease (341±12.08U/mg protein), amylase (404.67±11.23 U/mg/protein) and lipase (5.76±0.18 U/mg protein), the lowest ASP (92.66±13.05U/mL), ALT (15.33±1.15 U/mL) and ALP (66.33±2.51 U/mL) enzymes were observed in the diet containing 20 g /kg bioherbal supplement in all of these parameters, treatment 5 (20 g/kg) (4) showed a significant difference compared with control treatment (P<0.05). Also, the lowest serum CHO (120.66± 3.78 mg/dL), GLU (80.33± 1.57 mg/dL) and TRI (120.06±9.01 mg/dL), the highest GLO (1.52±0.15 g/dL) were recorded in treatment 4. Finally, the present results suggest that diet containing 20 g/kg bioherbal supplement (ginger and fennel powder) could improve digestion and liver function index enzymes and biochemical parameters of Mugil cephalus larvae.

    Keywords: Mugil Cephalus, Bioherbal, Biochemical Parameters, Digestive Enzyme, Liver Fuction Index Enzymes}
  • سمیرا جعفریان، حسین آدینه، محمد فرهنگی*، محمد هرسیج، ضیاء کردجزی

    حمل و نقل و صید ماهی یک فعالیت رایج در آبزی پروری است که باعث استرس و تضعیف سیستم ایمنی می شود. هدف از این مطالعه بررسی اثرات استفاده از پری بیوتیک سانیار (پودر و مایع) در محیط بیوفلوک بر فعالیت های متابولیکی، شاخص های استرس و خون شناسی ماهی کپور معمولی تحت استرس صید و حمل و نقل بود. جیره های حاوی پری بیوتیک (1/0 و 2/0 گرم FP1 و FP2) و (1 و 2 میلی لیتر FL1 و FL2) و تیمار بدن افزودنی (FC) در محیط بیوفلوک و تیمار شاهد بدون افزودنی با آب تمیز (C) به ماهی کپور معمولی به مدت 60 روز داده شد. پایان دوره پرورش برای شبیه سازی استرس صید، ماهیان با تور ماهیگیری دستی در مخازن به مدت 10 دقیقه دنبال شدند. برای استرس ناشی از حمل و نقل طولانی مدت با تراکم ذخیره سازی 1 کیلوگرم (6 تیمار و هر یک با 3 تکرار) از کیسه های پلاستیکی (20 لیتر آب و 20 لیتر اکسیژن) به مدت 12 ساعت استفاده شد. بعد از اعمال استرس از ماهیان در دو گروه (صید و حمل و نقل) به طور مجزا خونگیری انجام شد. فعالیت های متابولیکی (آنزیم های کبدی، اوره و کراتینین)، گلبول های قرمز، هموگلوبین و هماتوکریت در تیمارهای پری بوتیکی تحت استرس کاهش معنی داری داشت. غلظت کورتیزول در تیمار FC (صید) و در تیمار شاهد (حمل و نقل) در مقایسه با دیگر تیمارهای آزمایشی افزایش معنی دار داشت. بطورکلی نتایج نشان داد که استفاده از پری بیوتیک تجاری سانیار در محیط بیوفلاک باعث کاهش اثرات استرسی صید و حمل و نقل برای ماهی کپور معمولی می شود.

    کلید واژگان: پری بیوتیک, بیوفلاک, ماهی کپور معمولی, استرس صید و حمل و نقل}
    Samira Jafaryan, Hossein Adineh, Mohammad Farhangi*, Mohammad Harsij, Zia Kordjazi

    Fish transportation and catching is a common activity in aquaculture that induces stress and immunosuppression. The aim of this study was to investigate the effects of using Sanyar prebiotic (powder and liquid) in biofloc environment on metabolic activity, stress indices, and hematological of common carp (Cyprinus carpio) under catching and transporting stress. Diets containing prebiotic (0.1 and 0.2 g powder; FP1 and FP2) and (1 and 2 ml liquid, FL1 and FL2) and control group without additive (FC) in biofloc system and the control group without additive with clean water (C) were fed to common carp for 60 days. At the end of the rearing period, to simulate catching stress, the fish were followed in the tanks with hand fishing nets for 10 minutes. For stress caused by long transportation with a stocking density of 1 kg (6 treatments and each with 3 replications), plastic bags (20 liters of water and 20 liters of oxygen) were used for 12 hours. After applying stress, blood sampling was done separately from the fish in two groups (catching and transportation). Metabolic activities (hepatic enzymes, urea, and creatinine), Red blood cells, hemoglobin, and hematocrit had a significant decrease in prebiotic treatments under stress. Cortisol concentration in the FC treatment (catch) and in the control treatment (transportation) significantly increased compared to other experimental treatments. In general, the results showed that using Sanyar commercial prebiotics in the biofloc environment reduces the stressful effects of catching and transportation for common carp.

    Keywords: Prebiotics, Biofloc, Common Carp, Fishing Stress, Transportation}
  • ضیاء کردجزی*، محمد فرهنگی، محمد لاریجانی

    وضعیت ذخیره بسیاری از منابع شیلاتی در جهان به دلیل دسترسی محدود به داده برای ارزیابی ذخایر آنها ناشناخته است. برای حل این چالش چندین مدل ارزیابی ذخایر، مانند مدل تولید مازاد شفر و فاکس معرفی شدند که برای برآورد وضعیت ذخیره آبزیان به داده های محدودی مانند داده های صید و صید به ازای واحد تلاش صیادی (CPUE) نیاز دارند. در این مطالعه از داده های صید و صید به ازای واحد تلاش صیادی (CPUE) ماهی کپور دریایی درطول سالهای 1378 تا 1401 جنوب شرقی دریای کاسپین برای برآورد حداکثر تولید پایدار (MSY) از طریق مدل تولید مازاد استفاده شد. با استفاده از مدل catch-MSY، سه پارامتر مورد نیاز برای برآورد MSY، شامل نرخ رشد جمعیت (r) در سال (که ترکیبی از رشد وزنی افراد، ریکروتمنت و مرگ و میر طبیعی است)، ظرفیت حمل بیولوژیکی جمعیت (K) (که معادل با بیوماس یک جمعیت بهره برداری نشده است)، و بیوماس آغازین در یک جمعیت در حال بهره برداری (Binit) به ترتیب 075/0 در سال، 6952 تن و 3500 تن برآورد شد. MSY برآورد شده با این پارامترها در مدل SPM برابر با 130 تن بود. در حالی که نتایج برازش داده ها با استفاده از نقاط آغازین چندگانه نشان داد MSY حدود 1320 تن است. نمودار فازی نشان داد زیست توده فعلی از زیست توده (مرجع) حد مجاز و زیست توده مرجع هدف پایین تر است؛ که می توان بیان کرد ذخیره ماهی کپور در جنوب شرقی دریای کاسپین ناپایدار است و به مرحله  صید بی رویه (overfished) رسیده است. از آنجاییکه، بقاء ماهی کپور به بازسازی ذخیره آن از طریق رهاسازی بچه ماهیان بستگی دارد، باید توجه ویژه ای به مدیریت صید این گونه از طریق جلوگیری از صید افراد زیر اندازه استاندارد صید و صید غیرمجاز صورت گیرد.

    کلید واژگان: مدل تولید مازاد, حداکثر تولید پایدار, صید بیرویه, Cyprinus Carpio, دریای خزر}
    Ziya Kordjazi*, Mohammad Farhangi, Mohammad Larijani

    The stock status of most fisheries resources is not well-known, which is associated with limited data available for stock assessment. To overcome this gap, several data-limited stock assessment models, including the surplus production model, were applied to estimate stock status of these data-limited resources where no sufficient data is available to apply the conventional stock assessment models. To estimate MSY, catch and CPUE data for Cyprinus carpio caught from the southeastern Caspian Sea were applied. Twenty-two years data (1999-2022) were analysed by SPM to estimate MSY. Three parameters including population growth rate (r), population carrying capacity (K), and initial biomass for an exploited population (Binit) required for estimating MSY were guessed 0.075 (/yr), 6952 tones, and 3500 tones, respectively by the catch-MSY model. The estimated MSY using these guessed parameters by SPM was 130 tones. While, estimated MSY using the repeated (multiple) starting points was 1320 tones. Phase plot indicated that the current status was both below the limited and the target biomass reference points. These illustrated the stock of C. carpio in the southeatern Caspian Sea is unsustainable and overfished. Since, survival of C. carpio depends the releasing hatchery reared juveniles to the wild, specific fisheries management should be applied to prevent the undersize catch and illegal fishing.

    Keywords: Surplus Production Model, MSY, Overfishing, Cyprinus Carpio, Caspian Sea}
  • هادی پورباقر*، سهیل ایگدری، حامد شعبانلو

    ماهیان آب شیرین اهمیت زیادی در بوم سازگان های آب شیرین داشته و کارکردهای مختلفی در بوم سازگان ایفا می کنند. اندازه گیری تنوع زیستی این ماهیان در دستور کار بسیاری از مطالعات بوم شناختی قرار دارد. اندازه گیری تنوع زیستی به نوع داده و هدف مطالعه وابسته و تاکنون نیز روش های مختلفی برای محاسبه آن تدوین شده است. مطالعه حاضر در نظر داشت تا با آنالیز فرکتال تنوع زیستی ماهیان حوضه دریاچه ارومیه را با استفاده از داده های حضور ماهی محاسبه نماید. افزون بر این مقدار عدم قطعیت بعد فرکتال محاسبه شده با شبیه سازی بدست آمد. این عدم قطعیت، دامنه تغییرات بعد فرکتال را مشخص نمود. نتایج نشان داد که بعد فرکتال ماهیان آب شیرین حوضه دریاچه ارومیه در حد متوسطی قرار دارد (774/1). با توجه به خشک شدن دریاچه ارومیه این نتایج نشان می دهد که تنوع زیستی این ماهیان در حد حساسی قرار داشته و ممکن است به سمتی منفی گرایش پیدا نماید. حد متوسط تنوع زیستی ماهیان حوضه همچنین می تواند حاکی از حد متوسطی از ارتباط بین جمعیت ها و جریان ژنی باشد. روش بکار رفته برای مطالعه تنوع زیستی و تغییرات آن می تواند برای سایر بوم سازگان های آبی و حوضه های آبخیز مورد استفاده قرار گیرد. این یافته ها اهمیت تلاش های حفاظتی و تحقیقات بیشتر را در کاهش تاثیرات منفی محتمل بر جمعیت های ماهیان آب های شیرین و اکوسیستم آنها برجسته می کند.

    کلید واژگان: بومی گرایی, تنوع زیستی, دریاچه ارومیه, فرکتال, ماهیان آب شیرین}
    Hadi Poorbagher*, Soheil Eagderi, Hamed Shabanloo

    Freshwater fishes have great importance in freshwater ecosystems and play various roles in ecosystems. Measuring the biodiversity of these fish is a key focus in many ecological studies. The measurement of biodiversity depends on the type of data and the study objectives and various methods have been developed to calculate it. This study aimed to calculate the biodiversity of fish in the Lake Urmia basin using fractal analysis based on fish presence data. Additionally, the calculated fractal dimension was obtained with simulation, which determined the range of fractal dimension variations. The results indicated that the fractal dimension of freshwater fish in the Lake Urmia basin is at an average level. Considering the drying of Lake Urmia, these results suggest that the biodiversity of these fish is highly sensitive and may tend towards negative changes. The average level of biodiversity among fish in the basin can also indicate an average level of population connectivity and gene flow. The methodology used to study biodiversity and its changes can be applied to other aquatic ecosystems and watersheds. These findings highlight the importance of conservation efforts and further research to mitigate potential negative impacts on freshwater fish populations and their ecosystems.

    Keywords: Biodiversity, Endemism, Fractal, Freshwater Fish, Lake Urmia}
  • سامیه کتوکی، حجت الله جعفریان، حسنا قلی پور، پونه ابراهیمی*

    در مطالعه حاضر تاثیر تراکم های ذخیره سازی در چهار سطح مختلف (30، 45، 60 و 75 عدد لارو در هر مترمکعب) بر پارامترهای رشد و شاخص های بیوشیمیایی سرم خون در لاروهای ماهی قزل آلای رنگین کمان (Oncorhynchus mykiss) با میانگین وزنی 08/0±235/0 گرم (انحراف معیار±میانگین) در 12 مخزن فایبرگلاس با ظرفیت آبگیری 10 لیتر در یک دوره پرورش 45 روزه بررسی گردید. در پایان دوره آزمایش اختلاف معنی داری در پارامترهای رشد و کارایی تغذیه بین تیمارهای مختلف آزمایشی مشاهده نشد (05/0p>). درحالی که نتایج حاصل از آنالیز پارامترهای بیوشیمیایی سرم خون دارای اختلافات معنی داری بین تیمارهای آزمایشی بودند (05/0p<). بر این اساس، بالاترین میزان پروتئین کل (10/0±40/7)، آلانین آمینوترانسفراز (00/1±00/23)، آلکالین فسفاتاز (18/8±00/243)، آمیلاز (52/1±66/22)، لیپاز (55/6±00/111)، کلسیم (05/0±66/15) و کمترین میزان کورتیزول (73/1±00/36) و آلبومین (11/0±76/10) در تیمار با بیشترین میزان تراکم ذخیره سازی (75 عدد لارو در هر مترمکعب) بود (05/0p<). بالاترین میزان گلوکز سرم (56/5±00/139) در تیمار با تراکم 60 عدد لارو و بالاترین میزان فعالیت آنزیم آسپاراتات آمینوترانسفراز در دو تیمار با تراکم های ذخیره سازی 30 و 45 عدد لارو در هر مترمکعب (U/dl 57/0±33/7) ثبت گردید (05/0p<). درمجموع، بر اساس نتایج به دست آمده افزایش تراکم لاروهای ماهی قزل آلای رنگین کمان هیچ گونه تاثیر معنی داری بر بازده پرورش این گونه ندارد؛ اما این افزایش تراکم ازنظر استرس زایی و تاثیر بر فاکتورهای ایمنی کاملا معنی دار بود.

    کلید واژگان: لارو قزل آلای رنگین کمان, تراکم ذخیره سازی, فاکتورهای بیوشیمیایی سرم, ایمنی}
    Samieh Katooky, Hojatolah Jafaryan, Hosna Gholipor, Pone Ebrahimi*

    A experiment was carried out to evaluate the effect of four levels of stocking density (30, 45, 60 and 75 fish/m3) on growth parameters and biochemical factors of blood serum of Oncorhynchus mykiss larvae with average mean weight 0.235±0.08 g were stocked in 12 fiberglass tank (10 L) was studied for a period of 45 days. At the end of the experiment, were observed no significant differences between growth parameters and feed efficiency among treatments (p>0.05). However, Analysis of biochemical factors of serum blood showed significant difference between treatments (p<0.05). The highest amount of blood serum of total protein (7.40±0.10), ALT (23.00±1.00) and ALP (243.00±8.18) activity enzymes, amylase (23.66±1.52), lipase (111.00±6.55) and calcium (15.66±0.05), as well as the lowest amount of cortisol (36.00±1.73) and albumin (10.76±0.11) was observed in treatment containing maximum stocking density level at 75 fish larva per m3 (p<0.05). Also, the highest amount of glucose (139.00±5.56) was observed in treatment at 60 fish larva per m3 and the highest AST activity enzyme (7.33±0.57) was observed in two treatment at 30 and 45 fish larva per m3 (p<0.05). Finally, according to the results, an increase in stocking density of O. mykiss larvae cannot be affected the efficiency of fish farming. But, this increasing density had significant effect on immune factors and stressors.

    Keywords: Rainbow Trout Larvae, Stocking Density, Growth, Biochemical Factors, Immunity}
  • سید مرتضی حسینی، سید حسین حسینی فر*، ملیکا قلیچ پور، عباسعلی اقایی مقدم، بهروز قره وی، سید محمود عقیلی، عیسی شریف پور، محمود حافظیه، محمدرضا فایضی

    این تحقیق به منظور بررسی اثر افزودن نمک و باکتریBacillus subtilis (IS02)   به جیره غذایی بر بقاء، پاسخ کورتیزول و شاخص های آنتی اکسیدانی بچه ماهی نورس کپور دریایی، Cyprinus carpio، در مواجهه با تنش شوری انجام شد. بچه ماهی کپور (میانگین 1/0 ± 1/1گرم) به مدت 15 روز با جیره های غذایی حاوی 5 و 10 درصد نمک یا 108 × 5/2 و 109 × 5/2 کلنی گرم (cfu/g) پروبیوتیک تغذیه و سپس به طور مستقیم به آب لب شور با غلظت 13 گرم بر لیتر منتقل و پس از 3 و 10 روز نمونه گیری شدند. بقاء در همه تیمارها بالای 96 درصد بود. میزان کورتیزول پایه و پس از تنش شوری در تیمارهای پروبیوتیکی به طور معنی داری کمتر از سایر تیمارها بود. فعالیت سوپراکسید دیسموتاز در تیمارهای پروبیوتیکی بالاتر از سایر تیمارها بود. جیره های غذایی اثر معنی داری بر فعالیت کاتالاز نداشتند. بر اساس نتایج این تحقیق، استفاده از B. subtilis در جیره غذایی می تواند باعث کاهش استرس و افزایش ظرفیت آنتی اکسیدانی در بچه ماهی کپور نورس شود.

    کلید واژگان: استرس, بازسازی ذخایر, شوری, جیره غذایی, پروبیوتیک, نمک}
    Seyyed Morteza Hoseini, Seysd Hosein Hoseinifar*, Melika Ghelichpour, Abbasali Aghaei Moghaddam, Behrouz Gharavi, Seyed Mahmoud Aghili, Isa Sharifpour, Mahmoud Hafezieh, Mohammad Reza Fayezi

    This research was conducted in order to investigate the effect of adding salt and Bacillus subtilis bacteria (IS02) to the diet on the survival, cortisol response and antioxidant indices of sea carp, Cyprinus carpio, under salinity stress. Baby carp fish (average 1.1 g) were fed with diets containing 5 and 10% salt or 108 x 5.2 and 109 x 5.2 colony grams (cfu/g) of probiotics for 15 days and then to They were directly transferred to saline water with a concentration of 13 grams per liter and samples were taken after 3 and 10 days. Survival in all treatments was above 96%. The amount of cortisol at baseline and after salinity stress in probiotic treatments was significantly lower than other treatments. Superoxide dismutase activity was higher in probiotic treatments than other treatments. Diets had no significant effect on catalase activity. Based on the results of this research, the use of B. subtilis in the diet can reduce stress and increase the antioxidant capacity in juvenile Norse carp.

    Keywords: Stress, Stock Rehabilitation, Salinity, Diet, Probiotics, Salt}
  • کیوان عباسی رنجبر، علیرضا میرزاجانی*، مهدی مرادی چافی، یعقوبعلی زحمتکش

    سه گونه ماهی سفید، سیاه کولی و شاه کولی در زمره ارزشمندترین ماهیانی هستند که به رودخانه های حوضه جنوبی دریای کاسپین از جمله رودخانه سفیدرود مهاجرت می کنند. در این مطالعه پراکنش زمانی و مکانی بچه ماهیان این گونه ها بر اساس حضور و فراوانی در 8 ایستگاه رودخانه سفیدرود از مصب تا سد سنگر طی یکسال از اسفند 1400 تا دی 1401 مورد بررسی قرار گرفت. برحسب شرایط محیطی، نمونه برداری با الکتروشوکر، تور پرتابی و پره چشمه 6 میلی متر انجام گرفت. نتایج بررسی نشان داد که فراوانی کلی بچه ماهیان در ماه های زمستان حداقل بوده و فراوانی حداکثری آنها با زمان بعد از تولید مثل مولدین آنها منطبق است. فراوانی حداکثری این بچه ماهیان در زمان های مختلف در کنار پراکنش مکانی، الگوی مهاجرتی آنها به سمت دریا را نیز نشان می دهد. فراوانی بچه ماهی سفید در بستر سنگلاخی و سنگریزه ای ایستگاه های بالادست در اردیبهشت ماه و فراوانی بچه ماهیان شاه کولی و سیاه کولی در بسترهای سنگریزه ای- گلی ایستگاه های میانی تا مصب در مرداد- شهریور بیشترین بودند. حضور بچه ماهی سفید در ناحیه مصبی و دهانه سفیدرود طی تابستان مشهود بوده و در مرداد ماه ماهیان با وزن متوسط 2 گرم در 8 کیلومتری مصب فراوانتر بودند، دو گونه دیگر تا آبان ماه در ایستگاه های میانی با فراوانی بالا حضور داشتند. حضور بچه ماهی سفید در اوزان بالاتر از 3 گرم در ایستگاه های مختلف در پائیز و زمستان، سازگاری و ماند گاری تعدادی از بچه ماهیان در بخش های مختلف رودخانه را نشان می دهد. نتایج بررسی حاضر، می تواند برخی رهنمودها را برای شیلات ایران مطرح نموده تا در زمانی پیشتر، لارو ماهی سفید را در مناطق بالادست، یا بچه ماهیان با وزن کمتر را در میان دست رودخانه، رهاسازی نمایند. این برنامه می تواند در قالب یک برنامه راهنما آزمون گردد. 

    کلید واژگان: گیلان, تنوع ماهیان, فراوانی, خصوصیات زیستی, رهاسازی بچه ماهی}
    Kyvan Abbasi Ranjbar, Alireza Mirzajani*, Mehdi Moradi Chafi, Yaghobali Zahmatkesh

    Three species Rutilus frisii, Alburnus chalcoides and Vimba persa are the most valuable fish that migrate to southern Caspian rivers especially Sefidroud river. In this study, the temporal and spatial distribution of was investigated based on presence and abundance of fingerling fish in 8 stations of the Sefidroud River, from the estuary to the Sangar Dam, during one year from March 2021 to December 2022.</span></span></span> According to the environmental conditions of stations, the sampling was done with an electrofishing instrument and a throwing net with mesh size 6 mm. The results showed that the abundance of fish fingerlings is minimal in the winter and their maximum abundance coincides with the reproduction time and after their adult spawning. The maximum abundance of these fingerlings along with their spatial distribution, also shows their migration pattern towards the Caspian Sea. The abundance of Rutilus frisii in the pebble and gravel beds of the upstream stations was the highest in April and the abundance of the Alburnus chalcoides and Vimba persa in the gravel-mud beds of the middle stations up to the estuary was the highest in August-September. The presence of Rutilus frisii fingerling, with an average weight of 2 g. was high in near to estuary in August while the other two species were present in the middle stations with high abundance until November. Rutilus frisii with weigh more than 3 grams in different stations in autumn and winter indicate the adaptability and residency of a number of fish in the river. The present study can provide some guidelines to the fish stocks restoration centers of Caspian Sea for releasing Kutum fry in the upstream areas, or fish fingerling in the middle of the river at an earlier time. This plan can be tested in a pilot program.

    Keywords: Giulan, Fish Diversity, Abundance, Biological Characteristics, Release Of Fish Fingerling}
  • S. Esmaeilpour-Poodeh*, H. Rahmani, R. Ghorbani, F. Abbasi

    Age, growth, and reproductive traits of the South Caspian Spirlin in the southern part of the Caspian Sea basin, Tajan, River, were investigated. A comprehensive study was conducted on 663 specimens including 226 females, 297 males, and 140 unsexed individuals with a total length ranging from 25.88 to 121.66 mm. The specimens were collected using an electrofisher at monthly intervals. The female-to-male sex ratio was calculated as 1: 1.29. The growth rings on the scale were employed for age determination in this study. Both sexes exhibited only four age groups (0+ to 3+), with the 2+ age group dominating the population. Females consistently displayed larger sizes compared to males. The von Bertalanffy growth function yielded L t =123.90 (1- e -0.25(t+0.87) )  and L t =128.10 1- e -0.29 t+0.69  for males and females, respectively. Furthermore, the Fulton condition factor value was significantly higher in females than males. Notably, the maximum gonadosomatic index for both males and females coincided with the spawning seasons (May to August). Absolute fecundity varied from 290 to 1710 oocytes, with a mean of 793 oocytes. This study presents comprehensive data on the biological characteristics of the South Caspian Spirlin, providing valuable insights for fish stock assessment studies.

    Keywords: Population Parameters, Fecundity, Age Determination, Gonadosomatic Index}
  • M. Bavarsad, A. Abed-Elmdoust, M.R. Tabandeh, H. Farahmand, M. Alishahi, A. Mirvaghefi, A. Avazeh, M. Adel*, A. Jafari, M.J. Zorriehzahra, T. Citarasu

    Cyprinid herpes virus 3 (CyHV-3) is a significant threat to the production of common carp and koi, resulting in high mortality rates and posing a risk to the long-term sustainability of carp aquaculture. The presence of this disease in Iran has been confirmed by the veterinary organization and reported to OIE, with evidence suggesting its presence since 2021 in Iranian Koi and carp farms. Given the economic importance and rapid spread of CyHV-3, this review aims to provide a comprehensive summary of the current knowledge and research on the virus and its disease in Iran and worldwide.  The fish farmers and authorities in the country need to take necessary measures to prevent the spread of the disease and mitigate its impact on the aquaculture industry.

    Keywords: Koi Herpes Virus, Cyhv-3, Koi, Common Carp, KHVD, Iran}
  • Z. Talebi*, E. Rahimi, M.H. Abolhasani

    Yersinia enterocolitica is the cause of yersiniosis in humans and its symptoms range from mild diarrhea to mesenteric lymphadenitis that may lead to appendicitis. In this study, the frequency and antibiotic resistance of Yersinia enterocolitica isolated from fish caught in Anzali wetland were investigated. Sampling was carried out for one year in four seasons from the autumn 2018 to the summer 2019 from four geographical directions: east, west, north and south, from different wetland levels. A total number of 91 fish were sampled from the body of these fish. Microbiological tests including biochemical tests, specific culture, and PCR were performed. In the specific culture method, 20 samples (21.97%) were contaminated with Yersinia enterocolitica. For the final confirmation of PCR, 20 samples were isolated from 14 samples (15.4%) containing specific culture method (6 Carp, 3 Anchovy, 0 Bream, 4 Pike Perch and 1 Pike) were infected with Yersinia enterocolitica. The chi-square test showed that there was no statistically significant difference between the various seasons (p >0.05), while there was a significant difference between fish species (p <0.05). Isolates from Yersinia enterocolitica using an antibiogram test of 10 antibiotics, the most susceptible isolates, Imipenem (92.8%), Kanamycin and Gentamicin (85.7%), Ceftazidime (78.57%). Cotrimoxazole and Nalidixic acid (71.4%) were the most resistant isolates to Ampicillin (64.28%), respectively. The results showed high levels of contamination of Yersinia enterocolitica, and indicative of potential dangers of Yersinia enterocolitica pathogenicity, especially in immunocompromised and susceptible individuals. Further studies on the pathogenicity of Yersinia enterocolitica in fish within the Anzali wetland and preventing sewage from entering the wetland are suggested.

    Keywords: Frequency, Fish, Yersinia Enterocolitica, Wetland, Antimicrobial Resistance}
  • N. Arshad*, F. Kiran, M. Kamran, K. Saboor, A. Azeem, M.B. Su'ud, M.M. Alam, H. Tariq

    Plastic pollution has emerged as a pervasive global concern, with plastic waste contributing to the generation of microplastic particles (MPs) that have now integrated seamlessly into the human food chain. Intriguingly, early life exposure to MPs in children may occur through breast milk. This preliminary involved the analysis of human breast milk samples collected from 15 healthy breastfeeding mothers of the fishing community (BMSF) with daily habits of sea-food consumption and 8 healthy breastfeeding mothers (BMSH) with no history of seafood consumption in the past month and analyzed using microscopy and micro-Fourier-transformed infrared spectroscopy. In the BMSF group, a total of 276 MPs while 49 MPs were found in the BMSH group, indicating a substantial difference in MPs presence. The most prevalent MPs in both groups were fibers and pellets which fell within the size range of 5 to 18 µm, with polyethylene, polyvinyl chloride, and polypropylene being the most frequently observed types. Statistical analysis revealed a significant difference in the abundance of MPs between the two communities (F=13.57, P=0.001; Mann-Whitney U test, p<0.05). These findings emphasize the urgent need for further research to explore the potential health implications of MPs exposure through breast milk, especially in economically disadvantaged and vulnerable communities’ i.e. pregnant women, lactating mothers and children, with high seafood consumption.

    Keywords: Microplastics, Breastmilk, Seafood Consumption, Breastfeeding Mothers, Karachi}
  • M. Salehi Balashahri, M.M. Dehshiri, J. Sohrabipour*, I. Mehregan

    Caulerpa racemosa is edible marine green algae of the Caulerpaceae family. C. racemosa is consumed as a sea vegetable in many countries, especially Southeast Asian countries. In recent years, molecular studies caused some changes in the taxonomic positions of the members of the genus Caulerpa. It is necessary to accurately identify species of this genus based on modern molecular studies due to the consumption of some species and also the toxic effect of some other species of this genus. Some species of Caulerpa have already been reported from the southern coasts of Iran based on preliminary morphological studies. The purpose of this study was to collect and accurately identify the species of the genus Caulerpa in the coastal waters of the Persian Gulf and the Gulf of Oman in the southern coastlines of Iran. This study was based on the combination of molecular and morphological characteristics studies of the collected samples of this genus in this area. In the current article, the results obtained about the Caulerpa racemosa are presented. Samples of the genus were collected from different coastal areas of the Persian Gulf and seashores of Oman Gulf along the geographical borders of Iran and transported to the laboratory in cool conditions. After cleaning and washing, some of the cleaned samples were dried in silica gel and used for DNA extraction and thereof PCR process based on tufA gene. In addition, herbarium samples required for morphological studies were prepared and deposited in the herbarium of Agriculture and Natural Resources Research and Education Center of Hormozgan province. The results of this study relatively solve the confusion in Caulerpa racemosa- peltata complexity for Iranian members of the complex. In addition to morphological features, the current molecular investigation also helped to accurately identification of the members of this complex in the Iranian seashores of the Persian Gulf and Gulf of Oman. As a highlighted conclusion, the current research on Caulerpa species from the Iranian seashores of the Persian Gulf and Gulf of Oman resulted in resolving two species including Caulerpa sertulariodes (S. G. Gmelin) Howe forma farlowii (Weber-Van Bosse) Børgesen, which was resolved as Caulerpa selago (Turner) C. Agardh (Salehi Balashahri et al., 2021) and Caulerpa racemosa var. macrophysa also resolved as Caulerpa racemosa (Forsskål) J. Agardh.

    Keywords: Seaweed, Caulerpceae, Gulf Of Oman, Persian Gulf, Tufa Gene, Green Algae}
  • S. Adibi, M. Ramezani*, S. Kakoolaki, R. Kazempoor

    It is well known that ammonia nitrogen is a major pollutant in aquaculture, which can have toxic effects on fish. When absorbed in toxic concentrations, ammonia nitrogen can enter the bloodstream of fish, affecting blood parameters, immune responses, and causing oxidative damage and neurotoxicity. Recently, a study was conducted to investigate the toxic effects of ammonia on the blood, liver, growth, tissue damage, and immune indices of Cyprinus rubrofuscus in the presence of formaldehyde bisulfite sodium (FBS). The study involved 360 C. rubrofuscus, which were randomly distributed among 24 aquaria with a ratio of FBS to ammonia set at 31mg/L:1mg/L. The experiment was conducted with 15 fish in 6 treatments and 4 repetitions until 50% mortality was observed. The growth, histology, hematology, immunity, liver enzymatic and biochemical features of the fish were studied, and the results were analyzed using one-way analysis of variance (One-Way ANOVA) and Duncan’s test. The study found that the blood, immune, and liver indices of koi fish changed in the presence of FBS. Additionally, adding FBS to aquarium water reduced nitrogen compounds in fish storage tanks, further reducing the nitrogen compounds in fish aquaria.

    Keywords: Ammonia, Antibody-Enzyme, Bioaccumulation, Liver Indices, Toxicity, FBS}
  • M.S. Hashemi*, F. Mohammadizadeh, A.H. Bahri, M. Hafezieh, R. Ghorbani Vagheei

    The  present  study  was  conducted  to  evaluate  the  dietary  effect  of  nucleotide  on  growth performance and intestinal microbial flora in Siberian sturgeon (Acipenser baerii). Four hundred fifty fish of A. baerii (39.77±10.24 g initial weight) were distributed into 15 tanks (300 L) and they were fed with different levels of nucleotide (Ascogen®) including 0.0 (control), 0.2 (diet 1), 0.4 (diet 2), 0.6 (diet 3), and 0.8 (diet 4) g kg -1 in triplicates for nine weeks. The fish were fed daily until apparent satiety. The results showed no significant differences in the final length and survival rate among the experimental groups (p>0.05). However, the final weight, weight gain, daily growth rate, specific growth rate, and condition factor were significantly enhanced in the groups fed with diet 3 and diet 4 (p<0.05). The count of intestinal lactic acid bacteria (LAB) was the same among the experimental groups (p>0.05), while the total count of the intestinal bacteria was significantly decreased compared to the control group (free from nucleotide) (p<0.05). In conclusion, most of the growth indices were improved in the fish fed with nucleotide at 0.6 and 0.8 g kg -1. However, the treated diets could not affect the intestinal LAB population in Siberian sturgeon.

    Keywords: Nucleotide, Siberian Sturgeon, Growth, Gut Bacterial Community}
  • M. Takafouyan, B. Mohammadian*, T. Mohammadian, M. Mesbah

    Aquaculture represents a pivotal economic sector worldwide, meeting the escalating food demands of the expanding global population. Consequently, this research aimed to assess the incidence of fatty liver in Asian sea bass (Lates calcarifer) subjected to a diet enriched with lactic acid bacteria and evaluate their survival against Streptococcus iniae infection. The present study examined 240 sea bass (109 ± 10.5 g average weight) that were randomly assigned into four treatments with three replicates (25 specimens per treatment) for 60 days. The treatments comprised the following: First treatment: fish were fed with commercial feed. Second treatment: fish were provided with feed containing 109 CFU/g of Lactobacillus plantarum bacteria. Third treatment: fish were fed with feed containing 109 CFU/g of Lactobacillus pentosus bacteria. Fourth treatment: fish were provided with feed having 109 CFU/g of L. pentosus bacteria combined with L. plantarum in equal proportions. At the end of the experiment, the growth performance, the survival rate against the pathogenic bacteria S. iniae and the amount of fatty liver were evaluated. The findings disclosed enhanced growth indicators in the second treatment (strain 140) during the initial 30 days. Furthermore, statistically significant disparities were noted in the third treatment (2P) concerning PER, SGR, WG, RGR, and DWG during the subsequent 30-day period (P<0.05). Liver pathology examination demonstrated that most treatments resulted in the development of fatty liver. However, the third treatment (L. pentosus) exhibited the lowest incidence of fatty liver when endogenous probiotics were incorporated into the diet. Post-challenge with S. iniae, the mortality rate in the probiotic treatments L. pentosus (P2) and L. plantarum (140) significantly surpassed that of the control group (P<0.05). The findings underscore the absence of synergistic interactions between the two experimental probiotics on the 60th day, as the combined group displayed diminished growth performance compared to the individual groups. Moreover, the use of L. plantarum and L. pentosus bacteria, particularly the latter, has been shown to significantly improve several growth indicators, as well as the food conversion ratio. Consequently, these probiotics are recommended as dietary supplements for Asian sea bass.

    Keywords: Indogenous Probiotic, Bacteria, Growth, Fatty Liver, Streptococcosis, Asian Sea Bass, Growth Performance, Fatty Liver Change Streptococcus Iniiae}
  • B. Taylan*

    European anchovy (Engraulis encrasicolus) is one of the most important commercial species in the Mediterranean Sea. 4310 studied records were reported from the Google Scholar database between 1980 and 2022. A search with the keywords "Engraulis encrasicolus" or "European anchovy" and "growth" and "sex" and "length" and "length-weight relationship" returned 201 results, of which only research articles were included in the compilation. A total of 20 studies were evaluated in the present study. In the studies on the growth and length-weight relationship of anchovy species, pooled random effect meta-analysis, subgroup analysis, and moderator analysis were carried out. It was found that sex had a significant effect on the mean and standard deviation of length of male and female individuals. The results showed that there was a positive correlation between male and female individuals. The moderator analysis was carried out to examine this high heterogeneity. Accordingly, L∞ and K values and b coefficients of male and female individuals in different regions of the Mediterranean Sea should not be considered equivalent. The results of the study are generalizable to the reviewed studies except for the b coefficients of males and females.

    Keywords: European Anchovy, Engraulis Encrasicolus, Meta-Analysis, Growth, Length-Weight Relationships, Mediterranean Sea}
  • P. Chitrakar, M.A. Khalid*, S.N. Labh

    Nepal has a diverse geography, ranging from an altitude of 60 m to the world's highest peaks at 8849 m. Fish diversity has been observed to extend up to an elevation of 3600 m. With its intricate topography, fluctuating climatic conditions, and abundant water resources, the region exclusively comprises freshwater habitats spanning 745,000 ha. These habitats include rivers, lakes, ponds, marshes, reservoirs, and irrigated rice fields. There are roughly 6,000 rivers and creeks in the country (194,471 km² drainage area). Four major river systems, namely Koshi, Gandaki, Karnali, and Mahakali, originate from the upper Himalayas at an altitude greater than 5000 m above sea level and drain about 75% of the country. Freshwater fishes are often overlooked in biodiversity conservation efforts, leading to a significant decrease in fish diversity, particularly in densely populated urban areas and wetlands in the southern plains of Nepal. The decrease in fish diversity is likely linked to inadequate prioritization, funding, and human resources allocated to fish conservation. According to the literature and FishBase database records, the freshwater river system in Nepal contains a total of 255 fish species from 12 orders, 41 families, and 124 genera. This includes 15 fish species that are unique to Nepal and 15 that are not native to the region. Additionally, 34 fish species in this system are considered threatened and have been listed under the IUCN Red List. These fish have pronounced vertical dispersion throughout the country and horizontal distribution within the area and continent, displaying diverse adaptations. This research specifically examines the fluctuations in water quality and the range of fish species, together with their conservation status in the rivers of Nepal.

    Keywords: Spatiotemporal, Variability, Fish Diversity, Lakes, Nepal, River, Water Resources}
  • R. Mahmoudi*

    Numerous techniques are used in aquaculture to stripped eggs from fish broodstocks. The most common is the manual method, where the abdomen of the fish is gently massaged to extract the eggs. Alternatively, injecting gas pressure into the abdominal cavity can facilitate the release of eggs with potentially fewer negative impacts on the welfare and egg quality. This study compared the pneumatic stripping methods and the manual spawning method in rainbow trout (Oncorhynchus mykiss) spawners. The study focused on the effects of these techniques on the quality of the harvested eggs, relative fertility, stripping duration, and pH of the ovarian fluids. The findings revealed that maintaining a gas flow rate at 1.5 L min-1 during stripping is crucial to prevent post-stripping mortality of the broodstocks. The pneumatic method demonstrated a shorter duration of egg retrieval (39.5 s) compared to the manual method (42 s). Notably, there were no significant differences observed in ovarian fluid pH across the various methods (p<0.05). Overall, pneumatic egg extraction leads to higher-quality eggs with a greater hatching rate compared to the manual method. Considering the positive results of the pneumatic stripping and its high level of repeatability and efficiency, it can be used instead of the traditional method in rainbow trout hatcheries.

    Keywords: Hand Stripping, Pneumatic, Fish, Reproduction, Rainbow Trout}
  • S.D Hajimirrahimi*, J. Eghbali

    The main purpose of this research was to design a model that meets the educational needs of Iranian fisheries researchers in developing fisheries entrepreneurship ecosystems. In this research, educational needs were identified using a mixed research approach (qualitative-quantitative). In the first stage, semi-structured interviews and grounded theory were used to develop a model of fisheries researchers entrepreneurship education. The results indicated that casual conditions include coordination and communication challenges, applied research needed by the private sector, teaching general entrepreneurship skills, background conditions including the personal characteristics of researchers, the difficulties of carrying out organizational and research activities and financing challenges were intervening conditions. Supporting commercialization and establishing business acceleration centers, and presenting commercialization models as part of strategic conditions, promoting sustainable production and harvest, and recognizing the advantages of knowledge and income for researchers were identified as the positive effects of entrepreneurship education on Iranian fisheries researchers in the development of fisheries entrepreneurship ecosystems. In the quantitative section a questionnaire was compiled and given to a statistical sample which included researchers and faculty members of the Iranian Fisheries  Science Research Institute, Sampling was done through Cochran's formula (n=135). The identification of educational need items was accomplished through the employment of confirmatory factor analysis. Educational needs were categorized into seven components consist of 1) management and planning of applied research projects, 2) startup and entrepreneurship, 3) communication, 4) teamwork and networking, 5) fisheries Innovation ecosystem, 6) extension activities, principles and 7) rules and commercialization.

    Keywords: Fisheries Researchers, Ecosystem, Education, Entrepreneurship}
نکته
  • نتایج بر اساس تاریخ انتشار مرتب شده‌اند.
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  • در صورتی که می‌خواهید جستجو را در همه موضوعات و با شرایط دیگر تکرار کنید به صفحه جستجوی پیشرفته مجلات مراجعه کنید.
درخواست پشتیبانی - گزارش اشکال